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Flow Cytometry in the Detection of Neonatal Sepsis

Neonatal sepsis remains a burden problem by showing minimal initial symptoms of subtle character, nonspecific manifestation, and diagnostic pitfalls. The clinical course can be fulminant and fatal if treatment is not commenced promptly. It is therefore crucial to establish early diagnosis and initia...

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Autores principales: Umlauf, Volker N., Dreschers, Stephan, Orlikowsky, Thorsten W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3574650/
https://www.ncbi.nlm.nih.gov/pubmed/23431318
http://dx.doi.org/10.1155/2013/763191
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author Umlauf, Volker N.
Dreschers, Stephan
Orlikowsky, Thorsten W.
author_facet Umlauf, Volker N.
Dreschers, Stephan
Orlikowsky, Thorsten W.
author_sort Umlauf, Volker N.
collection PubMed
description Neonatal sepsis remains a burden problem by showing minimal initial symptoms of subtle character, nonspecific manifestation, and diagnostic pitfalls. The clinical course can be fulminant and fatal if treatment is not commenced promptly. It is therefore crucial to establish early diagnosis and initiate adequate therapy. Besides clinical symptoms, the most reliable laboratory markers in establishing diagnosis is currently the combined measurement of CRP and a cytokine (IL-6 and IL-8). Due to their different kinetics, a diagnostic gap might occur and thus withholding antimicrobial therapy in clinical suspicion of infection is not acceptable. We therefore need parameters which unerringly differentiate between infants in need for antimicrobial therapy and those who are not. Flow cytometry promises to be a useful tool in this field, allowing the determination of different cellular, dissolved, and functional pathophysiological components of sepsis. Despite technical and methodical advances in flow cytometry, its use in clinical routine is still limited. Advantages and disadvantages of promising new parameters in diagnosis of sepsis performed by flow cytometry, particularly CD64, HLA-DR, and apoptosis, are reviewed here. The necessity of tests to be used as an “ideal” parameter is presented.
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spelling pubmed-35746502013-02-21 Flow Cytometry in the Detection of Neonatal Sepsis Umlauf, Volker N. Dreschers, Stephan Orlikowsky, Thorsten W. Int J Pediatr Review Article Neonatal sepsis remains a burden problem by showing minimal initial symptoms of subtle character, nonspecific manifestation, and diagnostic pitfalls. The clinical course can be fulminant and fatal if treatment is not commenced promptly. It is therefore crucial to establish early diagnosis and initiate adequate therapy. Besides clinical symptoms, the most reliable laboratory markers in establishing diagnosis is currently the combined measurement of CRP and a cytokine (IL-6 and IL-8). Due to their different kinetics, a diagnostic gap might occur and thus withholding antimicrobial therapy in clinical suspicion of infection is not acceptable. We therefore need parameters which unerringly differentiate between infants in need for antimicrobial therapy and those who are not. Flow cytometry promises to be a useful tool in this field, allowing the determination of different cellular, dissolved, and functional pathophysiological components of sepsis. Despite technical and methodical advances in flow cytometry, its use in clinical routine is still limited. Advantages and disadvantages of promising new parameters in diagnosis of sepsis performed by flow cytometry, particularly CD64, HLA-DR, and apoptosis, are reviewed here. The necessity of tests to be used as an “ideal” parameter is presented. Hindawi Publishing Corporation 2013 2013-02-03 /pmc/articles/PMC3574650/ /pubmed/23431318 http://dx.doi.org/10.1155/2013/763191 Text en Copyright © 2013 Volker N. Umlauf et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Umlauf, Volker N.
Dreschers, Stephan
Orlikowsky, Thorsten W.
Flow Cytometry in the Detection of Neonatal Sepsis
title Flow Cytometry in the Detection of Neonatal Sepsis
title_full Flow Cytometry in the Detection of Neonatal Sepsis
title_fullStr Flow Cytometry in the Detection of Neonatal Sepsis
title_full_unstemmed Flow Cytometry in the Detection of Neonatal Sepsis
title_short Flow Cytometry in the Detection of Neonatal Sepsis
title_sort flow cytometry in the detection of neonatal sepsis
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3574650/
https://www.ncbi.nlm.nih.gov/pubmed/23431318
http://dx.doi.org/10.1155/2013/763191
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