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A new approach for aggregation of Paramecium caudatum by nitric oxide
BACKGROUND AND OBJECTIVES: Nitric oxide (NO) plays a role in thermoregulation and growth of protozoa. This work aimed to add the molecule NO in physiology of protozoa in contact with abused narcotic substances. MATERIALS AND METHODS: A sedative drug, morphine, was infused into a cell chamber contain...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tehran University of Medical Sciences
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3577564/ https://www.ncbi.nlm.nih.gov/pubmed/23467065 |
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author | Karami, Manizheh Shahrokhi, Seyed Sajad Kazemi, Bahram Moezzi, Seyedeh Samaneh |
author_facet | Karami, Manizheh Shahrokhi, Seyed Sajad Kazemi, Bahram Moezzi, Seyedeh Samaneh |
author_sort | Karami, Manizheh |
collection | PubMed |
description | BACKGROUND AND OBJECTIVES: Nitric oxide (NO) plays a role in thermoregulation and growth of protozoa. This work aimed to add the molecule NO in physiology of protozoa in contact with abused narcotic substances. MATERIALS AND METHODS: A sedative drug, morphine, was infused into a cell chamber containing Paramecia. The cell response to the drug was recorded promptly after drug infusion using a potency protocol provided for the first time at this laboratory. A precursor of NO, L-arginine, was treated jointly with drug to involve the NO system in protozoan performance to drug exposure. Marking of NADPH-diaphorase (NADPH-d) was followed to provide data to explain the mechanisms. RESULTS: Morphine particularly (0.5 to 60 µg/µ1) aggregated the Paramecia. The infusion of L-arginine (1 to 8 µg/µ1) together with morphine potentiated this effect, though, pre-usage of L-NAME (1 to 8 µg/µ1), a blocker of NO production, reversed the response. Notably the activation of NADPH-d in solely morphine or L-arginine plus morphine samples was revealed. However, the expression of marker was attenuated upon pre-infusion with L-NAME. CONCLUSION: This study introduces a new approach to involve NO in physiology of aggregation of Paramecia following exposure to the misused sedative drug, morphine. |
format | Online Article Text |
id | pubmed-3577564 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-35775642013-03-06 A new approach for aggregation of Paramecium caudatum by nitric oxide Karami, Manizheh Shahrokhi, Seyed Sajad Kazemi, Bahram Moezzi, Seyedeh Samaneh Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: Nitric oxide (NO) plays a role in thermoregulation and growth of protozoa. This work aimed to add the molecule NO in physiology of protozoa in contact with abused narcotic substances. MATERIALS AND METHODS: A sedative drug, morphine, was infused into a cell chamber containing Paramecia. The cell response to the drug was recorded promptly after drug infusion using a potency protocol provided for the first time at this laboratory. A precursor of NO, L-arginine, was treated jointly with drug to involve the NO system in protozoan performance to drug exposure. Marking of NADPH-diaphorase (NADPH-d) was followed to provide data to explain the mechanisms. RESULTS: Morphine particularly (0.5 to 60 µg/µ1) aggregated the Paramecia. The infusion of L-arginine (1 to 8 µg/µ1) together with morphine potentiated this effect, though, pre-usage of L-NAME (1 to 8 µg/µ1), a blocker of NO production, reversed the response. Notably the activation of NADPH-d in solely morphine or L-arginine plus morphine samples was revealed. However, the expression of marker was attenuated upon pre-infusion with L-NAME. CONCLUSION: This study introduces a new approach to involve NO in physiology of aggregation of Paramecia following exposure to the misused sedative drug, morphine. Tehran University of Medical Sciences 2013-03 /pmc/articles/PMC3577564/ /pubmed/23467065 Text en © 2013 Iranian Society of Microbiology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Karami, Manizheh Shahrokhi, Seyed Sajad Kazemi, Bahram Moezzi, Seyedeh Samaneh A new approach for aggregation of Paramecium caudatum by nitric oxide |
title | A new approach for aggregation of Paramecium caudatum by nitric oxide |
title_full | A new approach for aggregation of Paramecium caudatum by nitric oxide |
title_fullStr | A new approach for aggregation of Paramecium caudatum by nitric oxide |
title_full_unstemmed | A new approach for aggregation of Paramecium caudatum by nitric oxide |
title_short | A new approach for aggregation of Paramecium caudatum by nitric oxide |
title_sort | new approach for aggregation of paramecium caudatum by nitric oxide |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3577564/ https://www.ncbi.nlm.nih.gov/pubmed/23467065 |
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