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Dietary oil composition differentially modulates intestinal endotoxin transport and postprandial endotoxemia

BACKGROUND: Intestinal derived endotoxin and the subsequent endotoxemia can be considered major predisposing factors for diseases such as atherosclerosis, sepsis, obesity and diabetes. Dietary fat has been shown to increase postprandial endotoxemia. Therefore, the aim of this study was to assess the...

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Autores principales: Mani, Venkatesh, Hollis, James H, Gabler, Nicholas K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3577458/
https://www.ncbi.nlm.nih.gov/pubmed/23305038
http://dx.doi.org/10.1186/1743-7075-10-6
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author Mani, Venkatesh
Hollis, James H
Gabler, Nicholas K
author_facet Mani, Venkatesh
Hollis, James H
Gabler, Nicholas K
author_sort Mani, Venkatesh
collection PubMed
description BACKGROUND: Intestinal derived endotoxin and the subsequent endotoxemia can be considered major predisposing factors for diseases such as atherosclerosis, sepsis, obesity and diabetes. Dietary fat has been shown to increase postprandial endotoxemia. Therefore, the aim of this study was to assess the effects of different dietary oils on intestinal endotoxin transport and postprandial endotoxemia using swine as a model. We hypothesized that oils rich in saturated fatty acids (SFA) would augment, while oils rich in n-3 polyunsaturated fatty acids (PUFA) would attenuate intestinal endotoxin transport and circulating concentrations. METHODS: Postprandial endotoxemia was measured in twenty four pigs following a porridge meal made with either water (Control), fish oil (FO), vegetable oil (VO) or coconut oil (CO). Blood was collected at 0, 1, 2, 3 and 5 hours postprandial and measured for endotoxin. Furthermore, ex vivo ileum endotoxin transport was assessed using modified Ussing chambers and intestines were treated with either no oil or 12.5% (v/v) VO, FO, cod liver oil (CLO), CO or olive oil (OO). Ex vivo mucosal to serosal endotoxin transport permeability (Papp) was then measured by the addition of fluorescent labeled-lipopolysaccharide. RESULTS: Postprandial serum endotoxin concentrations were increased after a meal rich in saturated fatty acids and decreased with higher n-3 PUFA intake. Compared to the no oil control, fish oil and CLO which are rich in n-3 fatty acids reduced ex vivo endotoxin Papp by 50% (P < 0.05). Contrarily, saturated fatty acids increased the Papp by 60% (P = 0.008). Olive and vegetable oils did not alter intestinal endotoxin Papp. CONCLUSION: Overall, these results indicate that saturated and n-3 PUFA differentially regulate intestinal epithelial endotoxin transport. This may be associated with fatty acid regulation of intestinal membrane lipid raft mediated permeability.
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spelling pubmed-35774582013-02-26 Dietary oil composition differentially modulates intestinal endotoxin transport and postprandial endotoxemia Mani, Venkatesh Hollis, James H Gabler, Nicholas K Nutr Metab (Lond) Research BACKGROUND: Intestinal derived endotoxin and the subsequent endotoxemia can be considered major predisposing factors for diseases such as atherosclerosis, sepsis, obesity and diabetes. Dietary fat has been shown to increase postprandial endotoxemia. Therefore, the aim of this study was to assess the effects of different dietary oils on intestinal endotoxin transport and postprandial endotoxemia using swine as a model. We hypothesized that oils rich in saturated fatty acids (SFA) would augment, while oils rich in n-3 polyunsaturated fatty acids (PUFA) would attenuate intestinal endotoxin transport and circulating concentrations. METHODS: Postprandial endotoxemia was measured in twenty four pigs following a porridge meal made with either water (Control), fish oil (FO), vegetable oil (VO) or coconut oil (CO). Blood was collected at 0, 1, 2, 3 and 5 hours postprandial and measured for endotoxin. Furthermore, ex vivo ileum endotoxin transport was assessed using modified Ussing chambers and intestines were treated with either no oil or 12.5% (v/v) VO, FO, cod liver oil (CLO), CO or olive oil (OO). Ex vivo mucosal to serosal endotoxin transport permeability (Papp) was then measured by the addition of fluorescent labeled-lipopolysaccharide. RESULTS: Postprandial serum endotoxin concentrations were increased after a meal rich in saturated fatty acids and decreased with higher n-3 PUFA intake. Compared to the no oil control, fish oil and CLO which are rich in n-3 fatty acids reduced ex vivo endotoxin Papp by 50% (P < 0.05). Contrarily, saturated fatty acids increased the Papp by 60% (P = 0.008). Olive and vegetable oils did not alter intestinal endotoxin Papp. CONCLUSION: Overall, these results indicate that saturated and n-3 PUFA differentially regulate intestinal epithelial endotoxin transport. This may be associated with fatty acid regulation of intestinal membrane lipid raft mediated permeability. BioMed Central 2013-01-10 /pmc/articles/PMC3577458/ /pubmed/23305038 http://dx.doi.org/10.1186/1743-7075-10-6 Text en Copyright ©2013 Mani et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Mani, Venkatesh
Hollis, James H
Gabler, Nicholas K
Dietary oil composition differentially modulates intestinal endotoxin transport and postprandial endotoxemia
title Dietary oil composition differentially modulates intestinal endotoxin transport and postprandial endotoxemia
title_full Dietary oil composition differentially modulates intestinal endotoxin transport and postprandial endotoxemia
title_fullStr Dietary oil composition differentially modulates intestinal endotoxin transport and postprandial endotoxemia
title_full_unstemmed Dietary oil composition differentially modulates intestinal endotoxin transport and postprandial endotoxemia
title_short Dietary oil composition differentially modulates intestinal endotoxin transport and postprandial endotoxemia
title_sort dietary oil composition differentially modulates intestinal endotoxin transport and postprandial endotoxemia
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3577458/
https://www.ncbi.nlm.nih.gov/pubmed/23305038
http://dx.doi.org/10.1186/1743-7075-10-6
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