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In Vivo Imaging of Transplanted Islets Labeled with a Novel Cationic Nanoparticle
To monitor pancreatic islet transplantation efficiency, reliable noninvasive imaging methods, such as magnetic resonance imaging (MRI) are needed. Although an efficient uptake of MRI contrast agent is required for islet cell labeling, commercially-available magnetic nanoparticles are not efficiently...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3579774/ https://www.ncbi.nlm.nih.gov/pubmed/23451139 http://dx.doi.org/10.1371/journal.pone.0057046 |
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author | Oishi, Koichi Miyamoto, Yoshitaka Saito, Hiroaki Murase, Katsutoshi Ono, Kenji Sawada, Makoto Watanabe, Masami Noguchi, Yasufumi Fujiwara, Toshiyoshi Hayashi, Shuji Noguchi, Hirofumi |
author_facet | Oishi, Koichi Miyamoto, Yoshitaka Saito, Hiroaki Murase, Katsutoshi Ono, Kenji Sawada, Makoto Watanabe, Masami Noguchi, Yasufumi Fujiwara, Toshiyoshi Hayashi, Shuji Noguchi, Hirofumi |
author_sort | Oishi, Koichi |
collection | PubMed |
description | To monitor pancreatic islet transplantation efficiency, reliable noninvasive imaging methods, such as magnetic resonance imaging (MRI) are needed. Although an efficient uptake of MRI contrast agent is required for islet cell labeling, commercially-available magnetic nanoparticles are not efficiently transduced into cells. We herein report the in vivo detection of transplanted islets labeled with a novel cationic nanoparticle that allowed for noninvasive monitoring of islet grafts in diabetic mice in real time. The positively-charged nanoparticles were transduced into a β-cell line, MIN6 cells, and into isolated islets for 1 hr. MRI showed a marked decrease in the signal intensity on T1- and T2-weighted images at the implantation site of the labeled MIN 6 cells or islets in the left kidneys of mice. These data suggest that the novel positively-charged nanoparticle could be useful to detect and monitor islet engraftment, which would greatly aid in the clinical management of islet transplant patients. |
format | Online Article Text |
id | pubmed-3579774 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35797742013-02-28 In Vivo Imaging of Transplanted Islets Labeled with a Novel Cationic Nanoparticle Oishi, Koichi Miyamoto, Yoshitaka Saito, Hiroaki Murase, Katsutoshi Ono, Kenji Sawada, Makoto Watanabe, Masami Noguchi, Yasufumi Fujiwara, Toshiyoshi Hayashi, Shuji Noguchi, Hirofumi PLoS One Research Article To monitor pancreatic islet transplantation efficiency, reliable noninvasive imaging methods, such as magnetic resonance imaging (MRI) are needed. Although an efficient uptake of MRI contrast agent is required for islet cell labeling, commercially-available magnetic nanoparticles are not efficiently transduced into cells. We herein report the in vivo detection of transplanted islets labeled with a novel cationic nanoparticle that allowed for noninvasive monitoring of islet grafts in diabetic mice in real time. The positively-charged nanoparticles were transduced into a β-cell line, MIN6 cells, and into isolated islets for 1 hr. MRI showed a marked decrease in the signal intensity on T1- and T2-weighted images at the implantation site of the labeled MIN 6 cells or islets in the left kidneys of mice. These data suggest that the novel positively-charged nanoparticle could be useful to detect and monitor islet engraftment, which would greatly aid in the clinical management of islet transplant patients. Public Library of Science 2013-02-22 /pmc/articles/PMC3579774/ /pubmed/23451139 http://dx.doi.org/10.1371/journal.pone.0057046 Text en © 2013 Oishi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Oishi, Koichi Miyamoto, Yoshitaka Saito, Hiroaki Murase, Katsutoshi Ono, Kenji Sawada, Makoto Watanabe, Masami Noguchi, Yasufumi Fujiwara, Toshiyoshi Hayashi, Shuji Noguchi, Hirofumi In Vivo Imaging of Transplanted Islets Labeled with a Novel Cationic Nanoparticle |
title | In Vivo Imaging of Transplanted Islets Labeled with a Novel Cationic Nanoparticle |
title_full | In Vivo Imaging of Transplanted Islets Labeled with a Novel Cationic Nanoparticle |
title_fullStr | In Vivo Imaging of Transplanted Islets Labeled with a Novel Cationic Nanoparticle |
title_full_unstemmed | In Vivo Imaging of Transplanted Islets Labeled with a Novel Cationic Nanoparticle |
title_short | In Vivo Imaging of Transplanted Islets Labeled with a Novel Cationic Nanoparticle |
title_sort | in vivo imaging of transplanted islets labeled with a novel cationic nanoparticle |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3579774/ https://www.ncbi.nlm.nih.gov/pubmed/23451139 http://dx.doi.org/10.1371/journal.pone.0057046 |
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