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MYADM controls endothelial barrier function through ERM-dependent regulation of ICAM-1 expression

The endothelium maintains a barrier between blood and tissue that becomes more permeable during inflammation. Membrane rafts are ordered assemblies of cholesterol, glycolipids, and proteins that modulate proinflammatory cell signaling and barrier function. In epithelial cells, the MAL family members...

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Autores principales: Aranda, Juan F., Reglero-Real, Natalia, Marcos-Ramiro, Beatriz, Ruiz-Sáenz, Ana, Fernández-Martín, Laura, Bernabé-Rubio, Miguel, Kremer, Leonor, Ridley, Anne J., Correas, Isabel, Alonso, Miguel A., Millán, Jaime
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3571871/
https://www.ncbi.nlm.nih.gov/pubmed/23264465
http://dx.doi.org/10.1091/mbc.E11-11-0914
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author Aranda, Juan F.
Reglero-Real, Natalia
Marcos-Ramiro, Beatriz
Ruiz-Sáenz, Ana
Fernández-Martín, Laura
Bernabé-Rubio, Miguel
Kremer, Leonor
Ridley, Anne J.
Correas, Isabel
Alonso, Miguel A.
Millán, Jaime
author_facet Aranda, Juan F.
Reglero-Real, Natalia
Marcos-Ramiro, Beatriz
Ruiz-Sáenz, Ana
Fernández-Martín, Laura
Bernabé-Rubio, Miguel
Kremer, Leonor
Ridley, Anne J.
Correas, Isabel
Alonso, Miguel A.
Millán, Jaime
author_sort Aranda, Juan F.
collection PubMed
description The endothelium maintains a barrier between blood and tissue that becomes more permeable during inflammation. Membrane rafts are ordered assemblies of cholesterol, glycolipids, and proteins that modulate proinflammatory cell signaling and barrier function. In epithelial cells, the MAL family members MAL, MAL2, and myeloid-associated differentiation marker (MYADM) regulate the function and dynamics of ordered membrane domains. We analyzed the expression of these three proteins in human endothelial cells and found that only MYADM is expressed. MYADM was confined in ordered domains at the plasma membrane, where it partially colocalized with filamentous actin and cell–cell junctions. Small interfering RNA (siRNA)-mediated MYADM knockdown increased permeability, ICAM-1 expression, and leukocyte adhesion, all of which are features of an inflammatory response. Barrier function decrease in MYADM-silenced cells was dependent on ICAM-1 expression. Membrane domains and the underlying actin cytoskeleton can regulate each other and are connected by ezrin, radixin, and moesin (ERM) proteins. In endothelial cells, MYADM knockdown induced ERM activation. Triple-ERM knockdown partially inhibited ICAM-1 increase induced by MYADM siRNA. Importantly, ERM knockdown also reduced ICAM-1 expression in response to the proinflammatory cytokine tumor necrosis factor-α. MYADM therefore regulates the connection between the plasma membrane and the cortical cytoskeleton and so can control the endothelial inflammatory response.
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spelling pubmed-35718712013-04-30 MYADM controls endothelial barrier function through ERM-dependent regulation of ICAM-1 expression Aranda, Juan F. Reglero-Real, Natalia Marcos-Ramiro, Beatriz Ruiz-Sáenz, Ana Fernández-Martín, Laura Bernabé-Rubio, Miguel Kremer, Leonor Ridley, Anne J. Correas, Isabel Alonso, Miguel A. Millán, Jaime Mol Biol Cell Articles The endothelium maintains a barrier between blood and tissue that becomes more permeable during inflammation. Membrane rafts are ordered assemblies of cholesterol, glycolipids, and proteins that modulate proinflammatory cell signaling and barrier function. In epithelial cells, the MAL family members MAL, MAL2, and myeloid-associated differentiation marker (MYADM) regulate the function and dynamics of ordered membrane domains. We analyzed the expression of these three proteins in human endothelial cells and found that only MYADM is expressed. MYADM was confined in ordered domains at the plasma membrane, where it partially colocalized with filamentous actin and cell–cell junctions. Small interfering RNA (siRNA)-mediated MYADM knockdown increased permeability, ICAM-1 expression, and leukocyte adhesion, all of which are features of an inflammatory response. Barrier function decrease in MYADM-silenced cells was dependent on ICAM-1 expression. Membrane domains and the underlying actin cytoskeleton can regulate each other and are connected by ezrin, radixin, and moesin (ERM) proteins. In endothelial cells, MYADM knockdown induced ERM activation. Triple-ERM knockdown partially inhibited ICAM-1 increase induced by MYADM siRNA. Importantly, ERM knockdown also reduced ICAM-1 expression in response to the proinflammatory cytokine tumor necrosis factor-α. MYADM therefore regulates the connection between the plasma membrane and the cortical cytoskeleton and so can control the endothelial inflammatory response. The American Society for Cell Biology 2013-02-15 /pmc/articles/PMC3571871/ /pubmed/23264465 http://dx.doi.org/10.1091/mbc.E11-11-0914 Text en © 2013 Aranda et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Aranda, Juan F.
Reglero-Real, Natalia
Marcos-Ramiro, Beatriz
Ruiz-Sáenz, Ana
Fernández-Martín, Laura
Bernabé-Rubio, Miguel
Kremer, Leonor
Ridley, Anne J.
Correas, Isabel
Alonso, Miguel A.
Millán, Jaime
MYADM controls endothelial barrier function through ERM-dependent regulation of ICAM-1 expression
title MYADM controls endothelial barrier function through ERM-dependent regulation of ICAM-1 expression
title_full MYADM controls endothelial barrier function through ERM-dependent regulation of ICAM-1 expression
title_fullStr MYADM controls endothelial barrier function through ERM-dependent regulation of ICAM-1 expression
title_full_unstemmed MYADM controls endothelial barrier function through ERM-dependent regulation of ICAM-1 expression
title_short MYADM controls endothelial barrier function through ERM-dependent regulation of ICAM-1 expression
title_sort myadm controls endothelial barrier function through erm-dependent regulation of icam-1 expression
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3571871/
https://www.ncbi.nlm.nih.gov/pubmed/23264465
http://dx.doi.org/10.1091/mbc.E11-11-0914
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