Cargando…

Development of a One-Step Immunocapture Real-Time RT-PCR Assay for Detection of Tobacco Mosaic Virus in Soil

Tobacco mosaic virus (TMV) causes significant losses in many economically important crops. Contaminated soils may play roles as reservoirs and sources of transmission for TMV. In this study we report the development of an immunocapture real-time RT-PCR (IC-real-time RT-PCR) assay for direct detectio...

Descripción completa

Detalles Bibliográficos
Autores principales:	Yang, Jin-Guang, Wang, Feng-Long, Chen, De-Xin, Shen, Li-Li, Qian, Yu-Mei, Liang, Zhi-Yong, Zhou, Wen-Chang, Yan, Tai-He
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Molecular Diversity Preservation International (MDPI) 2012
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3571805/ https://www.ncbi.nlm.nih.gov/pubmed/23211755 http://dx.doi.org/10.3390/s121216685

Ejemplares similares

A one step real-time RT-PCR assay for the quantitation of Wheat yellow mosaic virus (WYMV)
por: Liu, Wenwen, et al.
Publicado: (2013)

A One-Step Real-Time RT-PCR Assay for the Detection and Quantitation of Sugarcane Streak Mosaic Virus
por: Fu, Wei-Lin, et al.
Publicado: (2015)

A Sensitive Immunocapture-RT-PCR Assay for Detection of Maize Iranian Mosaic Virus in Individual Small Brown Planthopper Vector, Laodelphax striatellus
por: Moeini, Pedram, et al.
Publicado: (2020)

One-step real-time RT-PCR assays for serotyping dengue virus in clinical samples
por: Alm, Erik, et al.
Publicado: (2015)

Development of a one step real time RT-PCR assay to detect and quantify Dugbe virus
por: Rodrigues, R., et al.
Publicado: (2011)

Development and Application of an Immunocapture PCR Diagnostic Assay Based on the Monoclonal Antibody for the Detection of Shigella
por: Zhang, Liding, et al.
Publicado: (2020)

Comprehensive Multiplex One-Step Real-Time TaqMan qRT-PCR Assays for Detection and Quantification of Hemorrhagic Fever Viruses
por: Pang, Zheng, et al.
Publicado: (2014)

Development of Immunocapture-LC/MS Assay for Simultaneous ADA Isotyping and Semiquantitation
por: Chen, Lin-Zhi, et al.
Publicado: (2016)

A one-step, one-tube real-time RT-PCR based assay with an automated analysis for detection of SARS-CoV-2
por: Dharavath, Bhasker, et al.
Publicado: (2020)

Development and Evaluation of a One-Step Quantitative RT-PCR Assay for Detection of Lassa Virus
por: Dedkov, Vladimir G., et al.
Publicado: (2019)

Comparison of a one-step real-time RT-PCR and a nested real-time RT-PCR for a genogroup II norovirus reveals differences in sensitivity depending upon assay design and visualization
por: Manuel, Clyde S., et al.
Publicado: (2021)

Development of a multiplex one step RT-PCR that detects eighteen respiratory viruses in clinical specimens and comparison with real time RT-PCR
por: Choudhary, Manohar L., et al.
Publicado: (2013)

Comparative Evaluation of Standard RT-PCR Assays and Commercial Real-Time RT-PCR Kits for Detection of Lassa Virus
por: Luo, Xue-Lian, et al.
Publicado: (2023)

Microparticle immunocapture assay for quantitation of protein multimer amount and size
por: Gutknecht, Michael F., et al.
Publicado: (2022)

A Broadly Reactive One-Step SYBR Green I Real-Time RT-PCR Assay for Rapid Detection of Murine Norovirus
por: Hanaki, Ken-Ichi, et al.
Publicado: (2014)

A novel multiplex one-step real-time RT-PCR assay for the simultaneous identification of enterovirus and parechovirus in clinical fecal samples
por: BUBBA, L., et al.
Publicado: (2015)

Genetic diversity of porcine reproductive and respiratory syndrome virus and evaluation of three one-step real-time RT-PCR assays in Korea
por: Shin, Go-Eun, et al.
Publicado: (2022)

