Cargando…

Prefabrication of vascularized bone graft using an interconnected porous calcium hydroxyapatite ceramic in presence of vascular endothelial growth factor and bone marrow mesenchymal stem cells: Experimental study in rats

OBJECTIVES: The purpose of this experimental pilot study was to create a prefabricated vascularized bone graft using interconnected porous calcium hydroxyapatite ceramic (PCHC) block by combining vascular bundle implantation, rat bone marrow mesenchymal stem cells and administration of vascular endo...

Descripción completa

Detalles Bibliográficos
Autores principales: Sever, Celalettin, Uygur, Fatih, Kose, Gamze Torun, Urhan, Muammer, Haholu, Abdullah, Kulahci, Yalcin, Sinan, Oksuz, Cihan, Sahin, Omer, Ozcan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3580341/
https://www.ncbi.nlm.nih.gov/pubmed/23450373
http://dx.doi.org/10.4103/0970-0358.105939
_version_ 1782260225907097600
author Sever, Celalettin
Uygur, Fatih
Kose, Gamze Torun
Urhan, Muammer
Haholu, Abdullah
Kulahci, Yalcin
Sinan, Oksuz
Cihan, Sahin
Omer, Ozcan
author_facet Sever, Celalettin
Uygur, Fatih
Kose, Gamze Torun
Urhan, Muammer
Haholu, Abdullah
Kulahci, Yalcin
Sinan, Oksuz
Cihan, Sahin
Omer, Ozcan
author_sort Sever, Celalettin
collection PubMed
description OBJECTIVES: The purpose of this experimental pilot study was to create a prefabricated vascularized bone graft using interconnected porous calcium hydroxyapatite ceramic (PCHC) block by combining vascular bundle implantation, rat bone marrow mesenchymal stem cells and administration of vascular endothelial growth factor (VEGF) in a rat model. MATERIALS AND METHODS: Sixty male Sprague-Dawley rats were used. Experimental animals were divided into six groups, each of which comprised 10 rats. The PCHC blocks were implanted in the medial thigh region in groups I, III, and V without vascular bundle implantation. The PCHC blocks were vascularized by the superficial inferior epigastric artery and vein in groups II, IV and VI. These vessels were passed through the hole of the PCHC blocks. Mesenchymal stem cells were administered into the PCHC in groups III, IV, V and VI. In addition, both mesenchymal stem cells and VEGF were administered in group V and VI. The presence and density of any new bone formation and neovascularization from the vascular bundle was evaluated by X-ray, microangiography, scintigraphy, biochemical analysis and histomorphometry. RESULTS: The newly formed vessels and bone formations were significantly greater in group VI, in which both mesenchymal stem cells and VEGF were applied. CONCLUSION: This preliminary study suggests that: Both mesenchymal stem cells and VEGF provide vascularized bone prefabrication by enhancing neovascularization and osteogenesis in a shorter time compared to only VEGF application.
format Online
Article
Text
id pubmed-3580341
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Medknow Publications & Media Pvt Ltd
record_format MEDLINE/PubMed
spelling pubmed-35803412013-02-28 Prefabrication of vascularized bone graft using an interconnected porous calcium hydroxyapatite ceramic in presence of vascular endothelial growth factor and bone marrow mesenchymal stem cells: Experimental study in rats Sever, Celalettin Uygur, Fatih Kose, Gamze Torun Urhan, Muammer Haholu, Abdullah Kulahci, Yalcin Sinan, Oksuz Cihan, Sahin Omer, Ozcan Indian J Plast Surg Original Article OBJECTIVES: The purpose of this experimental pilot study was to create a prefabricated vascularized bone graft using interconnected porous calcium hydroxyapatite ceramic (PCHC) block by combining vascular bundle implantation, rat bone marrow mesenchymal stem cells and administration of vascular endothelial growth factor (VEGF) in a rat model. MATERIALS AND METHODS: Sixty male Sprague-Dawley rats were used. Experimental animals were divided into six groups, each of which comprised 10 rats. The PCHC blocks were implanted in the medial thigh region in groups I, III, and V without vascular bundle implantation. The PCHC blocks were vascularized by the superficial inferior epigastric artery and vein in groups II, IV and VI. These vessels were passed through the hole of the PCHC blocks. Mesenchymal stem cells were administered into the PCHC in groups III, IV, V and VI. In addition, both mesenchymal stem cells and VEGF were administered in group V and VI. The presence and density of any new bone formation and neovascularization from the vascular bundle was evaluated by