Immune reactivity of Brucella melitensis–vaccinated rabbit serum with recombinant Omp31 and DnaK proteins

BACKGROUND AND OBJECTIVES: Brucella melitensis infection is still a major health problem for human and cattle in developing countries and the Middle East. MATERIALS AND METHODS: In this study, in order to screen immunogenic candidate antigens for the development of a Brucella subunit vaccine, a cyto...

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Detalles Bibliográficos
Autores principales: Ghasemi, Amir, Salari, Mohammad Hossein, Zarnani, Amir Hassan, Pourmand, Mohammad Reza, Ahmadi, Hojat, Mirshafiey, Abbas, Jeddi-Tehrani, Mahmood
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2013
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3577567/
https://www.ncbi.nlm.nih.gov/pubmed/23467315
Descripción
Sumario:BACKGROUND AND OBJECTIVES: Brucella melitensis infection is still a major health problem for human and cattle in developing countries and the Middle East. MATERIALS AND METHODS: In this study, in order to screen immunogenic candidate antigens for the development of a Brucella subunit vaccine, a cytoplasmic protein (DnaK) and an outer membrane protein (Omp31) of B. melitensis were cloned, expressed in E.coli BL21 and then purified using Ni-NTA agarose. Immunized serum was prepared from a rabbit inoculated with attenuated B. melitensis. RESULTS AND CONCLUSION: It was proved that immunized serum contains antibodies against recombinant Omp31 (rOmp31) and DnaK (rDnaK) by Western blot and ELISA assays. The results may suggest the importance of these proteins as subunit vaccines against B. melitensis as well as targets for immunotherapy.

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