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Identification of prostaglandin receptors in human ureters

BACKGROUND: Prostaglandins play an important role in ureteral obstruction, but the detailed expression profiles of the prostaglandin receptors (PTGER1, PTGER2, PTGER3, PTGER4, PTGFR) remain unknown in the different parts of the human ureter. METHODS: The expression pattern of PTGER1, PTGER2, PTGER3,...

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Detalles Bibliográficos
Autores principales: Oll, Matthias, Baumann, Claudia, Behbahani, Turang E, Ruecker, Alexander von, Müller, Stefan C, Ellinger, Jörg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3576244/
https://www.ncbi.nlm.nih.gov/pubmed/23227994
http://dx.doi.org/10.1186/1471-2490-12-35
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author Oll, Matthias
Baumann, Claudia
Behbahani, Turang E
Ruecker, Alexander von
Müller, Stefan C
Ellinger, Jörg
author_facet Oll, Matthias
Baumann, Claudia
Behbahani, Turang E
Ruecker, Alexander von
Müller, Stefan C
Ellinger, Jörg
author_sort Oll, Matthias
collection PubMed
description BACKGROUND: Prostaglandins play an important role in ureteral obstruction, but the detailed expression profiles of the prostaglandin receptors (PTGER1, PTGER2, PTGER3, PTGER4, PTGFR) remain unknown in the different parts of the human ureter. METHODS: The expression pattern of PTGER1, PTGER2, PTGER3, PTGER4 and PTGFR was determined in human distal, mid and proximal ureter and renal pelvis samples using immunohistochemistry (protein levels) and quantitative real-time PCR (mRNA). RESULTS: PTGER1 was highly expressed in most samples irrespective of the ureteral localization; however, urothelial cells had higher levels of PTGER1 than smooth muscle cells. PTGFR was also moderately to strongly expressed in urothelial and smooth muscle cells. In comparison, PTGER2-4 expression was mostly unexpressed or weakly expressed in urothelial and smooth cells in all regions. CONCLUSIONS: Our data indicate high levels of PTGER1 in ureters.
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spelling pubmed-35762442013-02-20 Identification of prostaglandin receptors in human ureters Oll, Matthias Baumann, Claudia Behbahani, Turang E Ruecker, Alexander von Müller, Stefan C Ellinger, Jörg BMC Urol Research Article BACKGROUND: Prostaglandins play an important role in ureteral obstruction, but the detailed expression profiles of the prostaglandin receptors (PTGER1, PTGER2, PTGER3, PTGER4, PTGFR) remain unknown in the different parts of the human ureter. METHODS: The expression pattern of PTGER1, PTGER2, PTGER3, PTGER4 and PTGFR was determined in human distal, mid and proximal ureter and renal pelvis samples using immunohistochemistry (protein levels) and quantitative real-time PCR (mRNA). RESULTS: PTGER1 was highly expressed in most samples irrespective of the ureteral localization; however, urothelial cells had higher levels of PTGER1 than smooth muscle cells. PTGFR was also moderately to strongly expressed in urothelial and smooth muscle cells. In comparison, PTGER2-4 expression was mostly unexpressed or weakly expressed in urothelial and smooth cells in all regions. CONCLUSIONS: Our data indicate high levels of PTGER1 in ureters. BioMed Central 2012-12-10 /pmc/articles/PMC3576244/ /pubmed/23227994 http://dx.doi.org/10.1186/1471-2490-12-35 Text en Copyright ©2012 Oll et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Oll, Matthias
Baumann, Claudia
Behbahani, Turang E
Ruecker, Alexander von
Müller, Stefan C
Ellinger, Jörg
Identification of prostaglandin receptors in human ureters
title Identification of prostaglandin receptors in human ureters
title_full Identification of prostaglandin receptors in human ureters
title_fullStr Identification of prostaglandin receptors in human ureters
title_full_unstemmed Identification of prostaglandin receptors in human ureters
title_short Identification of prostaglandin receptors in human ureters
title_sort identification of prostaglandin receptors in human ureters
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3576244/
https://www.ncbi.nlm.nih.gov/pubmed/23227994
http://dx.doi.org/10.1186/1471-2490-12-35
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