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Development of a sensitive RT-PCR method for amplifying and sequencing near full-length HCV genotype 1 RNA from patient samples

BACKGROUND: Direct-acting antiviral (DAAs) agents for hepatitis C virus (HCV) span a variety of targets, including proteins encoded by the NS3/4A, NS4B, NS5A, and NS5B genes. Treatment with DAAs has been shown to select variants with sequence changes in the HCV genome encoding amino acids that may c...

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Detalles Bibliográficos
Autores principales:	Zhang, Eileen Z, Bartels, Doug J, Frantz, J Dan, Seepersaud, Sheila, Lippke, Judith A, Shames, Benjamin, Zhou, Yi, Lin, Chao, Kwong, Ann, Kieffer, Tara L
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2013
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575352/ https://www.ncbi.nlm.nih.gov/pubmed/23402332 http://dx.doi.org/10.1186/1743-422X-10-53

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