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Embelin-induced MCF-7 breast cancer cell apoptosis and blockade of MCF-7 cells in the G2/M phase via the mitochondrial pathway

Embelin is a small molecular inhibitor extracted from Myrsinaceae plants that specifically inhibits XIAP, affecting the proliferation and apoptosis of various types of tumor cells. In our previous studies, we have demonstrated that embelin is able to induce the apoptosis of MCF-7 breast cancer cells...

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Autores principales: LI, YANG, LI, DALEI, YUAN, SHENGGUANG, WANG, ZHENRAN, TANG, FANG, NIE, RONGRONG, WENG, JUN, MA, LINA, TANG, BO
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3576316/
https://www.ncbi.nlm.nih.gov/pubmed/23425971
http://dx.doi.org/10.3892/ol.2012.1084
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author LI, YANG
LI, DALEI
YUAN, SHENGGUANG
WANG, ZHENRAN
TANG, FANG
NIE, RONGRONG
WENG, JUN
MA, LINA
TANG, BO
author_facet LI, YANG
LI, DALEI
YUAN, SHENGGUANG
WANG, ZHENRAN
TANG, FANG
NIE, RONGRONG
WENG, JUN
MA, LINA
TANG, BO
author_sort LI, YANG
collection PubMed
description Embelin is a small molecular inhibitor extracted from Myrsinaceae plants that specifically inhibits XIAP, affecting the proliferation and apoptosis of various types of tumor cells. In our previous studies, we have demonstrated that embelin is able to induce the apoptosis of MCF-7 breast cancer cells in a dose-dependent manner. However, its mechanism of action is not yet clear. The purpose of this study was to investigate the involvement of the mitochondrial pathway in embelin-induced apoptosis and the effect of embelin on the cell cycle. Different doses of embelin were added to MCF-7 breast cancer cells and it was found that embelin was able to induce apoptosis of MCF-7 breast cancer cells in a dose- and time-dependent manner. Flow cytometry analysis revealed that embelin caused changes in the MCF-7 cell mitochondrial membrane potential and blocked the cell cycle of MCF-7 cells in the G2/M phase. Moreover, embelin was demonstrated to promote mitochondrial release of cytochrome C via regulation of Bax and Bcl-2, resulting in the activation of caspase-3 and -9, while no significant changes in the level of caspase-8 were observed. The results have demonstrated that embelin-induced apoptosis of MCF-7 breast cancer cells involves the mitochondrial pathway.
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spelling pubmed-35763162013-02-20 Embelin-induced MCF-7 breast cancer cell apoptosis and blockade of MCF-7 cells in the G2/M phase via the mitochondrial pathway LI, YANG LI, DALEI YUAN, SHENGGUANG WANG, ZHENRAN TANG, FANG NIE, RONGRONG WENG, JUN MA, LINA TANG, BO Oncol Lett Articles Embelin is a small molecular inhibitor extracted from Myrsinaceae plants that specifically inhibits XIAP, affecting the proliferation and apoptosis of various types of tumor cells. In our previous studies, we have demonstrated that embelin is able to induce the apoptosis of MCF-7 breast cancer cells in a dose-dependent manner. However, its mechanism of action is not yet clear. The purpose of this study was to investigate the involvement of the mitochondrial pathway in embelin-induced apoptosis and the effect of embelin on the cell cycle. Different doses of embelin were added to MCF-7 breast cancer cells and it was found that embelin was able to induce apoptosis of MCF-7 breast cancer cells in a dose- and time-dependent manner. Flow cytometry analysis revealed that embelin caused changes in the MCF-7 cell mitochondrial membrane potential and blocked the cell cycle of MCF-7 cells in the G2/M phase. Moreover, embelin was demonstrated to promote mitochondrial release of cytochrome C via regulation of Bax and Bcl-2, resulting in the activation of caspase-3 and -9, while no significant changes in the level of caspase-8 were observed. The results have demonstrated that embelin-induced apoptosis of MCF-7 breast cancer cells involves the mitochondrial pathway. D.A. Spandidos 2013-03 2012-12-19 /pmc/articles/PMC3576316/ /pubmed/23425971 http://dx.doi.org/10.3892/ol.2012.1084 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
LI, YANG
LI, DALEI
YUAN, SHENGGUANG
WANG, ZHENRAN
TANG, FANG
NIE, RONGRONG
WENG, JUN
MA, LINA
TANG, BO
Embelin-induced MCF-7 breast cancer cell apoptosis and blockade of MCF-7 cells in the G2/M phase via the mitochondrial pathway
title Embelin-induced MCF-7 breast cancer cell apoptosis and blockade of MCF-7 cells in the G2/M phase via the mitochondrial pathway
title_full Embelin-induced MCF-7 breast cancer cell apoptosis and blockade of MCF-7 cells in the G2/M phase via the mitochondrial pathway
title_fullStr Embelin-induced MCF-7 breast cancer cell apoptosis and blockade of MCF-7 cells in the G2/M phase via the mitochondrial pathway
title_full_unstemmed Embelin-induced MCF-7 breast cancer cell apoptosis and blockade of MCF-7 cells in the G2/M phase via the mitochondrial pathway
title_short Embelin-induced MCF-7 breast cancer cell apoptosis and blockade of MCF-7 cells in the G2/M phase via the mitochondrial pathway
title_sort embelin-induced mcf-7 breast cancer cell apoptosis and blockade of mcf-7 cells in the g2/m phase via the mitochondrial pathway
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3576316/
https://www.ncbi.nlm.nih.gov/pubmed/23425971
http://dx.doi.org/10.3892/ol.2012.1084
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