A novel non-radioactive primase–pyrophosphatase activity assay and its application to the discovery of inhibitors of Mycobacterium tuberculosis primase DnaG

Bacterial DNA primase DnaG synthesizes RNA primers required for chromosomal DNA replication. Biochemical assays measuring primase activity have been limited to monitoring formation of radioactively labelled primers because of the intrinsically low catalytic efficiency of DnaG. Furthermore, DnaG is p...

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Detalles Bibliográficos
Autores principales: Biswas, Tapan, Resto-Roldán, Esteban, Sawyer, Sean K., Artsimovitch, Irina, Tsodikov, Oleg V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575809/
https://www.ncbi.nlm.nih.gov/pubmed/23267008
http://dx.doi.org/10.1093/nar/gks1292

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575809/
https://www.ncbi.nlm.nih.gov/pubmed/23267008
http://dx.doi.org/10.1093/nar/gks1292

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