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Characterization of murine macrophages from bone marrow, spleen and peritoneum

BACKGROUND: Macrophages have heterogeneous phenotypes and complex functions within both innate and adaptive immune responses. To date, most experimental studies have been performed on macrophages derived from bone marrow, spleen and peritoneum. However, differences among macrophages from these parti...

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Autores principales: Wang, Changqi, Yu, Xiao, Cao, Qi, Wang, Ya, Zheng, Guoping, Tan, Thian Kui, Zhao, Hong, Zhao, Ye, Wang, Yiping, Harris, David CH
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3574850/
https://www.ncbi.nlm.nih.gov/pubmed/23384230
http://dx.doi.org/10.1186/1471-2172-14-6
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author Wang, Changqi
Yu, Xiao
Cao, Qi
Wang, Ya
Zheng, Guoping
Tan, Thian Kui
Zhao, Hong
Zhao, Ye
Wang, Yiping
Harris, David CH
author_facet Wang, Changqi
Yu, Xiao
Cao, Qi
Wang, Ya
Zheng, Guoping
Tan, Thian Kui
Zhao, Hong
Zhao, Ye
Wang, Yiping
Harris, David CH
author_sort Wang, Changqi
collection PubMed
description BACKGROUND: Macrophages have heterogeneous phenotypes and complex functions within both innate and adaptive immune responses. To date, most experimental studies have been performed on macrophages derived from bone marrow, spleen and peritoneum. However, differences among macrophages from these particular sources remain unclear. In this study, the features of murine macrophages from bone marrow, spleen and peritoneum were compared. RESULTS: We found that peritoneal macrophages (PMs) appear to be more mature than bone marrow derived macrophages (BMs) and splenic macrophages (SPMs) based on their morphology and surface molecular characteristics. BMs showed the strongest capacity for both proliferation and phagocytosis among the three populations of macrophage. Under resting conditions, SPMs maintained high levels of pro-inflammatory cytokines expression (IL-6, IL-12 and TNF-α), whereas BMs produced high levels of suppressive cytokines (IL-10 and TGF-β). However, SPMs activated with LPS not only maintained higher levels of (IL-6, IL-12 and TNF-α) than BMs or PMs, but also maintained higher levels of IL-10 and TGF-β. CONCLUSIONS: Our results show that BMs, SPMs and PMs are distinct populations with different biological functions, providing clues to guide their further experimental or therapeutic use.
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spelling pubmed-35748502013-02-18 Characterization of murine macrophages from bone marrow, spleen and peritoneum Wang, Changqi Yu, Xiao Cao, Qi Wang, Ya Zheng, Guoping Tan, Thian Kui Zhao, Hong Zhao, Ye Wang, Yiping Harris, David CH BMC Immunol Research Article BACKGROUND: Macrophages have heterogeneous phenotypes and complex functions within both innate and adaptive immune responses. To date, most experimental studies have been performed on macrophages derived from bone marrow, spleen and peritoneum. However, differences among macrophages from these particular sources remain unclear. In this study, the features of murine macrophages from bone marrow, spleen and peritoneum were compared. RESULTS: We found that peritoneal macrophages (PMs) appear to be more mature than bone marrow derived macrophages (BMs) and splenic macrophages (SPMs) based on their morphology and surface molecular characteristics. BMs showed the strongest capacity for both proliferation and phagocytosis among the three populations of macrophage. Under resting conditions, SPMs maintained high levels of pro-inflammatory cytokines expression (IL-6, IL-12 and TNF-α), whereas BMs produced high levels of suppressive cytokines (IL-10 and TGF-β). However, SPMs activated with LPS not only maintained higher levels of (IL-6, IL-12 and TNF-α) than BMs or PMs, but also maintained higher levels of IL-10 and TGF-β. CONCLUSIONS: Our results show that BMs, SPMs and PMs are distinct populations with different biological functions, providing clues to guide their further experimental or therapeutic use. BioMed Central 2013-02-05 /pmc/articles/PMC3574850/ /pubmed/23384230 http://dx.doi.org/10.1186/1471-2172-14-6 Text en Copyright ©2013 Wang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Changqi
Yu, Xiao
Cao, Qi
Wang, Ya
Zheng, Guoping
Tan, Thian Kui
Zhao, Hong
Zhao, Ye
Wang, Yiping
Harris, David CH
Characterization of murine macrophages from bone marrow, spleen and peritoneum
title Characterization of murine macrophages from bone marrow, spleen and peritoneum
title_full Characterization of murine macrophages from bone marrow, spleen and peritoneum
title_fullStr Characterization of murine macrophages from bone marrow, spleen and peritoneum
title_full_unstemmed Characterization of murine macrophages from bone marrow, spleen and peritoneum
title_short Characterization of murine macrophages from bone marrow, spleen and peritoneum
title_sort characterization of murine macrophages from bone marrow, spleen and peritoneum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3574850/
https://www.ncbi.nlm.nih.gov/pubmed/23384230
http://dx.doi.org/10.1186/1471-2172-14-6
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