Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets

tRNA-derived RNA fragments (tRFs) are 19mer small RNAs that associate with Argonaute (AGO) proteins in humans. However, in plants, it is unknown if tRFs bind with AGO proteins. Here, using public deep sequencing libraries of immunoprecipitated Argonaute proteins (AGO-IP) and bioinformatics approache...

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Autores principales: Loss-Morais, Guilherme, Waterhouse, Peter M, Margis, Rogerio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Discovery Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3574835/
https://www.ncbi.nlm.nih.gov/pubmed/23402430
http://dx.doi.org/10.1186/1745-6150-8-6
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author Loss-Morais, Guilherme
Waterhouse, Peter M
Margis, Rogerio
author_facet Loss-Morais, Guilherme
Waterhouse, Peter M
Margis, Rogerio
author_sort Loss-Morais, Guilherme
collection PubMed
description tRNA-derived RNA fragments (tRFs) are 19mer small RNAs that associate with Argonaute (AGO) proteins in humans. However, in plants, it is unknown if tRFs bind with AGO proteins. Here, using public deep sequencing libraries of immunoprecipitated Argonaute proteins (AGO-IP) and bioinformatics approaches, we identified the Arabidopsis thaliana AGO-IP tRFs. Moreover, using three degradome deep sequencing libraries, we identified four putative tRF targets. The expression pattern of tRFs, based on deep sequencing data, was also analyzed under abiotic and biotic stresses. The results obtained here represent a useful starting point for future studies on tRFs in plants.
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spelling pubmed-35748352013-02-18 Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets Loss-Morais, Guilherme Waterhouse, Peter M Margis, Rogerio Biol Direct Discovery Notes tRNA-derived RNA fragments (tRFs) are 19mer small RNAs that associate with Argonaute (AGO) proteins in humans. However, in plants, it is unknown if tRFs bind with AGO proteins. Here, using public deep sequencing libraries of immunoprecipitated Argonaute proteins (AGO-IP) and bioinformatics approaches, we identified the Arabidopsis thaliana AGO-IP tRFs. Moreover, using three degradome deep sequencing libraries, we identified four putative tRF targets. The expression pattern of tRFs, based on deep sequencing data, was also analyzed under abiotic and biotic stresses. The results obtained here represent a useful starting point for future studies on tRFs in plants. BioMed Central 2013-02-12 /pmc/articles/PMC3574835/ /pubmed/23402430 http://dx.doi.org/10.1186/1745-6150-8-6 Text en Copyright ©2013 Loss-Morais et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Discovery Notes
Loss-Morais, Guilherme
Waterhouse, Peter M
Margis, Rogerio
Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
title Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
title_full Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
title_fullStr Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
title_full_unstemmed Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
title_short Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
title_sort description of plant trna-derived rna fragments (trfs) associated with argonaute and identification of their putative targets
topic Discovery Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3574835/
https://www.ncbi.nlm.nih.gov/pubmed/23402430
http://dx.doi.org/10.1186/1745-6150-8-6
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