Immune reactivity of Brucella melitensis–vaccinated rabbit serum with recombinant Omp31 and DnaK proteins

BACKGROUND AND OBJECTIVES: Brucella melitensis infection is still a major health problem for human and cattle in developing countries and the Middle East. MATERIALS AND METHODS: In this study, in order to screen immunogenic candidate antigens for the development of a Brucella subunit vaccine, a cyto...

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Autores principales: Ghasemi, Amir, Salari, Mohammad Hossein, Zarnani, Amir Hassan, Pourmand, Mohammad Reza, Ahmadi, Hojat, Mirshafiey, Abbas, Jeddi-Tehrani, Mahmood
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2013
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3577567/
https://www.ncbi.nlm.nih.gov/pubmed/23467315
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author Ghasemi, Amir
Salari, Mohammad Hossein
Zarnani, Amir Hassan
Pourmand, Mohammad Reza
Ahmadi, Hojat
Mirshafiey, Abbas
Jeddi-Tehrani, Mahmood
author_facet Ghasemi, Amir
Salari, Mohammad Hossein
Zarnani, Amir Hassan
Pourmand, Mohammad Reza
Ahmadi, Hojat
Mirshafiey, Abbas
Jeddi-Tehrani, Mahmood
author_sort Ghasemi, Amir
collection PubMed
description BACKGROUND AND OBJECTIVES: Brucella melitensis infection is still a major health problem for human and cattle in developing countries and the Middle East. MATERIALS AND METHODS: In this study, in order to screen immunogenic candidate antigens for the development of a Brucella subunit vaccine, a cytoplasmic protein (DnaK) and an outer membrane protein (Omp31) of B. melitensis were cloned, expressed in E.coli BL21 and then purified using Ni-NTA agarose. Immunized serum was prepared from a rabbit inoculated with attenuated B. melitensis. RESULTS AND CONCLUSION: It was proved that immunized serum contains antibodies against recombinant Omp31 (rOmp31) and DnaK (rDnaK) by Western blot and ELISA assays. The results may suggest the importance of these proteins as subunit vaccines against B. melitensis as well as targets for immunotherapy.
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spelling pubmed-35775672013-03-06 Immune reactivity of Brucella melitensis–vaccinated rabbit serum with recombinant Omp31 and DnaK proteins Ghasemi, Amir Salari, Mohammad Hossein Zarnani, Amir Hassan Pourmand, Mohammad Reza Ahmadi, Hojat Mirshafiey, Abbas Jeddi-Tehrani, Mahmood Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: Brucella melitensis infection is still a major health problem for human and cattle in developing countries and the Middle East. MATERIALS AND METHODS: In this study, in order to screen immunogenic candidate antigens for the development of a Brucella subunit vaccine, a cytoplasmic protein (DnaK) and an outer membrane protein (Omp31) of B. melitensis were cloned, expressed in E.coli BL21 and then purified using Ni-NTA agarose. Immunized serum was prepared from a rabbit inoculated with attenuated B. melitensis. RESULTS AND CONCLUSION: It was proved that immunized serum contains antibodies against recombinant Omp31 (rOmp31) and DnaK (rDnaK) by Western blot and ELISA assays. The results may suggest the importance of these proteins as subunit vaccines against B. melitensis as well as targets for immunotherapy. Tehran University of Medical Sciences 2013-03 /pmc/articles/PMC3577567/ /pubmed/23467315 Text en © 2013 Iranian Society of Microbiology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Ghasemi, Amir
Salari, Mohammad Hossein
Zarnani, Amir Hassan
Pourmand, Mohammad Reza
Ahmadi, Hojat
Mirshafiey, Abbas
Jeddi-Tehrani, Mahmood
Immune reactivity of Brucella melitensis–vaccinated rabbit serum with recombinant Omp31 and DnaK proteins
title Immune reactivity of Brucella melitensis–vaccinated rabbit serum with recombinant Omp31 and DnaK proteins
title_full Immune reactivity of Brucella melitensis–vaccinated rabbit serum with recombinant Omp31 and DnaK proteins
title_fullStr Immune reactivity of Brucella melitensis–vaccinated rabbit serum with recombinant Omp31 and DnaK proteins
title_full_unstemmed Immune reactivity of Brucella melitensis–vaccinated rabbit serum with recombinant Omp31 and DnaK proteins
title_short Immune reactivity of Brucella melitensis–vaccinated rabbit serum with recombinant Omp31 and DnaK proteins
title_sort immune reactivity of brucella melitensis–vaccinated rabbit serum with recombinant omp31 and dnak proteins
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3577567/
https://www.ncbi.nlm.nih.gov/pubmed/23467315
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