iSLIM: a comprehensive approach to mapping and characterizing gene regulatory networks

Mapping gene regulatory networks is a significant challenge in systems biology, yet only a few methods are currently capable of systems-level identification of transcription factors (TFs) that bind a specific regulatory element. We developed a microfluidic method for integrated systems-level interac...

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Autores principales: Rockel, Sylvie, Geertz, Marcel, Hens, Korneel, Deplancke, Bart, Maerkl, Sebastian J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575842/
https://www.ncbi.nlm.nih.gov/pubmed/23258699
http://dx.doi.org/10.1093/nar/gks1323
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author Rockel, Sylvie
Geertz, Marcel
Hens, Korneel
Deplancke, Bart
Maerkl, Sebastian J.
author_facet Rockel, Sylvie
Geertz, Marcel
Hens, Korneel
Deplancke, Bart
Maerkl, Sebastian J.
author_sort Rockel, Sylvie
collection PubMed
description Mapping gene regulatory networks is a significant challenge in systems biology, yet only a few methods are currently capable of systems-level identification of transcription factors (TFs) that bind a specific regulatory element. We developed a microfluidic method for integrated systems-level interaction mapping of TF–DNA interactions, generating and interrogating an array of 423 full-length Drosophila TFs. With integrated systems-level interaction mapping, it is now possible to rapidly and quantitatively map gene regulatory networks of higher eukaryotes.
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spelling pubmed-35758422013-02-19 iSLIM: a comprehensive approach to mapping and characterizing gene regulatory networks Rockel, Sylvie Geertz, Marcel Hens, Korneel Deplancke, Bart Maerkl, Sebastian J. Nucleic Acids Res Methods Online Mapping gene regulatory networks is a significant challenge in systems biology, yet only a few methods are currently capable of systems-level identification of transcription factors (TFs) that bind a specific regulatory element. We developed a microfluidic method for integrated systems-level interaction mapping of TF–DNA interactions, generating and interrogating an array of 423 full-length Drosophila TFs. With integrated systems-level interaction mapping, it is now possible to rapidly and quantitatively map gene regulatory networks of higher eukaryotes. Oxford University Press 2013-02 2012-12-18 /pmc/articles/PMC3575842/ /pubmed/23258699 http://dx.doi.org/10.1093/nar/gks1323 Text en © The Author(s) 2012. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.
spellingShingle Methods Online
Rockel, Sylvie
Geertz, Marcel
Hens, Korneel
Deplancke, Bart
Maerkl, Sebastian J.
iSLIM: a comprehensive approach to mapping and characterizing gene regulatory networks
title iSLIM: a comprehensive approach to mapping and characterizing gene regulatory networks
title_full iSLIM: a comprehensive approach to mapping and characterizing gene regulatory networks
title_fullStr iSLIM: a comprehensive approach to mapping and characterizing gene regulatory networks
title_full_unstemmed iSLIM: a comprehensive approach to mapping and characterizing gene regulatory networks
title_short iSLIM: a comprehensive approach to mapping and characterizing gene regulatory networks
title_sort islim: a comprehensive approach to mapping and characterizing gene regulatory networks
topic Methods Online
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575842/
https://www.ncbi.nlm.nih.gov/pubmed/23258699
http://dx.doi.org/10.1093/nar/gks1323
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