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The chromatin remodeling factor Chd1l is required in the preimplantation embryo
During preimplantation development, the embryo must establish totipotency and enact the earliest differentiation choices, processes that involve extensive chromatin modification. To identify novel developmental regulators, we screened for genes that are preferentially transcribed in the pluripotent...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575647/ https://www.ncbi.nlm.nih.gov/pubmed/23429299 http://dx.doi.org/10.1242/bio.20122949 |
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author | Snider, Alyssa C. Leong, Denise Wang, Q. Tian Wysocka, Joanna Yao, Mylene W. M. Scott, Matthew P. |
author_facet | Snider, Alyssa C. Leong, Denise Wang, Q. Tian Wysocka, Joanna Yao, Mylene W. M. Scott, Matthew P. |
author_sort | Snider, Alyssa C. |
collection | PubMed |
description | During preimplantation development, the embryo must establish totipotency and enact the earliest differentiation choices, processes that involve extensive chromatin modification. To identify novel developmental regulators, we screened for genes that are preferentially transcribed in the pluripotent inner cell mass (ICM) of the mouse blastocyst. Genes that encode chromatin remodeling factors were prominently represented in the ICM, including Chd1l, a member of the Snf2 gene family. Chd1l is developmentally regulated and expressed in embryonic stem (ES) cells, but its role in development has not been investigated. Here we show that inhibiting Chd1l protein production by microinjection of antisense morpholinos causes arrest prior to the blastocyst stage. Despite this important function in vivo, Chd1l is non-essential for cultured ES cell survival, pluripotency, or differentiation, suggesting that Chd1l is vital for events in embryos that are distinct from events in ES cells. Our data reveal a novel role for the chromatin remodeling factor Chd1l in the earliest cell divisions of mammalian development. |
format | Online Article Text |
id | pubmed-3575647 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Company of Biologists |
record_format | MEDLINE/PubMed |
spelling | pubmed-35756472013-02-21 The chromatin remodeling factor Chd1l is required in the preimplantation embryo Snider, Alyssa C. Leong, Denise Wang, Q. Tian Wysocka, Joanna Yao, Mylene W. M. Scott, Matthew P. Biol Open Research Article During preimplantation development, the embryo must establish totipotency and enact the earliest differentiation choices, processes that involve extensive chromatin modification. To identify novel developmental regulators, we screened for genes that are preferentially transcribed in the pluripotent inner cell mass (ICM) of the mouse blastocyst. Genes that encode chromatin remodeling factors were prominently represented in the ICM, including Chd1l, a member of the Snf2 gene family. Chd1l is developmentally regulated and expressed in embryonic stem (ES) cells, but its role in development has not been investigated. Here we show that inhibiting Chd1l protein production by microinjection of antisense morpholinos causes arrest prior to the blastocyst stage. Despite this important function in vivo, Chd1l is non-essential for cultured ES cell survival, pluripotency, or differentiation, suggesting that Chd1l is vital for events in embryos that are distinct from events in ES cells. Our data reveal a novel role for the chromatin remodeling factor Chd1l in the earliest cell divisions of mammalian development. The Company of Biologists 2012-11-19 /pmc/articles/PMC3575647/ /pubmed/23429299 http://dx.doi.org/10.1242/bio.20122949 Text en © 2012. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by-nc-sa/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Article Snider, Alyssa C. Leong, Denise Wang, Q. Tian Wysocka, Joanna Yao, Mylene W. M. Scott, Matthew P. The chromatin remodeling factor Chd1l is required in the preimplantation embryo |
title | The chromatin remodeling factor Chd1l is required in the preimplantation embryo |
title_full | The chromatin remodeling factor Chd1l is required in the preimplantation embryo |
title_fullStr | The chromatin remodeling factor Chd1l is required in the preimplantation embryo |
title_full_unstemmed | The chromatin remodeling factor Chd1l is required in the preimplantation embryo |
title_short | The chromatin remodeling factor Chd1l is required in the preimplantation embryo |
title_sort | chromatin remodeling factor chd1l is required in the preimplantation embryo |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575647/ https://www.ncbi.nlm.nih.gov/pubmed/23429299 http://dx.doi.org/10.1242/bio.20122949 |
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