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Characterization of nasopharyngeal isolates of type b Haemophilus influenzae from Delhi
BACKGROUND & OBJECTIVES: Haemophilus influenzae is an important cause of mortality and morbidity among young children in developing countries. Increasing incidence of antibiotic resistance especially production of extended spectrum beta lactamase (ESBL) has made treatment and management of H. in...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3573609/ https://www.ncbi.nlm.nih.gov/pubmed/23287135 |
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author | Saikia, Kandarpa K. Das, Bimal K. Bewal, Ramesh K. Kapil, Arti Arora, N.K. Sood, Seema |
author_facet | Saikia, Kandarpa K. Das, Bimal K. Bewal, Ramesh K. Kapil, Arti Arora, N.K. Sood, Seema |
author_sort | Saikia, Kandarpa K. |
collection | PubMed |
description | BACKGROUND & OBJECTIVES: Haemophilus influenzae is an important cause of mortality and morbidity among young children in developing countries. Increasing incidence of antibiotic resistance especially production of extended spectrum beta lactamase (ESBL) has made treatment and management of H. influenzae infection more difficult. Nasopharyngeal H. influenzae isolates are excellent surrogate for determination of antibiotic resistance prevalent among invasive H. influenzae isolates. In this study, we characterized nasopharyngeal H. influenzae isolates obtained from healthy school going children in Delhi. METHODS: Nasopharyngeal H. influenzae isolates were collected from healthy school going children and subjected to serotyping, fimbrial typing and antibiogram profiling. ESBL production was recorded using phenotypic as well as molecular methods. Multi locus sequence typing (MLST) of 13 representative nasopharyngeal H. influenzae isolates was performed as per guidelines. RESULTS: A significant proportion (26 of 80, 32.5%) of nasopharyngeal isolates of H. influenzae were identified as serotype b. Fimbrial gene (hifA) was detected in 23 (28.75%) isolates. Resistance against commonly prescribed antibiotics (Amp, Tet, Chloro, Septran, Cephalexin) were observed to be high among the nasopharyngeal commensal H. influenzae. Extended spectrum beta lactamase (ESBL) production was observed in a five (6.25%) isolates by both double disk diffusion and molecular typing. MLST identified several novel alleles as well as novel sequence types. INTERPRETATION & CONCLUSIONS: Our findings showed high resistance against common antibiotics and detection of ESBL in nasopharyngeal H. influenzae isolates collected from normal healthy school going children in Delhi. Detection of H. influenzae type b capsular gene and the presence of fimbrial gene (hif A) suggest virulence potential of these isolates. Discovery of novel alleles and presence of new sequence types (STs) among nasopharyngeal H. influenzae isolates may suggest wider genetic diversity. |
format | Online Article Text |
id | pubmed-3573609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-35736092013-02-22 Characterization of nasopharyngeal isolates of type b Haemophilus influenzae from Delhi Saikia, Kandarpa K. Das, Bimal K. Bewal, Ramesh K. Kapil, Arti Arora, N.K. Sood, Seema Indian J Med Res Original Article BACKGROUND & OBJECTIVES: Haemophilus influenzae is an important cause of mortality and morbidity among young children in developing countries. Increasing incidence of antibiotic resistance especially production of extended spectrum beta lactamase (ESBL) has made treatment and management of H. influenzae infection more difficult. Nasopharyngeal H. influenzae isolates are excellent surrogate for determination of antibiotic resistance prevalent among invasive H. influenzae isolates. In this study, we characterized nasopharyngeal H. influenzae isolates obtained from healthy school going children in Delhi. METHODS: Nasopharyngeal H. influenzae isolates were collected from healthy school going children and subjected to serotyping, fimbrial typing and antibiogram profiling. ESBL production was recorded using phenotypic as well as molecular methods. Multi locus sequence typing (MLST) of 13 representative nasopharyngeal H. influenzae isolates was performed as per guidelines. RESULTS: A significant proportion (26 of 80, 32.5%) of nasopharyngeal isolates of H. influenzae were identified as serotype b. Fimbrial gene (hifA) was detected in 23 (28.75%) isolates. Resistance against commonly prescribed antibiotics (Amp, Tet, Chloro, Septran, Cephalexin) were observed to be high among the nasopharyngeal commensal H. influenzae. Extended spectrum beta lactamase (ESBL) production was observed in a five (6.25%) isolates by both double disk diffusion and molecular typing. MLST identified several novel alleles as well as novel sequence types. INTERPRETATION & CONCLUSIONS: Our findings showed high resistance against common antibiotics and detection of ESBL in nasopharyngeal H. influenzae isolates collected from normal healthy school going children in Delhi. Detection of H. influenzae type b capsular gene and the presence of fimbrial gene (hif A) suggest virulence potential of these isolates. Discovery of novel alleles and presence of new sequence types (STs) among nasopharyngeal H. influenzae isolates may suggest wider genetic diversity. Medknow Publications & Media Pvt Ltd 2012-11 /pmc/articles/PMC3573609/ /pubmed/23287135 Text en Copyright: © The Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Saikia, Kandarpa K. Das, Bimal K. Bewal, Ramesh K. Kapil, Arti Arora, N.K. Sood, Seema Characterization of nasopharyngeal isolates of type b Haemophilus influenzae from Delhi |
title | Characterization of nasopharyngeal isolates of type b Haemophilus influenzae from Delhi |
title_full | Characterization of nasopharyngeal isolates of type b Haemophilus influenzae from Delhi |
title_fullStr | Characterization of nasopharyngeal isolates of type b Haemophilus influenzae from Delhi |
title_full_unstemmed | Characterization of nasopharyngeal isolates of type b Haemophilus influenzae from Delhi |
title_short | Characterization of nasopharyngeal isolates of type b Haemophilus influenzae from Delhi |
title_sort | characterization of nasopharyngeal isolates of type b haemophilus influenzae from delhi |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3573609/ https://www.ncbi.nlm.nih.gov/pubmed/23287135 |
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