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MAP65 Coordinate Microtubule Growth during Bundle Formation

Microtubules (MTs) are highly dynamical structures that play a crucial role in cell physiology. In cooperation with microtubule-associated proteins (MAPs), MTs form bundles endowing cells with specific mechanisms to control their shape or generate forces. Whether the dynamics of MTs is affected by t...

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Autores principales: Stoppin-Mellet, Virginie, Fache, Vincent, Portran, Didier, Martiel, Jean-Louis, Vantard, Marylin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3578873/
https://www.ncbi.nlm.nih.gov/pubmed/23437247
http://dx.doi.org/10.1371/journal.pone.0056808
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author Stoppin-Mellet, Virginie
Fache, Vincent
Portran, Didier
Martiel, Jean-Louis
Vantard, Marylin
author_facet Stoppin-Mellet, Virginie
Fache, Vincent
Portran, Didier
Martiel, Jean-Louis
Vantard, Marylin
author_sort Stoppin-Mellet, Virginie
collection PubMed
description Microtubules (MTs) are highly dynamical structures that play a crucial role in cell physiology. In cooperation with microtubule-associated proteins (MAPs), MTs form bundles endowing cells with specific mechanisms to control their shape or generate forces. Whether the dynamics of MTs is affected by the lateral connections that MAPs make between MTs during bundle formation is still under debate. Using in vitro reconstitution of MT bundling, we analyzed the dynamics of MT bundles generated by two plant MAP65 (MAP65-1/4), MAP65-1 being the plant ortholog of vertebrate PRC1 and yeast Ase1. MAP65-1/4 limit the amplitude of MT bundle depolymerization and increase the elongation phases. The subsequent sustained elongation of bundles is governed by the coordination of MT growth, so that MT ends come in close vicinity. We develop a model based on the assumption that both MAP65-1/4 block MT depolymerization. Model simulations reveal that rescue frequencies are higher between parallel than between anti-parallel MTs. In consequence the polarity of bundled MTs by MAP65 controls the amplitude of bundle’s growth. Our results illustrate how MAP-induced MT-bundling, which is finely tuned by MT polarity, robustly coordinates MT elongation within bundles.
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spelling pubmed-35788732013-02-22 MAP65 Coordinate Microtubule Growth during Bundle Formation Stoppin-Mellet, Virginie Fache, Vincent Portran, Didier Martiel, Jean-Louis Vantard, Marylin PLoS One Research Article Microtubules (MTs) are highly dynamical structures that play a crucial role in cell physiology. In cooperation with microtubule-associated proteins (MAPs), MTs form bundles endowing cells with specific mechanisms to control their shape or generate forces. Whether the dynamics of MTs is affected by the lateral connections that MAPs make between MTs during bundle formation is still under debate. Using in vitro reconstitution of MT bundling, we analyzed the dynamics of MT bundles generated by two plant MAP65 (MAP65-1/4), MAP65-1 being the plant ortholog of vertebrate PRC1 and yeast Ase1. MAP65-1/4 limit the amplitude of MT bundle depolymerization and increase the elongation phases. The subsequent sustained elongation of bundles is governed by the coordination of MT growth, so that MT ends come in close vicinity. We develop a model based on the assumption that both MAP65-1/4 block MT depolymerization. Model simulations reveal that rescue frequencies are higher between parallel than between anti-parallel MTs. In consequence the polarity of bundled MTs by MAP65 controls the amplitude of bundle’s growth. Our results illustrate how MAP-induced MT-bundling, which is finely tuned by MT polarity, robustly coordinates MT elongation within bundles. Public Library of Science 2013-02-21 /pmc/articles/PMC3578873/ /pubmed/23437247 http://dx.doi.org/10.1371/journal.pone.0056808 Text en © 2013 Stoppin-Mellet et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Stoppin-Mellet, Virginie
Fache, Vincent
Portran, Didier
Martiel, Jean-Louis
Vantard, Marylin
MAP65 Coordinate Microtubule Growth during Bundle Formation
title MAP65 Coordinate Microtubule Growth during Bundle Formation
title_full MAP65 Coordinate Microtubule Growth during Bundle Formation
title_fullStr MAP65 Coordinate Microtubule Growth during Bundle Formation
title_full_unstemmed MAP65 Coordinate Microtubule Growth during Bundle Formation
title_short MAP65 Coordinate Microtubule Growth during Bundle Formation
title_sort map65 coordinate microtubule growth during bundle formation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3578873/
https://www.ncbi.nlm.nih.gov/pubmed/23437247
http://dx.doi.org/10.1371/journal.pone.0056808
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