A phenotypic screening platform to identify small molecule modulators of Chlamydomonas reinhardtii growth, motility and photosynthesis

Chemical biology, the interfacial discipline of using small molecules as probes to investigate biology, is a powerful approach of developing specific, rapidly acting tools that can be applied across organisms. The single-celled alga Chlamydomonas reinhardtii is an excellent model system because of i...

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Autores principales: Alfred, Simon E, Surendra, Anuradha, Le, Chris, Lin, Ken, Mok, Alexander, Wallace, Iain M, Proctor, Michael, Urbanus, Malene L, Giaever, Guri, Nislow, Corey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3580497/
https://www.ncbi.nlm.nih.gov/pubmed/23158586
http://dx.doi.org/10.1186/gb-2012-13-11-r105
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author Alfred, Simon E
Surendra, Anuradha
Le, Chris
Lin, Ken
Mok, Alexander
Wallace, Iain M
Proctor, Michael
Urbanus, Malene L
Giaever, Guri
Nislow, Corey
author_facet Alfred, Simon E
Surendra, Anuradha
Le, Chris
Lin, Ken
Mok, Alexander
Wallace, Iain M
Proctor, Michael
Urbanus, Malene L
Giaever, Guri
Nislow, Corey
author_sort Alfred, Simon E
collection PubMed
description Chemical biology, the interfacial discipline of using small molecules as probes to investigate biology, is a powerful approach of developing specific, rapidly acting tools that can be applied across organisms. The single-celled alga Chlamydomonas reinhardtii is an excellent model system because of its photosynthetic ability, cilia-related motility and simple genetics. We report the results of an automated fitness screen of 5,445 small molecules and subsequent assays on motility/phototaxis and photosynthesis. Cheminformatic analysis revealed active core structures and was used to construct a naïve Bayes model that successfully predicts algal bioactive compounds.
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spelling pubmed-35804972013-02-26 A phenotypic screening platform to identify small molecule modulators of Chlamydomonas reinhardtii growth, motility and photosynthesis Alfred, Simon E Surendra, Anuradha Le, Chris Lin, Ken Mok, Alexander Wallace, Iain M Proctor, Michael Urbanus, Malene L Giaever, Guri Nislow, Corey Genome Biol Method Chemical biology, the interfacial discipline of using small molecules as probes to investigate biology, is a powerful approach of developing specific, rapidly acting tools that can be applied across organisms. The single-celled alga Chlamydomonas reinhardtii is an excellent model system because of its photosynthetic ability, cilia-related motility and simple genetics. We report the results of an automated fitness screen of 5,445 small molecules and subsequent assays on motility/phototaxis and photosynthesis. Cheminformatic analysis revealed active core structures and was used to construct a naïve Bayes model that successfully predicts algal bioactive compounds. BioMed Central 2012 2012-11-18 /pmc/articles/PMC3580497/ /pubmed/23158586 http://dx.doi.org/10.1186/gb-2012-13-11-r105 Text en Copyright ©2012 Alfred et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
Alfred, Simon E
Surendra, Anuradha
Le, Chris
Lin, Ken
Mok, Alexander
Wallace, Iain M
Proctor, Michael
Urbanus, Malene L
Giaever, Guri
Nislow, Corey
A phenotypic screening platform to identify small molecule modulators of Chlamydomonas reinhardtii growth, motility and photosynthesis
title A phenotypic screening platform to identify small molecule modulators of Chlamydomonas reinhardtii growth, motility and photosynthesis
title_full A phenotypic screening platform to identify small molecule modulators of Chlamydomonas reinhardtii growth, motility and photosynthesis
title_fullStr A phenotypic screening platform to identify small molecule modulators of Chlamydomonas reinhardtii growth, motility and photosynthesis
title_full_unstemmed A phenotypic screening platform to identify small molecule modulators of Chlamydomonas reinhardtii growth, motility and photosynthesis
title_short A phenotypic screening platform to identify small molecule modulators of Chlamydomonas reinhardtii growth, motility and photosynthesis
title_sort phenotypic screening platform to identify small molecule modulators of chlamydomonas reinhardtii growth, motility and photosynthesis
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3580497/
https://www.ncbi.nlm.nih.gov/pubmed/23158586
http://dx.doi.org/10.1186/gb-2012-13-11-r105
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