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Condensin II initiates sister chromatid resolution during S phase

Condensins I and II are multisubunit complexes that play essential yet distinct functions in chromosome condensation and segregation in mitosis. Unlike condensin I, condensin II localizes to the nucleus during interphase, but it remains poorly understood what functions condensin II might have before...

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Detalles Bibliográficos
Autores principales: Ono, Takao, Yamashita, Daisuke, Hirano, Tatsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575537/
https://www.ncbi.nlm.nih.gov/pubmed/23401001
http://dx.doi.org/10.1083/jcb.201208008
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author Ono, Takao
Yamashita, Daisuke
Hirano, Tatsuya
author_facet Ono, Takao
Yamashita, Daisuke
Hirano, Tatsuya
author_sort Ono, Takao
collection PubMed
description Condensins I and II are multisubunit complexes that play essential yet distinct functions in chromosome condensation and segregation in mitosis. Unlike condensin I, condensin II localizes to the nucleus during interphase, but it remains poorly understood what functions condensin II might have before mitotic entry. Here, we report that condensin II changes its chromatin-binding property during S phase. Remarkably, advanced premature chromosome condensation (PCC) assays enabled us to visualize condensin II forming “sister axes” in replicated regions of chromosomes in S phase cells. Depletion of condensin II compromised PCC-driven sister chromatid resolution during S phase. Moreover, fluorescence in situ hybridization assays revealed that condensin II, but not condensin I, promotes disjoining duplicated chromosomal loci during S phase. Application of mild replicative stress partially impaired this process and further exacerbated phenotypes arising from condensin II depletion. Our results suggest that condensin II initiates structural reorganization of duplicated chromosomes during S phase to prepare for their proper condensation and segregation in mitosis.
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spelling pubmed-35755372013-08-18 Condensin II initiates sister chromatid resolution during S phase Ono, Takao Yamashita, Daisuke Hirano, Tatsuya J Cell Biol Research Articles Condensins I and II are multisubunit complexes that play essential yet distinct functions in chromosome condensation and segregation in mitosis. Unlike condensin I, condensin II localizes to the nucleus during interphase, but it remains poorly understood what functions condensin II might have before mitotic entry. Here, we report that condensin II changes its chromatin-binding property during S phase. Remarkably, advanced premature chromosome condensation (PCC) assays enabled us to visualize condensin II forming “sister axes” in replicated regions of chromosomes in S phase cells. Depletion of condensin II compromised PCC-driven sister chromatid resolution during S phase. Moreover, fluorescence in situ hybridization assays revealed that condensin II, but not condensin I, promotes disjoining duplicated chromosomal loci during S phase. Application of mild replicative stress partially impaired this process and further exacerbated phenotypes arising from condensin II depletion. Our results suggest that condensin II initiates structural reorganization of duplicated chromosomes during S phase to prepare for their proper condensation and segregation in mitosis. The Rockefeller University Press 2013-02-18 /pmc/articles/PMC3575537/ /pubmed/23401001 http://dx.doi.org/10.1083/jcb.201208008 Text en © 2013 Ono et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Ono, Takao
Yamashita, Daisuke
Hirano, Tatsuya
Condensin II initiates sister chromatid resolution during S phase
title Condensin II initiates sister chromatid resolution during S phase
title_full Condensin II initiates sister chromatid resolution during S phase
title_fullStr Condensin II initiates sister chromatid resolution during S phase
title_full_unstemmed Condensin II initiates sister chromatid resolution during S phase
title_short Condensin II initiates sister chromatid resolution during S phase
title_sort condensin ii initiates sister chromatid resolution during s phase
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575537/
https://www.ncbi.nlm.nih.gov/pubmed/23401001
http://dx.doi.org/10.1083/jcb.201208008
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