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Involvement of spinal orexin A in the electroacupuncture analgesia in a rat model of post-laparotomy pain
BACKGROUND: Orexin A (OXA, hypocretin/hcrt 1) is a newly discovered potential analgesic substance. However, whether OXA is involved in acupuncture analgesia remains unknown. The present study was designed to investigate the involvement of spinal OXA in electroacupuncture (EA) analgesia. METHODS: A m...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3577429/ https://www.ncbi.nlm.nih.gov/pubmed/23173601 http://dx.doi.org/10.1186/1472-6882-12-225 |
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author | Feng, Xiao-Ming Mi, Wen-Li Xia, Fang Mao-Ying, Qi-Liang Jiang, Jian-Wei Xiao, Sheng Wang, Zhi-Fu Wang, Yan-Qing Wu, Gen-Cheng |
author_facet | Feng, Xiao-Ming Mi, Wen-Li Xia, Fang Mao-Ying, Qi-Liang Jiang, Jian-Wei Xiao, Sheng Wang, Zhi-Fu Wang, Yan-Qing Wu, Gen-Cheng |
author_sort | Feng, Xiao-Ming |
collection | PubMed |
description | BACKGROUND: Orexin A (OXA, hypocretin/hcrt 1) is a newly discovered potential analgesic substance. However, whether OXA is involved in acupuncture analgesia remains unknown. The present study was designed to investigate the involvement of spinal OXA in electroacupuncture (EA) analgesia. METHODS: A modified rat model of post-laparotomy pain was adopted and evaluated. Von Frey filaments were used to measure mechanical allodynia of the hind paw and abdomen. EA at 2/15 Hz or 2/100 Hz was performed once on the bilateral ST36 and SP6 for 30 min perioperatively. SB-334867, a selective orexin 1 receptor (OX1R) antagonist with a higher affinity for OXA than OXB, was intrathecally injected to observe its effect on EA analgesia. RESULTS: OXA at 0.3 nmol and EA at 2/15 Hz produced respective analgesic effects on the model (P<0.05). Pre-surgical intrathecal administered of SB-334867 30 nmol antagonized OXA analgesia and attenuated the analgesic effect of EA (P<0.05). However, SB-334867 did not block fentanyl-induced analgesia (P>0.05). In addition, naloxone, a selective opioid receptor antagonist, failed to antagonize OXA-induced analgesia (P>0.05). CONCLUSIONS: The results of the present study indicate the involvement of OXA in EA analgesia via OX1R in an opioid-independent way. |
format | Online Article Text |
id | pubmed-3577429 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35774292013-02-21 Involvement of spinal orexin A in the electroacupuncture analgesia in a rat model of post-laparotomy pain Feng, Xiao-Ming Mi, Wen-Li Xia, Fang Mao-Ying, Qi-Liang Jiang, Jian-Wei Xiao, Sheng Wang, Zhi-Fu Wang, Yan-Qing Wu, Gen-Cheng BMC Complement Altern Med Research Article BACKGROUND: Orexin A (OXA, hypocretin/hcrt 1) is a newly discovered potential analgesic substance. However, whether OXA is involved in acupuncture analgesia remains unknown. The present study was designed to investigate the involvement of spinal OXA in electroacupuncture (EA) analgesia. METHODS: A modified rat model of post-laparotomy pain was adopted and evaluated. Von Frey filaments were used to measure mechanical allodynia of the hind paw and abdomen. EA at 2/15 Hz or 2/100 Hz was performed once on the bilateral ST36 and SP6 for 30 min perioperatively. SB-334867, a selective orexin 1 receptor (OX1R) antagonist with a higher affinity for OXA than OXB, was intrathecally injected to observe its effect on EA analgesia. RESULTS: OXA at 0.3 nmol and EA at 2/15 Hz produced respective analgesic effects on the model (P<0.05). Pre-surgical intrathecal administered of SB-334867 30 nmol antagonized OXA analgesia and attenuated the analgesic effect of EA (P<0.05). However, SB-334867 did not block fentanyl-induced analgesia (P>0.05). In addition, naloxone, a selective opioid receptor antagonist, failed to antagonize OXA-induced analgesia (P>0.05). CONCLUSIONS: The results of the present study indicate the involvement of OXA in EA analgesia via OX1R in an opioid-independent way. BioMed Central 2012-11-22 /pmc/articles/PMC3577429/ /pubmed/23173601 http://dx.doi.org/10.1186/1472-6882-12-225 Text en Copyright ©2012 Feng et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Feng, Xiao-Ming Mi, Wen-Li Xia, Fang Mao-Ying, Qi-Liang Jiang, Jian-Wei Xiao, Sheng Wang, Zhi-Fu Wang, Yan-Qing Wu, Gen-Cheng Involvement of spinal orexin A in the electroacupuncture analgesia in a rat model of post-laparotomy pain |
title | Involvement of spinal orexin A in the electroacupuncture analgesia in a rat model of post-laparotomy pain |
title_full | Involvement of spinal orexin A in the electroacupuncture analgesia in a rat model of post-laparotomy pain |
title_fullStr | Involvement of spinal orexin A in the electroacupuncture analgesia in a rat model of post-laparotomy pain |
title_full_unstemmed | Involvement of spinal orexin A in the electroacupuncture analgesia in a rat model of post-laparotomy pain |
title_short | Involvement of spinal orexin A in the electroacupuncture analgesia in a rat model of post-laparotomy pain |
title_sort | involvement of spinal orexin a in the electroacupuncture analgesia in a rat model of post-laparotomy pain |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3577429/ https://www.ncbi.nlm.nih.gov/pubmed/23173601 http://dx.doi.org/10.1186/1472-6882-12-225 |
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