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Morphology and Nanomechanics of Sensory Neurons Growth Cones following Peripheral Nerve Injury
A prior peripheral nerve injury in vivo, promotes a rapid elongated mode of sensory neurons neurite regrowth in vitro. This in vitro model of conditioned axotomy allows analysis of the cellular and molecular mechanisms leading to an improved neurite re-growth. Our differential interference contrast...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3571950/ https://www.ncbi.nlm.nih.gov/pubmed/23418549 http://dx.doi.org/10.1371/journal.pone.0056286 |
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author | Martin, Marta Benzina, Ouafa Szabo, Vivien Végh, Attila-Gergely Lucas, Olivier Cloitre, Thierry Scamps, Frédérique Gergely, Csilla |
author_facet | Martin, Marta Benzina, Ouafa Szabo, Vivien Végh, Attila-Gergely Lucas, Olivier Cloitre, Thierry Scamps, Frédérique Gergely, Csilla |
author_sort | Martin, Marta |
collection | PubMed |
description | A prior peripheral nerve injury in vivo, promotes a rapid elongated mode of sensory neurons neurite regrowth in vitro. This in vitro model of conditioned axotomy allows analysis of the cellular and molecular mechanisms leading to an improved neurite re-growth. Our differential interference contrast microscopy and immunocytochemistry results show that conditioned axotomy, induced by sciatic nerve injury, did not increase somatic size of adult lumbar sensory neurons from mice dorsal root ganglia sensory neurons but promoted the appearance of larger neurites and growth cones. Using atomic force microscopy on live neurons, we investigated whether membrane mechanical properties of growth cones of axotomized neurons were modified following sciatic nerve injury. Our data revealed that neurons having a regenerative growth were characterized by softer growth cones, compared to control neurons. The increase of the growth cone membrane elasticity suggests a modification in the ratio and the inner framework of the main structural proteins. |
format | Online Article Text |
id | pubmed-3571950 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35719502013-02-15 Morphology and Nanomechanics of Sensory Neurons Growth Cones following Peripheral Nerve Injury Martin, Marta Benzina, Ouafa Szabo, Vivien Végh, Attila-Gergely Lucas, Olivier Cloitre, Thierry Scamps, Frédérique Gergely, Csilla PLoS One Research Article A prior peripheral nerve injury in vivo, promotes a rapid elongated mode of sensory neurons neurite regrowth in vitro. This in vitro model of conditioned axotomy allows analysis of the cellular and molecular mechanisms leading to an improved neurite re-growth. Our differential interference contrast microscopy and immunocytochemistry results show that conditioned axotomy, induced by sciatic nerve injury, did not increase somatic size of adult lumbar sensory neurons from mice dorsal root ganglia sensory neurons but promoted the appearance of larger neurites and growth cones. Using atomic force microscopy on live neurons, we investigated whether membrane mechanical properties of growth cones of axotomized neurons were modified following sciatic nerve injury. Our data revealed that neurons having a regenerative growth were characterized by softer growth cones, compared to control neurons. The increase of the growth cone membrane elasticity suggests a modification in the ratio and the inner framework of the main structural proteins. Public Library of Science 2013-02-13 /pmc/articles/PMC3571950/ /pubmed/23418549 http://dx.doi.org/10.1371/journal.pone.0056286 Text en © 2013 Martin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Martin, Marta Benzina, Ouafa Szabo, Vivien Végh, Attila-Gergely Lucas, Olivier Cloitre, Thierry Scamps, Frédérique Gergely, Csilla Morphology and Nanomechanics of Sensory Neurons Growth Cones following Peripheral Nerve Injury |
title | Morphology and Nanomechanics of Sensory Neurons Growth Cones following Peripheral Nerve Injury |
title_full | Morphology and Nanomechanics of Sensory Neurons Growth Cones following Peripheral Nerve Injury |
title_fullStr | Morphology and Nanomechanics of Sensory Neurons Growth Cones following Peripheral Nerve Injury |
title_full_unstemmed | Morphology and Nanomechanics of Sensory Neurons Growth Cones following Peripheral Nerve Injury |
title_short | Morphology and Nanomechanics of Sensory Neurons Growth Cones following Peripheral Nerve Injury |
title_sort | morphology and nanomechanics of sensory neurons growth cones following peripheral nerve injury |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3571950/ https://www.ncbi.nlm.nih.gov/pubmed/23418549 http://dx.doi.org/10.1371/journal.pone.0056286 |
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