Optogenetic inhibition of cocaine seeking in rats

Inhibitory optogenetics was used to examine the roles of the prelimbic cortex (PL), the nucleus accumbens core (NAcore) and the PL projections to the NAcore in the reinstatement of cocaine seeking. Rats were microinjected into the PL or NAcore with an adeno-associated virus containing halorhodopsin...

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Autores principales: Stefanik, Michael T, Moussawi, Khaled, Kupchik, Yonatan M, Smith, Kyle C, Miller, Rachel L, Huff, Mary L, Deisseroth, Karl, Kalivas, Peter W, LaLumiere, Ryan T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2013
Materias:
Preclinical Studies
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3578202/
https://www.ncbi.nlm.nih.gov/pubmed/22823160
http://dx.doi.org/10.1111/j.1369-1600.2012.00479.x
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author Stefanik, Michael T
Moussawi, Khaled
Kupchik, Yonatan M
Smith, Kyle C
Miller, Rachel L
Huff, Mary L
Deisseroth, Karl
Kalivas, Peter W
LaLumiere, Ryan T
author_facet Stefanik, Michael T
Moussawi, Khaled
Kupchik, Yonatan M
Smith, Kyle C
Miller, Rachel L
Huff, Mary L
Deisseroth, Karl
Kalivas, Peter W
LaLumiere, Ryan T
author_sort Stefanik, Michael T
collection PubMed
description Inhibitory optogenetics was used to examine the roles of the prelimbic cortex (PL), the nucleus accumbens core (NAcore) and the PL projections to the NAcore in the reinstatement of cocaine seeking. Rats were microinjected into the PL or NAcore with an adeno-associated virus containing halorhodopsin or archaerhodopsin. After 12 days of cocaine self-administration, followed by extinction training, animals underwent reinstatement testing along with the presence/absence of optically induced inhibition via laser light. Bilateral optical inhibition of the PL, NAcore or the PL fibers in the NAcore inhibited the reinstatement of cocaine seeking.
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spelling pubmed-35782022013-10-16 Optogenetic inhibition of cocaine seeking in rats Stefanik, Michael T Moussawi, Khaled Kupchik, Yonatan M Smith, Kyle C Miller, Rachel L Huff, Mary L Deisseroth, Karl Kalivas, Peter W LaLumiere, Ryan T Addict Biol Preclinical Studies Inhibitory optogenetics was used to examine the roles of the prelimbic cortex (PL), the nucleus accumbens core (NAcore) and the PL projections to the NAcore in the reinstatement of cocaine seeking. Rats were microinjected into the PL or NAcore with an adeno-associated virus containing halorhodopsin or archaerhodopsin. After 12 days of cocaine self-administration, followed by extinction training, animals underwent reinstatement testing along with the presence/absence of optically induced inhibition via laser light. Bilateral optical inhibition of the PL, NAcore or the PL fibers in the NAcore inhibited the reinstatement of cocaine seeking. Blackwell Publishing Ltd 2013-01 2012-07-24 /pmc/articles/PMC3578202/ /pubmed/22823160 http://dx.doi.org/10.1111/j.1369-1600.2012.00479.x Text en © 2013 Society for the Study of Addiction http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Preclinical Studies
Stefanik, Michael T
Moussawi, Khaled
Kupchik, Yonatan M
Smith, Kyle C
Miller, Rachel L
Huff, Mary L
Deisseroth, Karl
Kalivas, Peter W
LaLumiere, Ryan T
Optogenetic inhibition of cocaine seeking in rats
title Optogenetic inhibition of cocaine seeking in rats
title_full Optogenetic inhibition of cocaine seeking in rats
title_fullStr Optogenetic inhibition of cocaine seeking in rats
title_full_unstemmed Optogenetic inhibition of cocaine seeking in rats
title_short Optogenetic inhibition of cocaine seeking in rats
title_sort optogenetic inhibition of cocaine seeking in rats
topic Preclinical Studies
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3578202/
https://www.ncbi.nlm.nih.gov/pubmed/22823160
http://dx.doi.org/10.1111/j.1369-1600.2012.00479.x
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