Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels

A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step pr...

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Detalles Bibliográficos
Autores principales: Ferro, Yannis, Perullini, Mercedes, Jobbagy, Matias, Bilmes, Sara A., Durrieu, Claude
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3571815/
https://www.ncbi.nlm.nih.gov/pubmed/23223083
http://dx.doi.org/10.3390/s121216879
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author Ferro, Yannis
Perullini, Mercedes
Jobbagy, Matias
Bilmes, Sara A.
Durrieu, Claude
author_facet Ferro, Yannis
Perullini, Mercedes
Jobbagy, Matias
Bilmes, Sara A.
Durrieu, Claude
author_sort Ferro, Yannis
collection PubMed
description A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step procedure. After studying the growth rate of entrapped cells, chlorophyll fluorescence was measured after exposure to (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU) and various concentrations of the common herbicide atrazine. Microalgae are very sensitive to herbicides and detection of fluorescence enhancement with very good efficiency was realized. The best detection limit was 0.1 μM, obtained with the strain C. reinhardtii after 40 minutes of exposure.
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spelling pubmed-35718152013-02-19 Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels Ferro, Yannis Perullini, Mercedes Jobbagy, Matias Bilmes, Sara A. Durrieu, Claude Sensors (Basel) Article A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step procedure. After studying the growth rate of entrapped cells, chlorophyll fluorescence was measured after exposure to (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU) and various concentrations of the common herbicide atrazine. Microalgae are very sensitive to herbicides and detection of fluorescence enhancement with very good efficiency was realized. The best detection limit was 0.1 μM, obtained with the strain C. reinhardtii after 40 minutes of exposure. Molecular Diversity Preservation International (MDPI) 2012-12-06 /pmc/articles/PMC3571815/ /pubmed/23223083 http://dx.doi.org/10.3390/s121216879 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Ferro, Yannis
Perullini, Mercedes
Jobbagy, Matias
Bilmes, Sara A.
Durrieu, Claude
Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels
title Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels
title_full Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels
title_fullStr Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels
title_full_unstemmed Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels
title_short Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels
title_sort development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3571815/
https://www.ncbi.nlm.nih.gov/pubmed/23223083
http://dx.doi.org/10.3390/s121216879
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