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Small molecules inhibit growth, viability and ergosterol biosynthesis in Candida albicans
The aim of this work was to evaluate the anti-Candida efficacy of twenty five molecules of plant origin. Based on their MICs, effective molecules were categorized into four categories. Susceptibility testing of test compounds was carried out by standard methodology (M27-A2) as per CLSI guidelines. M...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing AG
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3579468/ https://www.ncbi.nlm.nih.gov/pubmed/23449869 http://dx.doi.org/10.1186/2193-1801-2-26 |
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author | Rajput, Sandeep B Karuppayil, S Mohan |
author_facet | Rajput, Sandeep B Karuppayil, S Mohan |
author_sort | Rajput, Sandeep B |
collection | PubMed |
description | The aim of this work was to evaluate the anti-Candida efficacy of twenty five molecules of plant origin. Based on their MICs, effective molecules were categorized into four categories. Susceptibility testing of test compounds was carried out by standard methodology (M27-A2) as per CLSI guidelines. Minimum Fungicidal Concentration (MFC) was determined as the lowest concentration of drug killing 99.9% cells. Effect on sterol profile was evaluated by sterol quantitation method. Among the screened molecules, cinnamaldehyde, piperidine, citral, furfuraldehyde and indole were potent inhibitors of growth and viability. Exposure of Candida cells to cinnamaldehyde, piperidine, citral, furfuraldehyde, indole, α- and β- pinene at MIC’s, altered ergosterol profile. Our results indicate that the molecules altering sterol profile may exert their antifungal effect through inhibition of ergosterol biosynthesis and could be good candidates for fungal specific drug development. |
format | Online Article Text |
id | pubmed-3579468 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Springer International Publishing AG |
record_format | MEDLINE/PubMed |
spelling | pubmed-35794682013-02-26 Small molecules inhibit growth, viability and ergosterol biosynthesis in Candida albicans Rajput, Sandeep B Karuppayil, S Mohan Springerplus Research The aim of this work was to evaluate the anti-Candida efficacy of twenty five molecules of plant origin. Based on their MICs, effective molecules were categorized into four categories. Susceptibility testing of test compounds was carried out by standard methodology (M27-A2) as per CLSI guidelines. Minimum Fungicidal Concentration (MFC) was determined as the lowest concentration of drug killing 99.9% cells. Effect on sterol profile was evaluated by sterol quantitation method. Among the screened molecules, cinnamaldehyde, piperidine, citral, furfuraldehyde and indole were potent inhibitors of growth and viability. Exposure of Candida cells to cinnamaldehyde, piperidine, citral, furfuraldehyde, indole, α- and β- pinene at MIC’s, altered ergosterol profile. Our results indicate that the molecules altering sterol profile may exert their antifungal effect through inhibition of ergosterol biosynthesis and could be good candidates for fungal specific drug development. Springer International Publishing AG 2013-01-29 /pmc/articles/PMC3579468/ /pubmed/23449869 http://dx.doi.org/10.1186/2193-1801-2-26 Text en © Rajput and Karuppayil; licensee Springer. 2013 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Rajput, Sandeep B Karuppayil, S Mohan Small molecules inhibit growth, viability and ergosterol biosynthesis in Candida albicans |
title | Small molecules inhibit growth, viability and ergosterol biosynthesis in Candida albicans |
title_full | Small molecules inhibit growth, viability and ergosterol biosynthesis in Candida albicans |
title_fullStr | Small molecules inhibit growth, viability and ergosterol biosynthesis in Candida albicans |
title_full_unstemmed | Small molecules inhibit growth, viability and ergosterol biosynthesis in Candida albicans |
title_short | Small molecules inhibit growth, viability and ergosterol biosynthesis in Candida albicans |
title_sort | small molecules inhibit growth, viability and ergosterol biosynthesis in candida albicans |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3579468/ https://www.ncbi.nlm.nih.gov/pubmed/23449869 http://dx.doi.org/10.1186/2193-1801-2-26 |
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