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Quantification of individual proteins in silicone hydrogel contact lens deposits
PURPOSE: The aim of this study was to quantify specific proteins deposited on daily wear silicone hydrogel lenses used in combination with multipurpose disinfecting solutions (MPDSs) by applying multiple-reaction-monitoring mass spectrometry (MRM-MS). METHODS: Balafilcon A or senofilcon A contact le...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Vision
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3580989/ https://www.ncbi.nlm.nih.gov/pubmed/23441110 |
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author | Omali, Negar Babaei Zhao, Zhenjun Zhu, Hua Tilia, Daniel Willcox, Mark D.P. |
author_facet | Omali, Negar Babaei Zhao, Zhenjun Zhu, Hua Tilia, Daniel Willcox, Mark D.P. |
author_sort | Omali, Negar Babaei |
collection | PubMed |
description | PURPOSE: The aim of this study was to quantify specific proteins deposited on daily wear silicone hydrogel lenses used in combination with multipurpose disinfecting solutions (MPDSs) by applying multiple-reaction-monitoring mass spectrometry (MRM-MS). METHODS: Balafilcon A or senofilcon A contact lenses used with different MPDSs on a daily wear schedule were collected. Each worn lens was extracted and then digested with trypsin. MRM-MS was applied to quantify the amounts of lysozyme, lactoferrin, lipocalin-1, proline-rich protein-4, and keratin-1 in the extracts. RESULTS: The amount of protein extracted from the contact lenses was affected by the individual wearers, lens material, and type of care system used. Higher amounts of proteins were extracted from lenses after wear when they were used with an MPDS containing polyhexamethylene biguanide (PHMB) and poloxamer 407 compared with MPDSs containing polyquaternium-1 (PQ-1)/alexidine dihydrochloride with Tetronic 904 or PQ-1/ PHMB with poloxamine and sulfobetaine (p<0.05). There was a correlation between the amount of lipocalin-1 or keratin-1 extracted from lenses and symptoms of ocular dryness. CONCLUSIONS: The MRM-MS technique is a promising approach that could be used to reveal associations of individual proteins deposited on lenses with performance of contact lenses during wear. |
format | Online Article Text |
id | pubmed-3580989 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Molecular Vision |
record_format | MEDLINE/PubMed |
spelling | pubmed-35809892013-02-25 Quantification of individual proteins in silicone hydrogel contact lens deposits Omali, Negar Babaei Zhao, Zhenjun Zhu, Hua Tilia, Daniel Willcox, Mark D.P. Mol Vis Research Article PURPOSE: The aim of this study was to quantify specific proteins deposited on daily wear silicone hydrogel lenses used in combination with multipurpose disinfecting solutions (MPDSs) by applying multiple-reaction-monitoring mass spectrometry (MRM-MS). METHODS: Balafilcon A or senofilcon A contact lenses used with different MPDSs on a daily wear schedule were collected. Each worn lens was extracted and then digested with trypsin. MRM-MS was applied to quantify the amounts of lysozyme, lactoferrin, lipocalin-1, proline-rich protein-4, and keratin-1 in the extracts. RESULTS: The amount of protein extracted from the contact lenses was affected by the individual wearers, lens material, and type of care system used. Higher amounts of proteins were extracted from lenses after wear when they were used with an MPDS containing polyhexamethylene biguanide (PHMB) and poloxamer 407 compared with MPDSs containing polyquaternium-1 (PQ-1)/alexidine dihydrochloride with Tetronic 904 or PQ-1/ PHMB with poloxamine and sulfobetaine (p<0.05). There was a correlation between the amount of lipocalin-1 or keratin-1 extracted from lenses and symptoms of ocular dryness. CONCLUSIONS: The MRM-MS technique is a promising approach that could be used to reveal associations of individual proteins deposited on lenses with performance of contact lenses during wear. Molecular Vision 2013-02-18 /pmc/articles/PMC3580989/ /pubmed/23441110 Text en Copyright © 2013 Molecular Vision. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Omali, Negar Babaei Zhao, Zhenjun Zhu, Hua Tilia, Daniel Willcox, Mark D.P. Quantification of individual proteins in silicone hydrogel contact lens deposits |
title | Quantification of individual proteins in silicone hydrogel contact lens deposits |
title_full | Quantification of individual proteins in silicone hydrogel contact lens deposits |
title_fullStr | Quantification of individual proteins in silicone hydrogel contact lens deposits |
title_full_unstemmed | Quantification of individual proteins in silicone hydrogel contact lens deposits |
title_short | Quantification of individual proteins in silicone hydrogel contact lens deposits |
title_sort | quantification of individual proteins in silicone hydrogel contact lens deposits |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3580989/ https://www.ncbi.nlm.nih.gov/pubmed/23441110 |
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