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Quantification of individual proteins in silicone hydrogel contact lens deposits

PURPOSE: The aim of this study was to quantify specific proteins deposited on daily wear silicone hydrogel lenses used in combination with multipurpose disinfecting solutions (MPDSs) by applying multiple-reaction-monitoring mass spectrometry (MRM-MS). METHODS: Balafilcon A or senofilcon A contact le...

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Autores principales: Omali, Negar Babaei, Zhao, Zhenjun, Zhu, Hua, Tilia, Daniel, Willcox, Mark D.P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Vision 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3580989/
https://www.ncbi.nlm.nih.gov/pubmed/23441110
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author Omali, Negar Babaei
Zhao, Zhenjun
Zhu, Hua
Tilia, Daniel
Willcox, Mark D.P.
author_facet Omali, Negar Babaei
Zhao, Zhenjun
Zhu, Hua
Tilia, Daniel
Willcox, Mark D.P.
author_sort Omali, Negar Babaei
collection PubMed
description PURPOSE: The aim of this study was to quantify specific proteins deposited on daily wear silicone hydrogel lenses used in combination with multipurpose disinfecting solutions (MPDSs) by applying multiple-reaction-monitoring mass spectrometry (MRM-MS). METHODS: Balafilcon A or senofilcon A contact lenses used with different MPDSs on a daily wear schedule were collected. Each worn lens was extracted and then digested with trypsin. MRM-MS was applied to quantify the amounts of lysozyme, lactoferrin, lipocalin-1, proline-rich protein-4, and keratin-1 in the extracts. RESULTS: The amount of protein extracted from the contact lenses was affected by the individual wearers, lens material, and type of care system used. Higher amounts of proteins were extracted from lenses after wear when they were used with an MPDS containing polyhexamethylene biguanide (PHMB) and poloxamer 407 compared with MPDSs containing polyquaternium-1 (PQ-1)/alexidine dihydrochloride with Tetronic 904 or PQ-1/ PHMB with poloxamine and sulfobetaine (p<0.05). There was a correlation between the amount of lipocalin-1 or keratin-1 extracted from lenses and symptoms of ocular dryness. CONCLUSIONS: The MRM-MS technique is a promising approach that could be used to reveal associations of individual proteins deposited on lenses with performance of contact lenses during wear.
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spelling pubmed-35809892013-02-25 Quantification of individual proteins in silicone hydrogel contact lens deposits Omali, Negar Babaei Zhao, Zhenjun Zhu, Hua Tilia, Daniel Willcox, Mark D.P. Mol Vis Research Article PURPOSE: The aim of this study was to quantify specific proteins deposited on daily wear silicone hydrogel lenses used in combination with multipurpose disinfecting solutions (MPDSs) by applying multiple-reaction-monitoring mass spectrometry (MRM-MS). METHODS: Balafilcon A or senofilcon A contact lenses used with different MPDSs on a daily wear schedule were collected. Each worn lens was extracted and then digested with trypsin. MRM-MS was applied to quantify the amounts of lysozyme, lactoferrin, lipocalin-1, proline-rich protein-4, and keratin-1 in the extracts. RESULTS: The amount of protein extracted from the contact lenses was affected by the individual wearers, lens material, and type of care system used. Higher amounts of proteins were extracted from lenses after wear when they were used with an MPDS containing polyhexamethylene biguanide (PHMB) and poloxamer 407 compared with MPDSs containing polyquaternium-1 (PQ-1)/alexidine dihydrochloride with Tetronic 904 or PQ-1/ PHMB with poloxamine and sulfobetaine (p<0.05). There was a correlation between the amount of lipocalin-1 or keratin-1 extracted from lenses and symptoms of ocular dryness. CONCLUSIONS: The MRM-MS technique is a promising approach that could be used to reveal associations of individual proteins deposited on lenses with performance of contact lenses during wear. Molecular Vision 2013-02-18 /pmc/articles/PMC3580989/ /pubmed/23441110 Text en Copyright © 2013 Molecular Vision. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Omali, Negar Babaei
Zhao, Zhenjun
Zhu, Hua
Tilia, Daniel
Willcox, Mark D.P.
Quantification of individual proteins in silicone hydrogel contact lens deposits
title Quantification of individual proteins in silicone hydrogel contact lens deposits
title_full Quantification of individual proteins in silicone hydrogel contact lens deposits
title_fullStr Quantification of individual proteins in silicone hydrogel contact lens deposits
title_full_unstemmed Quantification of individual proteins in silicone hydrogel contact lens deposits
title_short Quantification of individual proteins in silicone hydrogel contact lens deposits
title_sort quantification of individual proteins in silicone hydrogel contact lens deposits
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3580989/
https://www.ncbi.nlm.nih.gov/pubmed/23441110
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