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Optimization of cultural conditions for conversion of glycerol to ethanol by Enterobacter aerogenes S012
The aim of this research is to optimize the cultural conditions for the conversion of glycerol to ethanol by Enterobacter aerogenes S012. Taguchi method was used to screen the cultural conditions based on their signal to noise ratio (SN). Temperature (°C), agitation speed (rpm) and time (h) were fou...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3571924/ https://www.ncbi.nlm.nih.gov/pubmed/23388539 http://dx.doi.org/10.1186/2191-0855-3-12 |
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author | Nwachukwu, Raymond E S Shahbazi, Abolghasem Wang, Lijun Worku, Mulumebet Ibrahim, Salam Schimmel, Keith |
author_facet | Nwachukwu, Raymond E S Shahbazi, Abolghasem Wang, Lijun Worku, Mulumebet Ibrahim, Salam Schimmel, Keith |
author_sort | Nwachukwu, Raymond E S |
collection | PubMed |
description | The aim of this research is to optimize the cultural conditions for the conversion of glycerol to ethanol by Enterobacter aerogenes S012. Taguchi method was used to screen the cultural conditions based on their signal to noise ratio (SN). Temperature (°C), agitation speed (rpm) and time (h) were found to have the highest influence on both glycerol utilization and ethanol production by the organism while pH had the lowest. Full factorial design, statistical analysis, and regression model equation were used to optimize the selected cultural parameters for maximum ethanol production. The result showed that fermentation at 38°C and 200 rpm for 48 h would be ideal for the bacteria to produce maximum amount of ethanol from glycerol. At these optimum conditions, ethanol production, yield and productivity were 25.4 g/l, 0.53 g/l/h, and 1.12 mol/mol-glycerol, repectively. Ethanol production increased to 26.5 g/l while yield and productivity decreased to 1.04 mol/mol-glycerol and 0.37 g/l/h, respectively, after 72 h. Analysis of the fermentation products was performed using HPLC, while anaerobic condition was created by purging the fermentation vessel with nitrogen gas. |
format | Online Article Text |
id | pubmed-3571924 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Springer |
record_format | MEDLINE/PubMed |
spelling | pubmed-35719242013-02-15 Optimization of cultural conditions for conversion of glycerol to ethanol by Enterobacter aerogenes S012 Nwachukwu, Raymond E S Shahbazi, Abolghasem Wang, Lijun Worku, Mulumebet Ibrahim, Salam Schimmel, Keith AMB Express Original Article The aim of this research is to optimize the cultural conditions for the conversion of glycerol to ethanol by Enterobacter aerogenes S012. Taguchi method was used to screen the cultural conditions based on their signal to noise ratio (SN). Temperature (°C), agitation speed (rpm) and time (h) were found to have the highest influence on both glycerol utilization and ethanol production by the organism while pH had the lowest. Full factorial design, statistical analysis, and regression model equation were used to optimize the selected cultural parameters for maximum ethanol production. The result showed that fermentation at 38°C and 200 rpm for 48 h would be ideal for the bacteria to produce maximum amount of ethanol from glycerol. At these optimum conditions, ethanol production, yield and productivity were 25.4 g/l, 0.53 g/l/h, and 1.12 mol/mol-glycerol, repectively. Ethanol production increased to 26.5 g/l while yield and productivity decreased to 1.04 mol/mol-glycerol and 0.37 g/l/h, respectively, after 72 h. Analysis of the fermentation products was performed using HPLC, while anaerobic condition was created by purging the fermentation vessel with nitrogen gas. Springer 2013-02-06 /pmc/articles/PMC3571924/ /pubmed/23388539 http://dx.doi.org/10.1186/2191-0855-3-12 Text en Copyright ©2013 Nwachukwu et al.; licensee Springer. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Nwachukwu, Raymond E S Shahbazi, Abolghasem Wang, Lijun Worku, Mulumebet Ibrahim, Salam Schimmel, Keith Optimization of cultural conditions for conversion of glycerol to ethanol by Enterobacter aerogenes S012 |
title | Optimization of cultural conditions for conversion of glycerol to ethanol by Enterobacter aerogenes S012 |
title_full | Optimization of cultural conditions for conversion of glycerol to ethanol by Enterobacter aerogenes S012 |
title_fullStr | Optimization of cultural conditions for conversion of glycerol to ethanol by Enterobacter aerogenes S012 |
title_full_unstemmed | Optimization of cultural conditions for conversion of glycerol to ethanol by Enterobacter aerogenes S012 |
title_short | Optimization of cultural conditions for conversion of glycerol to ethanol by Enterobacter aerogenes S012 |
title_sort | optimization of cultural conditions for conversion of glycerol to ethanol by enterobacter aerogenes s012 |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3571924/ https://www.ncbi.nlm.nih.gov/pubmed/23388539 http://dx.doi.org/10.1186/2191-0855-3-12 |
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