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In Vivo Imaging of Transplanted Islets Labeled with a Novel Cationic Nanoparticle

To monitor pancreatic islet transplantation efficiency, reliable noninvasive imaging methods, such as magnetic resonance imaging (MRI) are needed. Although an efficient uptake of MRI contrast agent is required for islet cell labeling, commercially-available magnetic nanoparticles are not efficiently...

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Autores principales: Oishi, Koichi, Miyamoto, Yoshitaka, Saito, Hiroaki, Murase, Katsutoshi, Ono, Kenji, Sawada, Makoto, Watanabe, Masami, Noguchi, Yasufumi, Fujiwara, Toshiyoshi, Hayashi, Shuji, Noguchi, Hirofumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3579774/
https://www.ncbi.nlm.nih.gov/pubmed/23451139
http://dx.doi.org/10.1371/journal.pone.0057046
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author Oishi, Koichi
Miyamoto, Yoshitaka
Saito, Hiroaki
Murase, Katsutoshi
Ono, Kenji
Sawada, Makoto
Watanabe, Masami
Noguchi, Yasufumi
Fujiwara, Toshiyoshi
Hayashi, Shuji
Noguchi, Hirofumi
author_facet Oishi, Koichi
Miyamoto, Yoshitaka
Saito, Hiroaki
Murase, Katsutoshi
Ono, Kenji
Sawada, Makoto
Watanabe, Masami
Noguchi, Yasufumi
Fujiwara, Toshiyoshi
Hayashi, Shuji
Noguchi, Hirofumi
author_sort Oishi, Koichi
collection PubMed
description To monitor pancreatic islet transplantation efficiency, reliable noninvasive imaging methods, such as magnetic resonance imaging (MRI) are needed. Although an efficient uptake of MRI contrast agent is required for islet cell labeling, commercially-available magnetic nanoparticles are not efficiently transduced into cells. We herein report the in vivo detection of transplanted islets labeled with a novel cationic nanoparticle that allowed for noninvasive monitoring of islet grafts in diabetic mice in real time. The positively-charged nanoparticles were transduced into a β-cell line, MIN6 cells, and into isolated islets for 1 hr. MRI showed a marked decrease in the signal intensity on T1- and T2-weighted images at the implantation site of the labeled MIN 6 cells or islets in the left kidneys of mice. These data suggest that the novel positively-charged nanoparticle could be useful to detect and monitor islet engraftment, which would greatly aid in the clinical management of islet transplant patients.
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spelling pubmed-35797742013-02-28 In Vivo Imaging of Transplanted Islets Labeled with a Novel Cationic Nanoparticle Oishi, Koichi Miyamoto, Yoshitaka Saito, Hiroaki Murase, Katsutoshi Ono, Kenji Sawada, Makoto Watanabe, Masami Noguchi, Yasufumi Fujiwara, Toshiyoshi Hayashi, Shuji Noguchi, Hirofumi PLoS One Research Article To monitor pancreatic islet transplantation efficiency, reliable noninvasive imaging methods, such as magnetic resonance imaging (MRI) are needed. Although an efficient uptake of MRI contrast agent is required for islet cell labeling, commercially-available magnetic nanoparticles are not efficiently transduced into cells. We herein report the in vivo detection of transplanted islets labeled with a novel cationic nanoparticle that allowed for noninvasive monitoring of islet grafts in diabetic mice in real time. The positively-charged nanoparticles were transduced into a β-cell line, MIN6 cells, and into isolated islets for 1 hr. MRI showed a marked decrease in the signal intensity on T1- and T2-weighted images at the implantation site of the labeled MIN 6 cells or islets in the left kidneys of mice. These data suggest that the novel positively-charged nanoparticle could be useful to detect and monitor islet engraftment, which would greatly aid in the clinical management of islet transplant patients. Public Library of Science 2013-02-22 /pmc/articles/PMC3579774/ /pubmed/23451139 http://dx.doi.org/10.1371/journal.pone.0057046 Text en © 2013 Oishi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Oishi, Koichi
Miyamoto, Yoshitaka
Saito, Hiroaki
Murase, Katsutoshi
Ono, Kenji
Sawada, Makoto
Watanabe, Masami
Noguchi, Yasufumi
Fujiwara, Toshiyoshi
Hayashi, Shuji
Noguchi, Hirofumi
In Vivo Imaging of Transplanted Islets Labeled with a Novel Cationic Nanoparticle
title In Vivo Imaging of Transplanted Islets Labeled with a Novel Cationic Nanoparticle
title_full In Vivo Imaging of Transplanted Islets Labeled with a Novel Cationic Nanoparticle
title_fullStr In Vivo Imaging of Transplanted Islets Labeled with a Novel Cationic Nanoparticle
title_full_unstemmed In Vivo Imaging of Transplanted Islets Labeled with a Novel Cationic Nanoparticle
title_short In Vivo Imaging of Transplanted Islets Labeled with a Novel Cationic Nanoparticle
title_sort in vivo imaging of transplanted islets labeled with a novel cationic nanoparticle
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3579774/
https://www.ncbi.nlm.nih.gov/pubmed/23451139
http://dx.doi.org/10.1371/journal.pone.0057046
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