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OpenCFU, a New Free and Open-Source Software to Count Cell Colonies and Other Circular Objects

Counting circular objects such as cell colonies is an important source of information for biologists. Although this task is often time-consuming and subjective, it is still predominantly performed manually. The aim of the present work is to provide a new tool to enumerate circular objects from digit...

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Detalles Bibliográficos
Autor principal: Geissmann, Quentin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3574151/
https://www.ncbi.nlm.nih.gov/pubmed/23457446
http://dx.doi.org/10.1371/journal.pone.0054072
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author Geissmann, Quentin
author_facet Geissmann, Quentin
author_sort Geissmann, Quentin
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description Counting circular objects such as cell colonies is an important source of information for biologists. Although this task is often time-consuming and subjective, it is still predominantly performed manually. The aim of the present work is to provide a new tool to enumerate circular objects from digital pictures and video streams. Here, I demonstrate that the created program, OpenCFU, is very robust, accurate and fast. In addition, it provides control over the processing parameters and is implemented in an intuitive and modern interface. OpenCFU is a cross-platform and open-source software freely available at http://opencfu.sourceforge.net.
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spelling pubmed-35741512013-03-01 OpenCFU, a New Free and Open-Source Software to Count Cell Colonies and Other Circular Objects Geissmann, Quentin PLoS One Research Article Counting circular objects such as cell colonies is an important source of information for biologists. Although this task is often time-consuming and subjective, it is still predominantly performed manually. The aim of the present work is to provide a new tool to enumerate circular objects from digital pictures and video streams. Here, I demonstrate that the created program, OpenCFU, is very robust, accurate and fast. In addition, it provides control over the processing parameters and is implemented in an intuitive and modern interface. OpenCFU is a cross-platform and open-source software freely available at http://opencfu.sourceforge.net. Public Library of Science 2013-02-15 /pmc/articles/PMC3574151/ /pubmed/23457446 http://dx.doi.org/10.1371/journal.pone.0054072 Text en © 2013 Quentin Geissmann http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Geissmann, Quentin
OpenCFU, a New Free and Open-Source Software to Count Cell Colonies and Other Circular Objects
title OpenCFU, a New Free and Open-Source Software to Count Cell Colonies and Other Circular Objects
title_full OpenCFU, a New Free and Open-Source Software to Count Cell Colonies and Other Circular Objects
title_fullStr OpenCFU, a New Free and Open-Source Software to Count Cell Colonies and Other Circular Objects
title_full_unstemmed OpenCFU, a New Free and Open-Source Software to Count Cell Colonies and Other Circular Objects
title_short OpenCFU, a New Free and Open-Source Software to Count Cell Colonies and Other Circular Objects
title_sort opencfu, a new free and open-source software to count cell colonies and other circular objects
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3574151/
https://www.ncbi.nlm.nih.gov/pubmed/23457446
http://dx.doi.org/10.1371/journal.pone.0054072
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