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Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L.) seeds at two developmental stages

BACKGROUND: Sacha Inchi (Plukenetia volubilis L., Euphorbiaceae) is a potential oilseed crop because the seeds of this plant are rich in unsaturated fatty acids (FAs). In particular, the fatty acid composition of its seed oil differs markedly in containing large quantities of α-linolenic acid (18C:3...

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Autores principales: Wang, Xiaojuan, Xu, Ronghua, Wang, Ruling, Liu, Aizhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3574040/
https://www.ncbi.nlm.nih.gov/pubmed/23256450
http://dx.doi.org/10.1186/1471-2164-13-716
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author Wang, Xiaojuan
Xu, Ronghua
Wang, Ruling
Liu, Aizhong
author_facet Wang, Xiaojuan
Xu, Ronghua
Wang, Ruling
Liu, Aizhong
author_sort Wang, Xiaojuan
collection PubMed
description BACKGROUND: Sacha Inchi (Plukenetia volubilis L., Euphorbiaceae) is a potential oilseed crop because the seeds of this plant are rich in unsaturated fatty acids (FAs). In particular, the fatty acid composition of its seed oil differs markedly in containing large quantities of α-linolenic acid (18C:3, a kind of ω-3 FAs). However, little is known about the molecular mechanisms responsible for biosynthesis of unsaturated fatty acids in the developing seeds of this species. Transcriptome data are needed to better understand these mechanisms. RESULTS: In this study, de novo transcriptome assembly and gene expression analysis were performed using Illumina sequencing technology. A total of 52.6 million 90-bp paired-end reads were generated from two libraries constructed at the initial stage and fast oil accumulation stage of seed development. These reads were assembled into 70,392 unigenes; 22,179 unigenes showed a 2-fold or greater expression difference between the two libraries. Using this data we identified unigenes that may be involved in de novo FA and triacylglycerol biosynthesis. In particular, a number of unigenes encoding desaturase for formation of unsaturated fatty acids with high expression levels in the fast oil accumulation stage compared with the initial stage of seed development were identified. CONCLUSIONS: This study provides the first comprehensive dataset characterizing Sacha Inchi gene expression at the transcriptional level. These data provide the foundation for further studies on molecular mechanisms underlying oil accumulation and PUFA biosynthesis in Sacha Inchi seeds. Our analyses facilitate understanding of the molecular mechanisms responsible for the high unsaturated fatty acids (especially α-linolenic acid) accumulation in Sacha Inchi seeds.
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spelling pubmed-35740402013-02-20 Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L.) seeds at two developmental stages Wang, Xiaojuan Xu, Ronghua Wang, Ruling Liu, Aizhong BMC Genomics Research Article BACKGROUND: Sacha Inchi (Plukenetia volubilis L., Euphorbiaceae) is a potential oilseed crop because the seeds of this plant are rich in unsaturated fatty acids (FAs). In particular, the fatty acid composition of its seed oil differs markedly in containing large quantities of α-linolenic acid (18C:3, a kind of ω-3 FAs). However, little is known about the molecular mechanisms responsible for biosynthesis of unsaturated fatty acids in the developing seeds of this species. Transcriptome data are needed to better understand these mechanisms. RESULTS: In this study, de novo transcriptome assembly and gene expression analysis were performed using Illumina sequencing technology. A total of 52.6 million 90-bp paired-end reads were generated from two libraries constructed at the initial stage and fast oil accumulation stage of seed development. These reads were assembled into 70,392 unigenes; 22,179 unigenes showed a 2-fold or greater expression difference between the two libraries. Using this data we identified unigenes that may be involved in de novo FA and triacylglycerol biosynthesis. In particular, a number of unigenes encoding desaturase for formation of unsaturated fatty acids with high expression levels in the fast oil accumulation stage compared with the initial stage of seed development were identified. CONCLUSIONS: This study provides the first comprehensive dataset characterizing Sacha Inchi gene expression at the transcriptional level. These data provide the foundation for further studies on molecular mechanisms underlying oil accumulation and PUFA biosynthesis in Sacha Inchi seeds. Our analyses facilitate understanding of the molecular mechanisms responsible for the high unsaturated fatty acids (especially α-linolenic acid) accumulation in Sacha Inchi seeds. BioMed Central 2012-12-20 /pmc/articles/PMC3574040/ /pubmed/23256450 http://dx.doi.org/10.1186/1471-2164-13-716 Text en Copyright ©2012 Wang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Xiaojuan
Xu, Ronghua
Wang, Ruling
Liu, Aizhong
Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L.) seeds at two developmental stages
title Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L.) seeds at two developmental stages
title_full Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L.) seeds at two developmental stages
title_fullStr Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L.) seeds at two developmental stages
title_full_unstemmed Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L.) seeds at two developmental stages
title_short Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L.) seeds at two developmental stages
title_sort transcriptome analysis of sacha inchi (plukenetia volubilis l.) seeds at two developmental stages
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3574040/
https://www.ncbi.nlm.nih.gov/pubmed/23256450
http://dx.doi.org/10.1186/1471-2164-13-716
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