X-ray, microangiography, scintigraphy, biochemical analysis and histomorphometry. RESULTS: The newly formed vessels and bone formations were significantly greater in group VI, in which both mesenchymal stem cells and VEGF were applied. CONCLUSION: This preliminary study suggests that: Both mesenchymal stem cells and VEGF provide vascularized bone prefabrication by enhancing neovascularization and osteogenesis in a shorter time compared to only VEGF application. Medknow Publications & Media Pvt Ltd 2012 /pmc/articles/PMC3580341/ /pubmed/23450373 http://dx.doi.org/10.4103/0970-0358.105939 Text en Copyright: © Indian Journal of Plastic Surgery http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Sever, Celalettin
Uygur, Fatih
Kose, Gamze Torun
Urhan, Muammer
Haholu, Abdullah
Kulahci, Yalcin
Sinan, Oksuz
Cihan, Sahin
Omer, Ozcan
Prefabrication of vascularized bone graft using an interconnected porous calcium hydroxyapatite ceramic in presence of vascular endothelial growth factor and bone marrow mesenchymal stem cells: Experimental study in rats
title Prefabrication of vascularized bone graft using an interconnected porous calcium hydroxyapatite ceramic in presence of vascular endothelial growth factor and bone marrow mesenchymal stem cells: Experimental study in rats
title_full Prefabrication of vascularized bone graft using an interconnected porous calcium hydroxyapatite ceramic in presence of vascular endothelial growth factor and bone marrow mesenchymal stem cells: Experimental study in rats
title_fullStr Prefabrication of vascularized bone graft using an interconnected porous calcium hydroxyapatite ceramic in presence of vascular endothelial growth factor and bone marrow mesenchymal stem cells: Experimental study in rats
title_full_unstemmed Prefabrication of vascularized bone graft using an interconnected porous calcium hydroxyapatite ceramic in presence of vascular endothelial growth factor and bone marrow mesenchymal stem cells: Experimental study in rats
title_short Prefabrication of vascularized bone graft using an interconnected porous calcium hydroxyapatite ceramic in presence of vascular endothelial growth factor and bone marrow mesenchymal stem cells: Experimental study in rats
title_sort prefabrication of vascularized bone graft using an interconnected porous calcium hydroxyapatite ceramic in presence of vascular endothelial growth factor and bone marrow mesenchymal stem cells: experimental study in rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3580341/
https://www.ncbi.nlm.nih.gov/pubmed/23450373
http://dx.doi.org/10.4103/0970-0358.105939
work_keys_str_mv AT severcelalettin prefabricationofvascularizedbonegraftusinganinterconnectedporouscalciumhydroxyapatiteceramicinpresenceofvascularendothelialgrowthfactorandbonemarrowmesenchymalstemcellsexperimentalstudyinrats
AT uygurfatih prefabricationofvascularizedbonegraftusinganinterconnectedporouscalciumhydroxyapatiteceramicinpresenceofvascularendothelialgrowthfactorandbonemarrowmesenchymalstemcellsexperimentalstudyinrats
AT kosegamzetorun prefabricationofvascularizedbonegraftusinganinterconnectedporouscalciumhydroxyapatiteceramicinpresenceofvascularendothelialgrowthfactorandbonemarrowmesenchymalstemcellsexperimentalstudyinrats
AT urhanmuammer prefabricationofvascularizedbonegraftusinganinterconnectedporouscalciumhydroxyapatiteceramicinpresenceofvascularendothelialgrowthfactorandbonemarrowmesenchymalstemcellsexperimentalstudyinrats
AT haholuabdullah prefabricationofvascularizedbonegraftusinganinterconnectedporouscalciumhydroxyapatiteceramicinpresenceofvascularendothelialgrowthfactorandbonemarrowmesenchymalstemcellsexperimentalstudyinrats
AT kulahciyalcin prefabricationofvascularizedbonegraftusinganinterconnectedporouscalciumhydroxyapatiteceramicinpresenceofvascularendothelialgrowthfactorandbonemarrowmesenchymalstemcellsexperimentalstudyinrats
AT sinanoksuz prefabricationofvascularizedbonegraftusinganinterconnectedporouscalciumhydroxyapatiteceramicinpresenceofvascularendothelialgrowthfactorandbonemarrowmesenchymalstemcellsexperimentalstudyinrats
AT cihansahin prefabricationofvascularizedbonegraftusinganinterconnectedporouscalciumhydroxyapatiteceramicinpresenceofvascularendothelialgrowthfactorandbonemarrowmesenchymalstemcellsexperimentalstudyinrats
AT omerozcan prefabricationofvascularizedbonegraftusinganinterconnectedporouscalciumhydroxyapatiteceramicinpresenceofvascularendothelialgrowthfactorandbonemarrowmesenchymalstemcellsexperimentalstudyinrats