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Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L.) seeds at two developmental stages
BACKGROUND: Sacha Inchi (Plukenetia volubilis L., Euphorbiaceae) is a potential oilseed crop because the seeds of this plant are rich in unsaturated fatty acids (FAs). In particular, the fatty acid composition of its seed oil differs markedly in containing large quantities of α-linolenic acid (18C:3...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3574040/ https://www.ncbi.nlm.nih.gov/pubmed/23256450 http://dx.doi.org/10.1186/1471-2164-13-716 |
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author | Wang, Xiaojuan Xu, Ronghua Wang, Ruling Liu, Aizhong |
author_facet | Wang, Xiaojuan Xu, Ronghua Wang, Ruling Liu, Aizhong |
author_sort | Wang, Xiaojuan |
collection | PubMed |
description | BACKGROUND: Sacha Inchi (Plukenetia volubilis L., Euphorbiaceae) is a potential oilseed crop because the seeds of this plant are rich in unsaturated fatty acids (FAs). In particular, the fatty acid composition of its seed oil differs markedly in containing large quantities of α-linolenic acid (18C:3, a kind of ω-3 FAs). However, little is known about the molecular mechanisms responsible for biosynthesis of unsaturated fatty acids in the developing seeds of this species. Transcriptome data are needed to better understand these mechanisms. RESULTS: In this study, de novo transcriptome assembly and gene expression analysis were performed using Illumina sequencing technology. A total of 52.6 million 90-bp paired-end reads were generated from two libraries constructed at the initial stage and fast oil accumulation stage of seed development. These reads were assembled into 70,392 unigenes; 22,179 unigenes showed a 2-fold or greater expression difference between the two libraries. Using this data we identified unigenes that may be involved in de novo FA and triacylglycerol biosynthesis. In particular, a number of unigenes encoding desaturase for formation of unsaturated fatty acids with high expression levels in the fast oil accumulation stage compared with the initial stage of seed development were identified. CONCLUSIONS: This study provides the first comprehensive dataset characterizing Sacha Inchi gene expression at the transcriptional level. These data provide the foundation for further studies on molecular mechanisms underlying oil accumulation and PUFA biosynthesis in Sacha Inchi seeds. Our analyses facilitate understanding of the molecular mechanisms responsible for the high unsaturated fatty acids (especially α-linolenic acid) accumulation in Sacha Inchi seeds. |
format | Online Article Text |
id | pubmed-3574040 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35740402013-02-20 Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L.) seeds at two developmental stages Wang, Xiaojuan Xu, Ronghua Wang, Ruling Liu, Aizhong BMC Genomics Research Article BACKGROUND: Sacha Inchi (Plukenetia volubilis L., Euphorbiaceae) is a potential oilseed crop because the seeds of this plant are rich in unsaturated fatty acids (FAs). In particular, the fatty acid composition of its seed oil differs markedly in containing large quantities of α-linolenic acid (18C:3, a kind of ω-3 FAs). However, little is known about the molecular mechanisms responsible for biosynthesis of unsaturated fatty acids in the developing seeds of this species. Transcriptome data are needed to better understand these mechanisms. RESULTS: In this study, de novo transcriptome assembly and gene expression analysis were performed using Illumina sequencing technology. A total of 52.6 million 90-bp paired-end reads were generated from two libraries constructed at the initial stage and fast oil accumulation stage of seed development. These reads were assembled into 70,392 unigenes; 22,179 unigenes showed a 2-fold or greater expression difference between the two libraries. Using this data we identified unigenes that may be involved in de novo FA and triacylglycerol biosynthesis. In particular, a number of unigenes encoding desaturase for formation of unsaturated fatty acids with high expression levels in the fast oil accumulation stage compared with the initial stage of seed development were identified. CONCLUSIONS: This study provides the first comprehensive dataset characterizing Sacha Inchi gene expression at the transcriptional level. These data provide the foundation for further studies on molecular mechanisms underlying oil accumulation and PUFA biosynthesis in Sacha Inchi seeds. Our analyses facilitate understanding of the molecular mechanisms responsible for the high unsaturated fatty acids (especially α-linolenic acid) accumulation in Sacha Inchi seeds. BioMed Central 2012-12-20 /pmc/articles/PMC3574040/ /pubmed/23256450 http://dx.doi.org/10.1186/1471-2164-13-716 Text en Copyright ©2012 Wang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Xiaojuan Xu, Ronghua Wang, Ruling Liu, Aizhong Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L.) seeds at two developmental stages |
title | Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L.) seeds at two developmental stages |
title_full | Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L.) seeds at two developmental stages |
title_fullStr | Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L.) seeds at two developmental stages |
title_full_unstemmed | Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L.) seeds at two developmental stages |
title_short | Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L.) seeds at two developmental stages |
title_sort | transcriptome analysis of sacha inchi (plukenetia volubilis l.) seeds at two developmental stages |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3574040/ https://www.ncbi.nlm.nih.gov/pubmed/23256450 http://dx.doi.org/10.1186/1471-2164-13-716 |
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