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Agr genes, missing in amniotes, are involved in the body appendages regeneration in frog tadpoles
Previous studies have shown that Agr genes, which encode thioredoxin domain-containing secreted proteins, play a critical role in limb regeneration in salamanders. To determine the evolutionary conservation of Agr function, it is important to examine whether Agrs play a similar role in species with...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3573343/ https://www.ncbi.nlm.nih.gov/pubmed/23412115 http://dx.doi.org/10.1038/srep01279 |
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author | Ivanova, Anastasiya S. Tereshina, Maria B. Ermakova, Galina V. Belousov, Vsevolod V. Zaraisky, Andrey G. |
author_facet | Ivanova, Anastasiya S. Tereshina, Maria B. Ermakova, Galina V. Belousov, Vsevolod V. Zaraisky, Andrey G. |
author_sort | Ivanova, Anastasiya S. |
collection | PubMed |
description | Previous studies have shown that Agr genes, which encode thioredoxin domain-containing secreted proteins, play a critical role in limb regeneration in salamanders. To determine the evolutionary conservation of Agr function, it is important to examine whether Agrs play a similar role in species with a different type of regeneration. Here, we refined the phylogeny of Agrs, revealing three subfamilies: Ag1, Agr2 and Agr3. Importantly, we established that Ag1 was lost in higher vertebrates, which correlates with their decreased regeneration ability. In Xenopus laevis tadpoles (anamniotes), which have all three Agr subfamilies and a high regenerating capacity, Agrs were activated in the stumps of tails and hindlimb buds that were amputated at stage 52. However, Agrs were not up-regulated when the hindlimb buds were amputated at stage 57, the stage at which their regeneration capacity is lost. Our findings indicate the general importance of Agrs for body appendages regeneration in amphibians. |
format | Online Article Text |
id | pubmed-3573343 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-35733432013-02-15 Agr genes, missing in amniotes, are involved in the body appendages regeneration in frog tadpoles Ivanova, Anastasiya S. Tereshina, Maria B. Ermakova, Galina V. Belousov, Vsevolod V. Zaraisky, Andrey G. Sci Rep Article Previous studies have shown that Agr genes, which encode thioredoxin domain-containing secreted proteins, play a critical role in limb regeneration in salamanders. To determine the evolutionary conservation of Agr function, it is important to examine whether Agrs play a similar role in species with a different type of regeneration. Here, we refined the phylogeny of Agrs, revealing three subfamilies: Ag1, Agr2 and Agr3. Importantly, we established that Ag1 was lost in higher vertebrates, which correlates with their decreased regeneration ability. In Xenopus laevis tadpoles (anamniotes), which have all three Agr subfamilies and a high regenerating capacity, Agrs were activated in the stumps of tails and hindlimb buds that were amputated at stage 52. However, Agrs were not up-regulated when the hindlimb buds were amputated at stage 57, the stage at which their regeneration capacity is lost. Our findings indicate the general importance of Agrs for body appendages regeneration in amphibians. Nature Publishing Group 2013-02-15 /pmc/articles/PMC3573343/ /pubmed/23412115 http://dx.doi.org/10.1038/srep01279 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Ivanova, Anastasiya S. Tereshina, Maria B. Ermakova, Galina V. Belousov, Vsevolod V. Zaraisky, Andrey G. Agr genes, missing in amniotes, are involved in the body appendages regeneration in frog tadpoles |
title | Agr genes, missing in amniotes, are involved in the body appendages regeneration in frog tadpoles |
title_full | Agr genes, missing in amniotes, are involved in the body appendages regeneration in frog tadpoles |
title_fullStr | Agr genes, missing in amniotes, are involved in the body appendages regeneration in frog tadpoles |
title_full_unstemmed | Agr genes, missing in amniotes, are involved in the body appendages regeneration in frog tadpoles |
title_short | Agr genes, missing in amniotes, are involved in the body appendages regeneration in frog tadpoles |
title_sort | agr genes, missing in amniotes, are involved in the body appendages regeneration in frog tadpoles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3573343/ https://www.ncbi.nlm.nih.gov/pubmed/23412115 http://dx.doi.org/10.1038/srep01279 |
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