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Volatile constituents of Pinus roxburghii from Nepal

BACKGROUND: Pinus roxburghii Sarg. Is one of 3 species of pine found in Nepal, the oil of which is traditionally used to treat cuts, wounds, boils, and blisters. OBJECTIVE: To obtain, analyze, and examine the anti-microbial and cytotoxic activities of the essential oils of P. roxburghii. MATERIALS A...

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Autores principales: Satyal, Prabodh, Paudel, Prajwal, Raut, Josna, Deo, Akash, Dosoky, Noura S., Setzer, William N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3579019/
https://www.ncbi.nlm.nih.gov/pubmed/23598924
http://dx.doi.org/10.4103/0974-8490.105650
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author Satyal, Prabodh
Paudel, Prajwal
Raut, Josna
Deo, Akash
Dosoky, Noura S.
Setzer, William N.
author_facet Satyal, Prabodh
Paudel, Prajwal
Raut, Josna
Deo, Akash
Dosoky, Noura S.
Setzer, William N.
author_sort Satyal, Prabodh
collection PubMed
description BACKGROUND: Pinus roxburghii Sarg. Is one of 3 species of pine found in Nepal, the oil of which is traditionally used to treat cuts, wounds, boils, and blisters. OBJECTIVE: To obtain, analyze, and examine the anti-microbial and cytotoxic activities of the essential oils of P. roxburghii. MATERIALS AND METHODS: Three plant parts (cone, needle, and bark) of Pinus roxburghii were collected in Biratnagar, Nepal. The essential oils were obtained by hydrodistillation, and the chemical compositions were determined by GC-MS. The needle and cone essential oils were screened for anti-microbial activity against Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Aspergillus niger; brine shrimp (Artemia salina) lethality; and in-vitro cytotoxicity against MCF-7 cells. RESULTS: GC-MS analysis for the cone oil revealed 81 compounds with 78 components being identified (95.5% of the oil) while 98.3% of needle oil was identified to contain 68 components and 98.6% of the bark oil (38 components) was identified. The 3 essential oils were dominated by sesquiterpenes, particularly (E)-caryophyllene (26.8%-34.5%) and α-humulene (5.0%-7.3%) as well as monoterpene alcohols terpinen-4-ol (4.1%-30.1%) and α-terpineol(2.8%-5.0%). The monoterpene δ-3-carene was present only in needle and cone essential oils (2.3% and 6.8%, respectively). Bio-activity assays of the cone essential oil of P. roxburghii showed remarkable cytotoxic activity (100% killing of MCF-7 cells at 100 μg/mL) along with notable brine shrimp lethality (LC(50) =11.8 μg/mL). The cone essential oil did not show anti-bacterial activity, but it did exhibit anti-fungal activity against Aspergillus niger (MIC=39 μg/mL). CONCLUSION: The bioactivity of P. roxburghii essential oil is consistent with its traditional medicinal use.
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spelling pubmed-35790192013-02-28 Volatile constituents of Pinus roxburghii from Nepal Satyal, Prabodh Paudel, Prajwal Raut, Josna Deo, Akash Dosoky, Noura S. Setzer, William N. Pharmacognosy Res Original Article BACKGROUND: Pinus roxburghii Sarg. Is one of 3 species of pine found in Nepal, the oil of which is traditionally used to treat cuts, wounds, boils, and blisters. OBJECTIVE: To obtain, analyze, and examine the anti-microbial and cytotoxic activities of the essential oils of P. roxburghii. MATERIALS AND METHODS: Three plant parts (cone, needle, and bark) of Pinus roxburghii were collected in Biratnagar, Nepal. The essential oils were obtained by hydrodistillation, and the chemical compositions were determined by GC-MS. The needle and cone essential oils were screened for anti-microbial activity against Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Aspergillus niger; brine shrimp (Artemia salina) lethality; and in-vitro cytotoxicity against MCF-7 cells. RESULTS: GC-MS analysis for the cone oil revealed 81 compounds with 78 components being identified (95.5% of the oil) while 98.3% of needle oil was identified to contain 68 components and 98.6% of the bark oil (38 components) was identified. The 3 essential oils were dominated by sesquiterpenes, particularly (E)-caryophyllene (26.8%-34.5%) and α-humulene (5.0%-7.3%) as well as monoterpene alcohols terpinen-4-ol (4.1%-30.1%) and α-terpineol(2.8%-5.0%). The monoterpene δ-3-carene was present only in needle and cone essential oils (2.3% and 6.8%, respectively). Bio-activity assays of the cone essential oil of P. roxburghii showed remarkable cytotoxic activity (100% killing of MCF-7 cells at 100 μg/mL) along with notable brine shrimp lethality (LC(50) =11.8 μg/mL). The cone essential oil did not show anti-bacterial activity, but it did exhibit anti-fungal activity against Aspergillus niger (MIC=39 μg/mL). CONCLUSION: The bioactivity of P. roxburghii essential oil is consistent with its traditional medicinal use. Medknow Publications & Media Pvt Ltd 2013 /pmc/articles/PMC3579019/ /pubmed/23598924 http://dx.doi.org/10.4103/0974-8490.105650 Text en Copyright: © Pharmacognosy Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Satyal, Prabodh
Paudel, Prajwal
Raut, Josna
Deo, Akash
Dosoky, Noura S.
Setzer, William N.
Volatile constituents of Pinus roxburghii from Nepal
title Volatile constituents of Pinus roxburghii from Nepal
title_full Volatile constituents of Pinus roxburghii from Nepal
title_fullStr Volatile constituents of Pinus roxburghii from Nepal
title_full_unstemmed Volatile constituents of Pinus roxburghii from Nepal
title_short Volatile constituents of Pinus roxburghii from Nepal
title_sort volatile constituents of pinus roxburghii from nepal
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3579019/
https://www.ncbi.nlm.nih.gov/pubmed/23598924
http://dx.doi.org/10.4103/0974-8490.105650
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