Development of one-step SYBR Green real-time RT-PCR for quantifying bovine viral diarrhea virus type-1 and its comparison with conventional RT-PCR
por: Zhang, Ni, et al.
Publicado: (2011)

Development of quantitative multiplex RT-qPCR one step assay for detection of hepatitis delta virus
por: da Silva Queiroz, Jackson Alves, et al.
Publicado: (2023)

Development of a One-Step SYBR Green I Real-Time RT-PCR Assay for the Detection and Quantitation of Araraquara and Rio Mamore Hantavirus
por: Machado, Alex Martins, et al.
Publicado: (2013)

Comparison of the genexpert enterovirus assay (GXEA) with real-time one step RT-PCR for the detection of enteroviral RNA in the cerebrospinal fluid of patients with meningitis
por: Hong, JiYoung, et al.
Publicado: (2015)

Detection of Langat virus by TaqMan real-time one-step qRT-PCR method
por: Muhd Radzi, Siti Fatimah, et al.
Publicado: (2015)

Positive control synthesis method for COVID-19 diagnosis by one-step real-time RT-PCR
por: Cho, Hyejin, et al.
Publicado: (2020)

Detection of Cucumber green mottle mosaic virus in low-concentration virus-infected seeds by improved one-step pre-amplification RT-qPCR
por: Xinying, Yin, et al.
Publicado: (2022)

A Real-Time RT-PCR Assay for Genotyping of Rotavirus
por: Mousavi-Nasab, Seyed Dawood, et al.
Publicado: (2020)

A Single-Step Immunocapture Assay to Quantify HCC Exosomes Using the Highly Sensitive Fluorescence Nanoparticle-Tracking Analysis
por: Koksal, Ali Riza, et al.
Publicado: (2023)

Detection of a novel avian influenza A (H7N9) virus in humans by multiplex one-step real-time RT-PCR assay
por: Fan, Jian, et al.
Publicado: (2014)

Semiquantitative one-step RT-PCR for simultaneous identification of human influenza and respiratory syncytial viruses
por: Chi, X. Sherry, et al.
Publicado: (2007)

Quantification of Hantaan Virus with a SYBR Green Ⅰ-Based One-Step qRT-PCR Assay
por: Jiang, Wei, et al.
Publicado: (2013)

Detection of Shigella in Milk and Clinical Samples by Magnetic Immunocaptured-Loop-Mediated Isothermal Amplification Assay
por: Zhang, Liding, et al.
Publicado: (2018)

Immunocapture strategies in translational proteomics
por: Fredolini, Claudia, et al.
Publicado: (2016)

Application of a SYBR® Green One Step Real-time RT-PCR Assay to Detect Type 1 Porcine Reproductive and Respiratory Syndrome Virus
por: ISEKI, Hiroshi, et al.
Publicado: (2014)

Development and evaluation of a one-step real-time RT-PCR assay for universal detection of influenza A viruses from avian and mammal species
por: Nagy, Alexander, et al.
Publicado: (2010)

Viral load quantitation of SARS-coronavirus RNA using a one-step real-time RT-PCR
por: Keyaerts, Els, et al.
Publicado: (2006)

Correction: Quantification of Hantaan Virus with a SYBR Green Ⅰ-Based One-Step qRT-PCR Assay
por: Jiang, Wei, et al.
Publicado: (2013)

Evaluation of Two Triplex One-Step qRT-PCR Assays for the Quantification of Human Enteric Viruses in Environmental Samples
por: Farkas, Kata, et al.
Publicado: (2017)

One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus
por: LIU, Dafei, et al.
Publicado: (2018)

Development of a multiplex RT-PCR assay for simultaneous detection of Cucumber green mottle mosaic virus and Acidovorax citrulli in watermelon
por: Bi, Xinyue, et al.
Publicado: (2019)

The validation and utility of a quantitative one-step multiplex RT real-time PCR targeting Rotavirus A and Norovirus
por: Dung, Tran Thi Ngoc, et al.
Publicado: (2013)

Detection of HEV RNA Using One-Step Real-Time RT-PCR in Farrow-to-Finish Pig Farms in Bulgaria
por: Krumova-Valcheva, Gergana Lyubomirova, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_67arefogip66100c32a6djqljl