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High-Fidelity Tissue Engineering of Patient-Specific Auricles for Reconstruction of Pediatric Microtia and Other Auricular Deformities

INTRODUCTION: Autologous techniques for the reconstruction of pediatric microtia often result in suboptimal aesthetic outcomes and morbidity at the costal cartilage donor site. We therefore sought to combine digital photogrammetry with CAD/CAM techniques to develop collagen type I hydrogel scaffolds...

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Autores principales: Reiffel, Alyssa J., Kafka, Concepcion, Hernandez, Karina A., Popa, Samantha, Perez, Justin L., Zhou, Sherry, Pramanik, Satadru, Brown, Bryan N., Ryu, Won Seuk, Bonassar, Lawrence J., Spector, Jason A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3577892/
https://www.ncbi.nlm.nih.gov/pubmed/23437148
http://dx.doi.org/10.1371/journal.pone.0056506
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author Reiffel, Alyssa J.
Kafka, Concepcion
Hernandez, Karina A.
Popa, Samantha
Perez, Justin L.
Zhou, Sherry
Pramanik, Satadru
Brown, Bryan N.
Ryu, Won Seuk
Bonassar, Lawrence J.
Spector, Jason A.
author_facet Reiffel, Alyssa J.
Kafka, Concepcion
Hernandez, Karina A.
Popa, Samantha
Perez, Justin L.
Zhou, Sherry
Pramanik, Satadru
Brown, Bryan N.
Ryu, Won Seuk
Bonassar, Lawrence J.
Spector, Jason A.
author_sort Reiffel, Alyssa J.
collection PubMed
description INTRODUCTION: Autologous techniques for the reconstruction of pediatric microtia often result in suboptimal aesthetic outcomes and morbidity at the costal cartilage donor site. We therefore sought to combine digital photogrammetry with CAD/CAM techniques to develop collagen type I hydrogel scaffolds and their respective molds that would precisely mimic the normal anatomy of the patient-specific external ear as well as recapitulate the complex biomechanical properties of native auricular elastic cartilage while avoiding the morbidity of traditional autologous reconstructions. METHODS: Three-dimensional structures of normal pediatric ears were digitized and converted to virtual solids for mold design. Image-based synthetic reconstructions of these ears were fabricated from collagen type I hydrogels. Half were seeded with bovine auricular chondrocytes. Cellular and acellular constructs were implanted subcutaneously in the dorsa of nude rats and harvested after 1 and 3 months. RESULTS: Gross inspection revealed that acellular implants had significantly decreased in size by 1 month. Cellular constructs retained their contour/projection from the animals' dorsa, even after 3 months. Post-harvest weight of cellular constructs was significantly greater than that of acellular constructs after 1 and 3 months. Safranin O-staining revealed that cellular constructs demonstrated evidence of a self-assembled perichondrial layer and copious neocartilage deposition. Verhoeff staining of 1 month cellular constructs revealed de novo elastic cartilage deposition, which was even more extensive and robust after 3 months. The equilibrium modulus and hydraulic permeability of cellular constructs were not significantly different from native bovine auricular cartilage after 3 months. CONCLUSIONS: We have developed high-fidelity, biocompatible, patient-specific tissue-engineered constructs for auricular reconstruction which largely mimic the native auricle both biomechanically and histologically, even after an extended period of implantation. This strategy holds immense potential for durable patient-specific tissue-engineered anatomically proper auricular reconstructions in the future.
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spelling pubmed-35778922013-02-22 High-Fidelity Tissue Engineering of Patient-Specific Auricles for Reconstruction of Pediatric Microtia and Other Auricular Deformities Reiffel, Alyssa J. Kafka, Concepcion Hernandez, Karina A. Popa, Samantha Perez, Justin L. Zhou, Sherry Pramanik, Satadru Brown, Bryan N. Ryu, Won Seuk Bonassar, Lawrence J. Spector, Jason A. PLoS One Research Article INTRODUCTION: Autologous techniques for the reconstruction of pediatric microtia often result in suboptimal aesthetic outcomes and morbidity at the costal cartilage donor site. We therefore sought to combine digital photogrammetry with CAD/CAM techniques to develop collagen type I hydrogel scaffolds and their respective molds that would precisely mimic the normal anatomy of the patient-specific external ear as well as recapitulate the complex biomechanical properties of native auricular elastic cartilage while avoiding the morbidity of traditional autologous reconstructions. METHODS: Three-dimensional structures of normal pediatric ears were digitized and converted to virtual solids for mold design. Image-based synthetic reconstructions of these ears were fabricated from collagen type I hydrogels. Half were seeded with bovine auricular chondrocytes. Cellular and acellular constructs were implanted subcutaneously in the dorsa of nude rats and harvested after 1 and 3 months. RESULTS: Gross inspection revealed that acellular implants had significantly decreased in size by 1 month. Cellular constructs retained their contour/projection from the animals' dorsa, even after 3 months. Post-harvest weight of cellular constructs was significantly greater than that of acellular constructs after 1 and 3 months. Safranin O-staining revealed that cellular constructs demonstrated evidence of a self-assembled perichondrial layer and copious neocartilage deposition. Verhoeff staining of 1 month cellular constructs revealed de novo elastic cartilage deposition, which was even more extensive and robust after 3 months. The equilibrium modulus and hydraulic permeability of cellular constructs were not significantly different from native bovine auricular cartilage after 3 months. CONCLUSIONS: We have developed high-fidelity, biocompatible, patient-specific tissue-engineered constructs for auricular reconstruction which largely mimic the native auricle both biomechanically and histologically, even after an extended period of implantation. This strategy holds immense potential for durable patient-specific tissue-engineered anatomically proper auricular reconstructions in the future. Public Library of Science 2013-02-20 /pmc/articles/PMC3577892/ /pubmed/23437148 http://dx.doi.org/10.1371/journal.pone.0056506 Text en © 2013 Reiffel et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Reiffel, Alyssa J.
Kafka, Concepcion
Hernandez, Karina A.
Popa, Samantha
Perez, Justin L.
Zhou, Sherry
Pramanik, Satadru
Brown, Bryan N.
Ryu, Won Seuk
Bonassar, Lawrence J.
Spector, Jason A.
High-Fidelity Tissue Engineering of Patient-Specific Auricles for Reconstruction of Pediatric Microtia and Other Auricular Deformities
title High-Fidelity Tissue Engineering of Patient-Specific Auricles for Reconstruction of Pediatric Microtia and Other Auricular Deformities
title_full High-Fidelity Tissue Engineering of Patient-Specific Auricles for Reconstruction of Pediatric Microtia and Other Auricular Deformities
title_fullStr High-Fidelity Tissue Engineering of Patient-Specific Auricles for Reconstruction of Pediatric Microtia and Other Auricular Deformities
title_full_unstemmed High-Fidelity Tissue Engineering of Patient-Specific Auricles for Reconstruction of Pediatric Microtia and Other Auricular Deformities
title_short High-Fidelity Tissue Engineering of Patient-Specific Auricles for Reconstruction of Pediatric Microtia and Other Auricular Deformities
title_sort high-fidelity tissue engineering of patient-specific auricles for reconstruction of pediatric microtia and other auricular deformities
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3577892/
https://www.ncbi.nlm.nih.gov/pubmed/23437148
http://dx.doi.org/10.1371/journal.pone.0056506
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