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The effect of hydroxycamptothecin and pingyangmycin on human squamous cell carcinoma of the tongue

The purpose of this study was to test hydroxycamptothecin (HCPT) and pingyangmycin (PYM) for their ability to inhibit the squamous cells of tongue carcinoma (Tca8113 cells). The effect of these compounds was tested using the MTT assay in vitro, clonogenic assays, flow cytometry, morphological observ...

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Detalles Bibliográficos
Autores principales: CHEN, PENG, LIU, BING, HU, MING
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3576210/
https://www.ncbi.nlm.nih.gov/pubmed/23426884
http://dx.doi.org/10.3892/ol.2013.1109
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author CHEN, PENG
LIU, BING
HU, MING
author_facet CHEN, PENG
LIU, BING
HU, MING
author_sort CHEN, PENG
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description The purpose of this study was to test hydroxycamptothecin (HCPT) and pingyangmycin (PYM) for their ability to inhibit the squamous cells of tongue carcinoma (Tca8113 cells). The effect of these compounds was tested using the MTT assay in vitro, clonogenic assays, flow cytometry, morphological observation, telomeric repeat amplification protocol (TRAP), transplantation of tumors into athymic mice and TUNEL staining. Treatment with HCPT and PYM, alone or in combination, inhibited the tumor cells and showed a greater inhibition when the drugs were combined. The cloning efficiency of Tca8113 cells was decreased. The microstructure and cell cycle of the cells changed significantly as a result of treatment. Telomerase activity was significantly inhibited in a time-dependent manner. By appearing to promote apoptosis, the drugs demonstrated a significant level of inhibition of the tumor cells in an athymic mouse model, promoting prolonged survival. HCPT and PYM have a marked cytotoxic effect on Tca8113 cells which is improved when used in combination.
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spelling pubmed-35762102013-02-20 The effect of hydroxycamptothecin and pingyangmycin on human squamous cell carcinoma of the tongue CHEN, PENG LIU, BING HU, MING Oncol Lett Articles The purpose of this study was to test hydroxycamptothecin (HCPT) and pingyangmycin (PYM) for their ability to inhibit the squamous cells of tongue carcinoma (Tca8113 cells). The effect of these compounds was tested using the MTT assay in vitro, clonogenic assays, flow cytometry, morphological observation, telomeric repeat amplification protocol (TRAP), transplantation of tumors into athymic mice and TUNEL staining. Treatment with HCPT and PYM, alone or in combination, inhibited the tumor cells and showed a greater inhibition when the drugs were combined. The cloning efficiency of Tca8113 cells was decreased. The microstructure and cell cycle of the cells changed significantly as a result of treatment. Telomerase activity was significantly inhibited in a time-dependent manner. By appearing to promote apoptosis, the drugs demonstrated a significant level of inhibition of the tumor cells in an athymic mouse model, promoting prolonged survival. HCPT and PYM have a marked cytotoxic effect on Tca8113 cells which is improved when used in combination. D.A. Spandidos 2013-03 2013-01-07 /pmc/articles/PMC3576210/ /pubmed/23426884 http://dx.doi.org/10.3892/ol.2013.1109 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
CHEN, PENG
LIU, BING
HU, MING
The effect of hydroxycamptothecin and pingyangmycin on human squamous cell carcinoma of the tongue
title The effect of hydroxycamptothecin and pingyangmycin on human squamous cell carcinoma of the tongue
title_full The effect of hydroxycamptothecin and pingyangmycin on human squamous cell carcinoma of the tongue
title_fullStr The effect of hydroxycamptothecin and pingyangmycin on human squamous cell carcinoma of the tongue
title_full_unstemmed The effect of hydroxycamptothecin and pingyangmycin on human squamous cell carcinoma of the tongue
title_short The effect of hydroxycamptothecin and pingyangmycin on human squamous cell carcinoma of the tongue
title_sort effect of hydroxycamptothecin and pingyangmycin on human squamous cell carcinoma of the tongue
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3576210/
https://www.ncbi.nlm.nih.gov/pubmed/23426884
http://dx.doi.org/10.3892/ol.2013.1109
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