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Involvement of Rab28 in NF-κB Nuclear Transport in Endothelial Cells

Our previous proteomic analysis revealed the expression of Rab28 in arteries of rats. However, the function of Rab28 in mammalian cells, and its role in vessels are still unknown. Coarctation of abdominal aorta above left kidney artery in rat was used as hypertensive animal model. FX-4000 cyclic str...

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Autores principales: Jiang, Jun, Qi, Ying-Xin, Zhang, Ping, Gu, Wen-Tian, Yan, Zhi-Qiang, Shen, Bao-Rong, Yao, Qing-Ping, Kong, Han, Chien, Shu, Jiang, Zong-Lai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3573041/
https://www.ncbi.nlm.nih.gov/pubmed/23457503
http://dx.doi.org/10.1371/journal.pone.0056076
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author Jiang, Jun
Qi, Ying-Xin
Zhang, Ping
Gu, Wen-Tian
Yan, Zhi-Qiang
Shen, Bao-Rong
Yao, Qing-Ping
Kong, Han
Chien, Shu
Jiang, Zong-Lai
author_facet Jiang, Jun
Qi, Ying-Xin
Zhang, Ping
Gu, Wen-Tian
Yan, Zhi-Qiang
Shen, Bao-Rong
Yao, Qing-Ping
Kong, Han
Chien, Shu
Jiang, Zong-Lai
author_sort Jiang, Jun
collection PubMed
description Our previous proteomic analysis revealed the expression of Rab28 in arteries of rats. However, the function of Rab28 in mammalian cells, and its role in vessels are still unknown. Coarctation of abdominal aorta above left kidney artery in rat was used as hypertensive animal model. FX-4000 cyclic strain loading system was used to mimic the mechanical condition on vascular cells during hypertension in vitro. Immunofluorescence and co-immunoprecipitation (Co-IP) were used to identify distribution and interaction of Rab28 and nuclear factor kappa B (NF-κB). Rab28 expression was significantly increased in carotid arteries of hypertensive rats. High cyclic strain induced Rab28 expression of endothelial cells (ECs) through a paracrine control of vascular smooth muscles cells (VSMCs), which at least partly via angiotensin II (Ang II). Rab28 knockdown decreased proliferation of ECs, while increased apoptosis and migration. Immunofluorescence revealed that Ang II stimulated the co-translocation of Rab28 and NF-κB from cytoplasm into nucleus. Knockdown of Rab28 attenuated NF-κB activation. Co-IP of NF-κB p65 and Rab28 indicated their interaction. Our results revealed that Rab28, as a novel regulator of NF-κB nuclear transport, might participate in the disturbance of EC homeostasis.
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spelling pubmed-35730412013-03-01 Involvement of Rab28 in NF-κB Nuclear Transport in Endothelial Cells Jiang, Jun Qi, Ying-Xin Zhang, Ping Gu, Wen-Tian Yan, Zhi-Qiang Shen, Bao-Rong Yao, Qing-Ping Kong, Han Chien, Shu Jiang, Zong-Lai PLoS One Research Article Our previous proteomic analysis revealed the expression of Rab28 in arteries of rats. However, the function of Rab28 in mammalian cells, and its role in vessels are still unknown. Coarctation of abdominal aorta above left kidney artery in rat was used as hypertensive animal model. FX-4000 cyclic strain loading system was used to mimic the mechanical condition on vascular cells during hypertension in vitro. Immunofluorescence and co-immunoprecipitation (Co-IP) were used to identify distribution and interaction of Rab28 and nuclear factor kappa B (NF-κB). Rab28 expression was significantly increased in carotid arteries of hypertensive rats. High cyclic strain induced Rab28 expression of endothelial cells (ECs) through a paracrine control of vascular smooth muscles cells (VSMCs), which at least partly via angiotensin II (Ang II). Rab28 knockdown decreased proliferation of ECs, while increased apoptosis and migration. Immunofluorescence revealed that Ang II stimulated the co-translocation of Rab28 and NF-κB from cytoplasm into nucleus. Knockdown of Rab28 attenuated NF-κB activation. Co-IP of NF-κB p65 and Rab28 indicated their interaction. Our results revealed that Rab28, as a novel regulator of NF-κB nuclear transport, might participate in the disturbance of EC homeostasis. Public Library of Science 2013-02-14 /pmc/articles/PMC3573041/ /pubmed/23457503 http://dx.doi.org/10.1371/journal.pone.0056076 Text en © 2013 Jiang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Jiang, Jun
Qi, Ying-Xin
Zhang, Ping
Gu, Wen-Tian
Yan, Zhi-Qiang
Shen, Bao-Rong
Yao, Qing-Ping
Kong, Han
Chien, Shu
Jiang, Zong-Lai
Involvement of Rab28 in NF-κB Nuclear Transport in Endothelial Cells
title Involvement of Rab28 in NF-κB Nuclear Transport in Endothelial Cells
title_full Involvement of Rab28 in NF-κB Nuclear Transport in Endothelial Cells
title_fullStr Involvement of Rab28 in NF-κB Nuclear Transport in Endothelial Cells
title_full_unstemmed Involvement of Rab28 in NF-κB Nuclear Transport in Endothelial Cells
title_short Involvement of Rab28 in NF-κB Nuclear Transport in Endothelial Cells
title_sort involvement of rab28 in nf-κb nuclear transport in endothelial cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3573041/
https://www.ncbi.nlm.nih.gov/pubmed/23457503
http://dx.doi.org/10.1371/journal.pone.0056076
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