Constructing a Mouse Oct4 Promoter/EGFP Vector, as a Whole-Cellular Reporter to Monitor the Pluripotent State of Cells

BACKGROUND: The transcription factor Oct-4, is an important marker of undifferentiating level and a key regulating factor for maintenance of pluripotency in cells. Establishment of an Oct-4 promoter-based reporter system is an appropriate tool for monitoring the differentiation of embryonic stem cel...

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Detalles Bibliográficos
Autores principales: Ghorbani, Reza, Emamzadeh, Abdolrahman, Khazaie, Yahya, Dormiani, Kianoush, Ghaedi, Kamran, Rabbani, Mohammad, Foruzanfar, Mahboubeh, Karbalaie, Khadijeh, Karamali, Fereshteh, Lachinani, Liana, Kiani-Esfahani, Abbas, Nematollahi, Marzieh, Esfahani, Mohammad Hossein Nasr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Avicenna Research Institute 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3572702/
https://www.ncbi.nlm.nih.gov/pubmed/23626871
Descripción
Sumario:BACKGROUND: The transcription factor Oct-4, is an important marker of undifferentiating level and a key regulating factor for maintenance of pluripotency in cells. Establishment of an Oct-4 promoter-based reporter system is an appropriate tool for monitoring the differentiation of embryonic stem cells both in vivo and in vitro. METHODS: In the present study, we report construction of a recombinant vector, pDB2 Oct4 promoter/EGFP, in which expression of Enhanced Green Fluorescent Protein (EGFP) was controlled by the mouse Oct-4 promoter. RESULTS: In transfected mouse embryonic stem cells with this vector, EGFP was predicted to be specifically expressed in pluripotency state. After transfection, high-level expression of EGFP under the control of Oct-4 promoter was observed in manipulated embryonic stem cells. CONCLUSION: Thus, our new cellular reporter showed that both the properties of embryonic cells and expression the EGFP could be of great help in studying the differentiating and reprogramming mechanisms of mESCs.