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Oncogenic but non-essential role of N-myc downstream regulated gene 1 in the progression of esophageal squamous cell carcinoma

N-myc downstream regulated gene 1 (NDRG1/Cap43/Drg-1) has previously been shown to be dysregulated in esophageal squamous cell carcinoma (ESCC). In this study, we investigated the role of NDRG1 in the neoplastic progression of ESCC using ectopic gain-of-function and loss-of-function approaches. Stab...

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Autores principales: Wei, Wei, Bracher-Manecke, Jacqueline C., Zhao, Xiaohang, Davies, Neil H., Zhou, Lanping, Ai, Runna, Oliver, Lisa, Vallette, Francois, Hendricks, Denver T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3571998/
https://www.ncbi.nlm.nih.gov/pubmed/23192272
http://dx.doi.org/10.4161/cbt.22956
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author Wei, Wei
Bracher-Manecke, Jacqueline C.
Zhao, Xiaohang
Davies, Neil H.
Zhou, Lanping
Ai, Runna
Oliver, Lisa
Vallette, Francois
Hendricks, Denver T.
author_facet Wei, Wei
Bracher-Manecke, Jacqueline C.
Zhao, Xiaohang
Davies, Neil H.
Zhou, Lanping
Ai, Runna
Oliver, Lisa
Vallette, Francois
Hendricks, Denver T.
author_sort Wei, Wei
collection PubMed
description N-myc downstream regulated gene 1 (NDRG1/Cap43/Drg-1) has previously been shown to be dysregulated in esophageal squamous cell carcinoma (ESCC). In this study, we investigated the role of NDRG1 in the neoplastic progression of ESCC using ectopic gain-of-function and loss-of-function approaches. Stable transfectants of the KYSE30 ESCC cell line with altered NDRG1 levels were generated by lentiviral transduction. Although no measurable effects on in vitro cell proliferation were observed with altered NDRG1 expression, the ectopic overexpression of NDRG1 was positively linked to recognized markers of metastasis, angiogenesis and apoptotic evasion. Accordingly, in the nude mouse xenograft model system, NDRG1 overexpression promoted the in vivo growth of KYSE30 derived xenografts, which could be attributed to the reduced apoptotic and enhanced angiogenic activities associated with this gene. These processes were mediated in part by increased NFκB activity in NDRG1 overexpressing cells. Nevertheless, no significant phenotypic changes were observed in response to NDRG1 knock-down, suggesting that this gene might not be essential for the neoplastic progression of ESCC. Taken together, our results suggest that NDRG1 may play positive but dispensable roles in the progression of esophageal squamous cell carcinoma.
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spelling pubmed-35719982013-02-26 Oncogenic but non-essential role of N-myc downstream regulated gene 1 in the progression of esophageal squamous cell carcinoma Wei, Wei Bracher-Manecke, Jacqueline C. Zhao, Xiaohang Davies, Neil H. Zhou, Lanping Ai, Runna Oliver, Lisa Vallette, Francois Hendricks, Denver T. Cancer Biol Ther Research Paper N-myc downstream regulated gene 1 (NDRG1/Cap43/Drg-1) has previously been shown to be dysregulated in esophageal squamous cell carcinoma (ESCC). In this study, we investigated the role of NDRG1 in the neoplastic progression of ESCC using ectopic gain-of-function and loss-of-function approaches. Stable transfectants of the KYSE30 ESCC cell line with altered NDRG1 levels were generated by lentiviral transduction. Although no measurable effects on in vitro cell proliferation were observed with altered NDRG1 expression, the ectopic overexpression of NDRG1 was positively linked to recognized markers of metastasis, angiogenesis and apoptotic evasion. Accordingly, in the nude mouse xenograft model system, NDRG1 overexpression promoted the in vivo growth of KYSE30 derived xenografts, which could be attributed to the reduced apoptotic and enhanced angiogenic activities associated with this gene. These processes were mediated in part by increased NFκB activity in NDRG1 overexpressing cells. Nevertheless, no significant phenotypic changes were observed in response to NDRG1 knock-down, suggesting that this gene might not be essential for the neoplastic progression of ESCC. Taken together, our results suggest that NDRG1 may play positive but dispensable roles in the progression of esophageal squamous cell carcinoma. Landes Bioscience 2013-02-01 /pmc/articles/PMC3571998/ /pubmed/23192272 http://dx.doi.org/10.4161/cbt.22956 Text en Copyright © 2013 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Research Paper
Wei, Wei
Bracher-Manecke, Jacqueline C.
Zhao, Xiaohang
Davies, Neil H.
Zhou, Lanping
Ai, Runna
Oliver, Lisa
Vallette, Francois
Hendricks, Denver T.
Oncogenic but non-essential role of N-myc downstream regulated gene 1 in the progression of esophageal squamous cell carcinoma
title Oncogenic but non-essential role of N-myc downstream regulated gene 1 in the progression of esophageal squamous cell carcinoma
title_full Oncogenic but non-essential role of N-myc downstream regulated gene 1 in the progression of esophageal squamous cell carcinoma
title_fullStr Oncogenic but non-essential role of N-myc downstream regulated gene 1 in the progression of esophageal squamous cell carcinoma
title_full_unstemmed Oncogenic but non-essential role of N-myc downstream regulated gene 1 in the progression of esophageal squamous cell carcinoma
title_short Oncogenic but non-essential role of N-myc downstream regulated gene 1 in the progression of esophageal squamous cell carcinoma
title_sort oncogenic but non-essential role of n-myc downstream regulated gene 1 in the progression of esophageal squamous cell carcinoma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3571998/
https://www.ncbi.nlm.nih.gov/pubmed/23192272
http://dx.doi.org/10.4161/cbt.22956
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