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Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated Pancytopenia
Previously, we described a group of patients with hemocytopenia who did not conform to diagnostic criteria of known hematological and nonhematological diseases. Most patients responded well to adrenocortical hormone and/or high-dose intravenous immunoglobulin treatment, indicating that cytopenia mig...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3572650/ https://www.ncbi.nlm.nih.gov/pubmed/23424599 http://dx.doi.org/10.1155/2013/297678 |
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author | Liu, Hui Fu, Rong Wang, Yihao Liu, Hong Li, Lijuan Wang, Honglei Chen, Jin Yu, Hong Shao, Zonghong |
author_facet | Liu, Hui Fu, Rong Wang, Yihao Liu, Hong Li, Lijuan Wang, Honglei Chen, Jin Yu, Hong Shao, Zonghong |
author_sort | Liu, Hui |
collection | PubMed |
description | Previously, we described a group of patients with hemocytopenia who did not conform to diagnostic criteria of known hematological and nonhematological diseases. Most patients responded well to adrenocortical hormone and/or high-dose intravenous immunoglobulin treatment, indicating that cytopenia might be mediated by autoantibodies. Autoantibodies were detected on the membrane of various bone marrow (BM) hemopoietic cells by bone marrow mononuclear-cell-Coombs test or flow cytometric analysis. Thus, the hemocytopenia was termed “Immunorelated Pancytopenia” (IRP) to distinguish it from other pancytopenias. Autoantigens in IRP were investigated by membrane protein extraction from BM hemopoietic cells and BM supernatant from IRP patients. Autoantibody IgG was detected in the BM supernatant of 75% of patients (15/20), which was significantly higher than that in aplastic anemia, myelodysplastic syndrome, or autoimmune hemolytic anemia patients (0%) and normal healthy controls (0%) (P < 0.01). Autoantigens had approximate molecular weights of 25, 30, 47.5, 60, 65, 70, and 80 kDa, some of which were further identified by mass fingerprinting. This study identified that a G-protein-coupled receptor 156 variant and chain P, a crystal structure of the cytoplasmic domain of human erythrocyte band-3 protein, were autoantigens in IRP. Further studies are needed to confirm the antigenicity of these autoantigens. |
format | Online Article Text |
id | pubmed-3572650 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-35726502013-02-19 Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated Pancytopenia Liu, Hui Fu, Rong Wang, Yihao Liu, Hong Li, Lijuan Wang, Honglei Chen, Jin Yu, Hong Shao, Zonghong Clin Dev Immunol Research Article Previously, we described a group of patients with hemocytopenia who did not conform to diagnostic criteria of known hematological and nonhematological diseases. Most patients responded well to adrenocortical hormone and/or high-dose intravenous immunoglobulin treatment, indicating that cytopenia might be mediated by autoantibodies. Autoantibodies were detected on the membrane of various bone marrow (BM) hemopoietic cells by bone marrow mononuclear-cell-Coombs test or flow cytometric analysis. Thus, the hemocytopenia was termed “Immunorelated Pancytopenia” (IRP) to distinguish it from other pancytopenias. Autoantigens in IRP were investigated by membrane protein extraction from BM hemopoietic cells and BM supernatant from IRP patients. Autoantibody IgG was detected in the BM supernatant of 75% of patients (15/20), which was significantly higher than that in aplastic anemia, myelodysplastic syndrome, or autoimmune hemolytic anemia patients (0%) and normal healthy controls (0%) (P < 0.01). Autoantigens had approximate molecular weights of 25, 30, 47.5, 60, 65, 70, and 80 kDa, some of which were further identified by mass fingerprinting. This study identified that a G-protein-coupled receptor 156 variant and chain P, a crystal structure of the cytoplasmic domain of human erythrocyte band-3 protein, were autoantigens in IRP. Further studies are needed to confirm the antigenicity of these autoantigens. Hindawi Publishing Corporation 2013 2013-01-31 /pmc/articles/PMC3572650/ /pubmed/23424599 http://dx.doi.org/10.1155/2013/297678 Text en Copyright © 2013 Hui Liu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Liu, Hui Fu, Rong Wang, Yihao Liu, Hong Li, Lijuan Wang, Honglei Chen, Jin Yu, Hong Shao, Zonghong Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated Pancytopenia |
title | Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated Pancytopenia |
title_full | Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated Pancytopenia |
title_fullStr | Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated Pancytopenia |
title_full_unstemmed | Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated Pancytopenia |
title_short | Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated Pancytopenia |
title_sort | detection and analysis of autoantigens targeted by autoantibodies in immunorelated pancytopenia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3572650/ https://www.ncbi.nlm.nih.gov/pubmed/23424599 http://dx.doi.org/10.1155/2013/297678 |
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