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Inhibition of cancer cell proliferation by midazolam by targeting transient receptor potential melastatin 7
Transient receptor potential melastatin 7 (TRPM7), a Ca(2+)-permeable channel, has been demonstrated to be present in cancer cells and involved in their growth and proliferation. The present study used midazolam, a benzodiazepine class anesthesic, to pharmacologically intervene in the expression of...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3576402/ https://www.ncbi.nlm.nih.gov/pubmed/23426784 http://dx.doi.org/10.3892/ol.2013.1129 |
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author | DOU, YUNLING LI, YUAN CHEN, JINGKAO WU, SIHAN XIAO, XIAO XIE, SHANSHAN TANG, LIPENG YAN, MIN WANG, YOUQIONG LIN, JUN ZHU, WENBO YAN, GUANGMEI |
author_facet | DOU, YUNLING LI, YUAN CHEN, JINGKAO WU, SIHAN XIAO, XIAO XIE, SHANSHAN TANG, LIPENG YAN, MIN WANG, YOUQIONG LIN, JUN ZHU, WENBO YAN, GUANGMEI |
author_sort | DOU, YUNLING |
collection | PubMed |
description | Transient receptor potential melastatin 7 (TRPM7), a Ca(2+)-permeable channel, has been demonstrated to be present in cancer cells and involved in their growth and proliferation. The present study used midazolam, a benzodiazepine class anesthesic, to pharmacologically intervene in the expression of TRPM7 and to inhibit cancer cell proliferation. Midazolam significantly inhibited the growth and proliferation of FaDu human hypopharyngeal squamous cell carcinoma cells, concurring with the induction of G(0)/G(1) cell cycle arrest and blockage of Rb activation. Central-type and peripheral-type benzodiazepine receptor antagonists did not abrogate proliferation inhibition by midazolam, while the specific TRPM7 agonist bradykinin reversed this effect. In addition, other benzodiazepines, diazepam and clonazepam also exhibited anti-proliferative activities. The inhibitory activity on cancer cell growth and proliferation, combined with the TRPM-dependent mechanism, reveals the anticancer potential of midazolam as a TRPM7 inhibitor and supports the suggestion that TRPM7 is a valuable target for pharmaceutical intervention. |
format | Online Article Text |
id | pubmed-3576402 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-35764022013-02-20 Inhibition of cancer cell proliferation by midazolam by targeting transient receptor potential melastatin 7 DOU, YUNLING LI, YUAN CHEN, JINGKAO WU, SIHAN XIAO, XIAO XIE, SHANSHAN TANG, LIPENG YAN, MIN WANG, YOUQIONG LIN, JUN ZHU, WENBO YAN, GUANGMEI Oncol Lett Articles Transient receptor potential melastatin 7 (TRPM7), a Ca(2+)-permeable channel, has been demonstrated to be present in cancer cells and involved in their growth and proliferation. The present study used midazolam, a benzodiazepine class anesthesic, to pharmacologically intervene in the expression of TRPM7 and to inhibit cancer cell proliferation. Midazolam significantly inhibited the growth and proliferation of FaDu human hypopharyngeal squamous cell carcinoma cells, concurring with the induction of G(0)/G(1) cell cycle arrest and blockage of Rb activation. Central-type and peripheral-type benzodiazepine receptor antagonists did not abrogate proliferation inhibition by midazolam, while the specific TRPM7 agonist bradykinin reversed this effect. In addition, other benzodiazepines, diazepam and clonazepam also exhibited anti-proliferative activities. The inhibitory activity on cancer cell growth and proliferation, combined with the TRPM-dependent mechanism, reveals the anticancer potential of midazolam as a TRPM7 inhibitor and supports the suggestion that TRPM7 is a valuable target for pharmaceutical intervention. D.A. Spandidos 2013-03 2013-01-11 /pmc/articles/PMC3576402/ /pubmed/23426784 http://dx.doi.org/10.3892/ol.2013.1129 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles DOU, YUNLING LI, YUAN CHEN, JINGKAO WU, SIHAN XIAO, XIAO XIE, SHANSHAN TANG, LIPENG YAN, MIN WANG, YOUQIONG LIN, JUN ZHU, WENBO YAN, GUANGMEI Inhibition of cancer cell proliferation by midazolam by targeting transient receptor potential melastatin 7 |
title | Inhibition of cancer cell proliferation by midazolam by targeting transient receptor potential melastatin 7 |
title_full | Inhibition of cancer cell proliferation by midazolam by targeting transient receptor potential melastatin 7 |
title_fullStr | Inhibition of cancer cell proliferation by midazolam by targeting transient receptor potential melastatin 7 |
title_full_unstemmed | Inhibition of cancer cell proliferation by midazolam by targeting transient receptor potential melastatin 7 |
title_short | Inhibition of cancer cell proliferation by midazolam by targeting transient receptor potential melastatin 7 |
title_sort | inhibition of cancer cell proliferation by midazolam by targeting transient receptor potential melastatin 7 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3576402/ https://www.ncbi.nlm.nih.gov/pubmed/23426784 http://dx.doi.org/10.3892/ol.2013.1129 |
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