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The h-Current in Periglomerular Dopaminergic Neurons of the Mouse Olfactory Bulb
The properties of the hyperpolarization-activated cation current (I(h)) were investigated in rat periglomerular dopaminergic neurons using patch-clamp recordings in thin slices. A reliable identification of single dopaminergic neurons was made possible by use of a transgenic line of mice expressing...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3572079/ https://www.ncbi.nlm.nih.gov/pubmed/23418585 http://dx.doi.org/10.1371/journal.pone.0056571 |
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author | Pignatelli, Angela Borin, Mirta Fogli Iseppe, Alex Gambardella, Cristina Belluzzi, Ottorino |
author_facet | Pignatelli, Angela Borin, Mirta Fogli Iseppe, Alex Gambardella, Cristina Belluzzi, Ottorino |
author_sort | Pignatelli, Angela |
collection | PubMed |
description | The properties of the hyperpolarization-activated cation current (I(h)) were investigated in rat periglomerular dopaminergic neurons using patch-clamp recordings in thin slices. A reliable identification of single dopaminergic neurons was made possible by use of a transgenic line of mice expressing eGFP under the tyrosine hydroxylase promoter. At 37 °C and minimizing the disturbance of the intracellular milieu with perforated patches, this current shows a midpoint of activation around −82.7 mV, with a significant level of opening already at rest, thereby giving a substantial contribution to the resting potential, and ultimately playing a relevant function in the control of the cell excitability. The blockage of I(h) has a profound influence on the spontaneous firing of these neurons, which result as strongly depressed. However the effect is not due to a direct role of the current in the pacemaker process, but to the I(h) influence on the resting membrane potential. I(h) kinetics is sensitive to the intracellular levels of cAMP, whose increase promotes a shift of the activation curve towards more positive potentials. The direct application of DA and 5-HT neurotransmitters, physiologically released onto bulbar dopaminergic neurons and known to act on metabotropic receptors coupled to the cAMP pathway, do not modifythe I(h) amplitude. On the contrary, noradrenaline almost halves the I(h) amplitude. Our data indicate that the HCN channels do not participate directly to the pacemaker activity of periglomerular dopaminergic neurons, but influence their resting membrane potential by controlling the excitability profile of these cells, and possibly affecting the processing of sensory information taking place at the entry of the bulbar circuitry. |
format | Online Article Text |
id | pubmed-3572079 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35720792013-02-15 The h-Current in Periglomerular Dopaminergic Neurons of the Mouse Olfactory Bulb Pignatelli, Angela Borin, Mirta Fogli Iseppe, Alex Gambardella, Cristina Belluzzi, Ottorino PLoS One Research Article The properties of the hyperpolarization-activated cation current (I(h)) were investigated in rat periglomerular dopaminergic neurons using patch-clamp recordings in thin slices. A reliable identification of single dopaminergic neurons was made possible by use of a transgenic line of mice expressing eGFP under the tyrosine hydroxylase promoter. At 37 °C and minimizing the disturbance of the intracellular milieu with perforated patches, this current shows a midpoint of activation around −82.7 mV, with a significant level of opening already at rest, thereby giving a substantial contribution to the resting potential, and ultimately playing a relevant function in the control of the cell excitability. The blockage of I(h) has a profound influence on the spontaneous firing of these neurons, which result as strongly depressed. However the effect is not due to a direct role of the current in the pacemaker process, but to the I(h) influence on the resting membrane potential. I(h) kinetics is sensitive to the intracellular levels of cAMP, whose increase promotes a shift of the activation curve towards more positive potentials. The direct application of DA and 5-HT neurotransmitters, physiologically released onto bulbar dopaminergic neurons and known to act on metabotropic receptors coupled to the cAMP pathway, do not modifythe I(h) amplitude. On the contrary, noradrenaline almost halves the I(h) amplitude. Our data indicate that the HCN channels do not participate directly to the pacemaker activity of periglomerular dopaminergic neurons, but influence their resting membrane potential by controlling the excitability profile of these cells, and possibly affecting the processing of sensory information taking place at the entry of the bulbar circuitry. Public Library of Science 2013-02-13 /pmc/articles/PMC3572079/ /pubmed/23418585 http://dx.doi.org/10.1371/journal.pone.0056571 Text en © 2013 Pignatelli et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Pignatelli, Angela Borin, Mirta Fogli Iseppe, Alex Gambardella, Cristina Belluzzi, Ottorino The h-Current in Periglomerular Dopaminergic Neurons of the Mouse Olfactory Bulb |
title | The h-Current in Periglomerular Dopaminergic Neurons of the Mouse Olfactory Bulb |
title_full | The h-Current in Periglomerular Dopaminergic Neurons of the Mouse Olfactory Bulb |
title_fullStr | The h-Current in Periglomerular Dopaminergic Neurons of the Mouse Olfactory Bulb |
title_full_unstemmed | The h-Current in Periglomerular Dopaminergic Neurons of the Mouse Olfactory Bulb |
title_short | The h-Current in Periglomerular Dopaminergic Neurons of the Mouse Olfactory Bulb |
title_sort | h-current in periglomerular dopaminergic neurons of the mouse olfactory bulb |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3572079/ https://www.ncbi.nlm.nih.gov/pubmed/23418585 http://dx.doi.org/10.1371/journal.pone.0056571 |
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