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Neutrophils Exert a Suppressive Effect on Th1 Responses to Intracellular Pathogen Brucella abortus
Polymorphonuclear neutrophils (PMNs) are the first line of defense against microbial pathogens. In addition to their role in innate immunity, PMNs may also regulate events related to adaptive immunity. To investigate the influence of PMNs in the immune response during chronic bacterial infections, w...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3573106/ https://www.ncbi.nlm.nih.gov/pubmed/23458832 http://dx.doi.org/10.1371/journal.ppat.1003167 |
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author | Barquero-Calvo, Elías Martirosyan, Anna Ordoñez-Rueda, Diana Arce-Gorvel, Vilma Alfaro-Alarcón, Alejandro Lepidi, Hubert Malissen, Bernard Malissen, Marie Gorvel, Jean-Pierre Moreno, Edgardo |
author_facet | Barquero-Calvo, Elías Martirosyan, Anna Ordoñez-Rueda, Diana Arce-Gorvel, Vilma Alfaro-Alarcón, Alejandro Lepidi, Hubert Malissen, Bernard Malissen, Marie Gorvel, Jean-Pierre Moreno, Edgardo |
author_sort | Barquero-Calvo, Elías |
collection | PubMed |
description | Polymorphonuclear neutrophils (PMNs) are the first line of defense against microbial pathogens. In addition to their role in innate immunity, PMNs may also regulate events related to adaptive immunity. To investigate the influence of PMNs in the immune response during chronic bacterial infections, we explored the course of brucellosis in antibody PMN-depleted C57BL/6 mice and in neutropenic mutant Genista mouse model. We demonstrate that at later times of infection, Brucella abortus is killed more efficiently in the absence of PMNs than in their presence. The higher bacterial removal was concomitant to the: i) comparatively reduced spleen swelling; ii) augmented infiltration of epithelioid histiocytes corresponding to macrophages/dendritic cells (DCs); iii) higher recruitment of monocytes and monocyte/DCs phenotype; iv) significant activation of B and T lymphocytes, and v) increased levels of INF-γ and negligible levels of IL4 indicating a balance of Th1 over Th2 response. These results reveal that PMNs have an unexpected influence in dampening the immune response against intracellular Brucella infection and strengthen the notion that PMNs actively participate in regulatory circuits shaping both innate and adaptive immunity. |
format | Online Article Text |
id | pubmed-3573106 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35731062013-03-01 Neutrophils Exert a Suppressive Effect on Th1 Responses to Intracellular Pathogen Brucella abortus Barquero-Calvo, Elías Martirosyan, Anna Ordoñez-Rueda, Diana Arce-Gorvel, Vilma Alfaro-Alarcón, Alejandro Lepidi, Hubert Malissen, Bernard Malissen, Marie Gorvel, Jean-Pierre Moreno, Edgardo PLoS Pathog Research Article Polymorphonuclear neutrophils (PMNs) are the first line of defense against microbial pathogens. In addition to their role in innate immunity, PMNs may also regulate events related to adaptive immunity. To investigate the influence of PMNs in the immune response during chronic bacterial infections, we explored the course of brucellosis in antibody PMN-depleted C57BL/6 mice and in neutropenic mutant Genista mouse model. We demonstrate that at later times of infection, Brucella abortus is killed more efficiently in the absence of PMNs than in their presence. The higher bacterial removal was concomitant to the: i) comparatively reduced spleen swelling; ii) augmented infiltration of epithelioid histiocytes corresponding to macrophages/dendritic cells (DCs); iii) higher recruitment of monocytes and monocyte/DCs phenotype; iv) significant activation of B and T lymphocytes, and v) increased levels of INF-γ and negligible levels of IL4 indicating a balance of Th1 over Th2 response. These results reveal that PMNs have an unexpected influence in dampening the immune response against intracellular Brucella infection and strengthen the notion that PMNs actively participate in regulatory circuits shaping both innate and adaptive immunity. Public Library of Science 2013-02-14 /pmc/articles/PMC3573106/ /pubmed/23458832 http://dx.doi.org/10.1371/journal.ppat.1003167 Text en © 2013 Barquero-Calvo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Barquero-Calvo, Elías Martirosyan, Anna Ordoñez-Rueda, Diana Arce-Gorvel, Vilma Alfaro-Alarcón, Alejandro Lepidi, Hubert Malissen, Bernard Malissen, Marie Gorvel, Jean-Pierre Moreno, Edgardo Neutrophils Exert a Suppressive Effect on Th1 Responses to Intracellular Pathogen Brucella abortus |
title | Neutrophils Exert a Suppressive Effect on Th1 Responses to Intracellular Pathogen Brucella abortus
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title_full | Neutrophils Exert a Suppressive Effect on Th1 Responses to Intracellular Pathogen Brucella abortus
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title_fullStr | Neutrophils Exert a Suppressive Effect on Th1 Responses to Intracellular Pathogen Brucella abortus
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title_full_unstemmed | Neutrophils Exert a Suppressive Effect on Th1 Responses to Intracellular Pathogen Brucella abortus
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title_short | Neutrophils Exert a Suppressive Effect on Th1 Responses to Intracellular Pathogen Brucella abortus
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title_sort | neutrophils exert a suppressive effect on th1 responses to intracellular pathogen brucella abortus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3573106/ https://www.ncbi.nlm.nih.gov/pubmed/23458832 http://dx.doi.org/10.1371/journal.ppat.1003167 |
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