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A new approach for aggregation of Paramecium caudatum by nitric oxide

BACKGROUND AND OBJECTIVES: Nitric oxide (NO) plays a role in thermoregulation and growth of protozoa. This work aimed to add the molecule NO in physiology of protozoa in contact with abused narcotic substances. MATERIALS AND METHODS: A sedative drug, morphine, was infused into a cell chamber contain...

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Detalles Bibliográficos
Autores principales: Karami, Manizheh, Shahrokhi, Seyed Sajad, Kazemi, Bahram, Moezzi, Seyedeh Samaneh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3577564/
https://www.ncbi.nlm.nih.gov/pubmed/23467065
Descripción
Sumario:BACKGROUND AND OBJECTIVES: Nitric oxide (NO) plays a role in thermoregulation and growth of protozoa. This work aimed to add the molecule NO in physiology of protozoa in contact with abused narcotic substances. MATERIALS AND METHODS: A sedative drug, morphine, was infused into a cell chamber containing Paramecia. The cell response to the drug was recorded promptly after drug infusion using a potency protocol provided for the first time at this laboratory. A precursor of NO, L-arginine, was treated jointly with drug to involve the NO system in protozoan performance to drug exposure. Marking of NADPH-diaphorase (NADPH-d) was followed to provide data to explain the mechanisms. RESULTS: Morphine particularly (0.5 to 60 µg/µ1) aggregated the Paramecia. The infusion of L-arginine (1 to 8 µg/µ1) together with morphine potentiated this effect, though, pre-usage of L-NAME (1 to 8 µg/µ1), a blocker of NO production, reversed the response. Notably the activation of NADPH-d in solely morphine or L-arginine plus morphine samples was revealed. However, the expression of marker was attenuated upon pre-infusion with L-NAME. CONCLUSION: This study introduces a new approach to involve NO in physiology of aggregation of Paramecia following exposure to the misused sedative drug, morphine.