Regulation of Spine Density and Morphology by IQGAP1 Protein Domains

IQGAP1 is a scaffolding protein that regulates spine number. We now show a differential role for IQGAP1 domains in spine morphogenesis, in which a region of the N-terminus that promotes Arp2/3-mediated actin polymerization and branching stimulates spine head formation while a region that binds to Cd...

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Detalles Bibliográficos
Autores principales: Jausoro, Ignacio, Mestres, Ivan, Quassollo, Gonzalo, Masseroni, Lujan, Heredia, Florencia, Caceres, Alfredo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575492/
https://www.ncbi.nlm.nih.gov/pubmed/23441206
http://dx.doi.org/10.1371/journal.pone.0056574
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author Jausoro, Ignacio
Mestres, Ivan
Quassollo, Gonzalo
Masseroni, Lujan
Heredia, Florencia
Caceres, Alfredo
author_facet Jausoro, Ignacio
Mestres, Ivan
Quassollo, Gonzalo
Masseroni, Lujan
Heredia, Florencia
Caceres, Alfredo
author_sort Jausoro, Ignacio
collection PubMed
description IQGAP1 is a scaffolding protein that regulates spine number. We now show a differential role for IQGAP1 domains in spine morphogenesis, in which a region of the N-terminus that promotes Arp2/3-mediated actin polymerization and branching stimulates spine head formation while a region that binds to Cdc42 and Rac is required for stalk extension. Conversely, IQGAP1 rescues spine deficiency induced by expression of dominant negative Cdc42 by stimulating formation of stubby spines. Together, our observations place IQGAP1 as a crucial regulator of spine number and shape acting through the N-Wasp Arp2/3 complex, as well as upstream and downstream of Cdc42.
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spelling pubmed-35754922013-02-25 Regulation of Spine Density and Morphology by IQGAP1 Protein Domains Jausoro, Ignacio Mestres, Ivan Quassollo, Gonzalo Masseroni, Lujan Heredia, Florencia Caceres, Alfredo PLoS One Research Article IQGAP1 is a scaffolding protein that regulates spine number. We now show a differential role for IQGAP1 domains in spine morphogenesis, in which a region of the N-terminus that promotes Arp2/3-mediated actin polymerization and branching stimulates spine head formation while a region that binds to Cdc42 and Rac is required for stalk extension. Conversely, IQGAP1 rescues spine deficiency induced by expression of dominant negative Cdc42 by stimulating formation of stubby spines. Together, our observations place IQGAP1 as a crucial regulator of spine number and shape acting through the N-Wasp Arp2/3 complex, as well as upstream and downstream of Cdc42. Public Library of Science 2013-02-18 /pmc/articles/PMC3575492/ /pubmed/23441206 http://dx.doi.org/10.1371/journal.pone.0056574 Text en © 2013 Jausoro et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Jausoro, Ignacio
Mestres, Ivan
Quassollo, Gonzalo
Masseroni, Lujan
Heredia, Florencia
Caceres, Alfredo
Regulation of Spine Density and Morphology by IQGAP1 Protein Domains
title Regulation of Spine Density and Morphology by IQGAP1 Protein Domains
title_full Regulation of Spine Density and Morphology by IQGAP1 Protein Domains
title_fullStr Regulation of Spine Density and Morphology by IQGAP1 Protein Domains
title_full_unstemmed Regulation of Spine Density and Morphology by IQGAP1 Protein Domains
title_short Regulation of Spine Density and Morphology by IQGAP1 Protein Domains
title_sort regulation of spine density and morphology by iqgap1 protein domains
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575492/
https://www.ncbi.nlm.nih.gov/pubmed/23441206
http://dx.doi.org/10.1371/journal.pone.0056574
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