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Developing a Clinical-Grade Cryopreservation Protocol for Human Testicular Tissue and Cells

Recent work in preservation of female fertility as well as new information on the nature of spermatogonial stem cells has prompted an investigation into the possibility of an effective clinical-grade procedure for the cryopreservation of testicular cells and/or tissue. Clinical-grade reagents, valid...

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Autores principales: Pacchiarotti, Jason, Ramos, Thomas, Howerton, Kyle, Greilach, Scott, Zaragoza, Karina, Olmstead, Marnie, Izadyar, Fariborz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581100/
https://www.ncbi.nlm.nih.gov/pubmed/23509810
http://dx.doi.org/10.1155/2013/930962
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author Pacchiarotti, Jason
Ramos, Thomas
Howerton, Kyle
Greilach, Scott
Zaragoza, Karina
Olmstead, Marnie
Izadyar, Fariborz
author_facet Pacchiarotti, Jason
Ramos, Thomas
Howerton, Kyle
Greilach, Scott
Zaragoza, Karina
Olmstead, Marnie
Izadyar, Fariborz
author_sort Pacchiarotti, Jason
collection PubMed
description Recent work in preservation of female fertility as well as new information on the nature of spermatogonial stem cells has prompted an investigation into the possibility of an effective clinical-grade procedure for the cryopreservation of testicular cells and/or tissue. Clinical-grade reagents, validated equipment, and protocols consistent with cGTP/cGMP standards were used in developing a procedure suitable for the safe and effective cryopreservation of human testicular cells and tissues. These procedures were designed to be compliant with the relevant FDA regulations. The procedure proved to effectively cryopreserve both testicular cells and tissue. The cryopreservation of testicular tissue was comparable in most aspects we measured to the cryopreservation of isolated cells, except that the viability of the cells from cryopreserved testicular tissue was found to be significantly higher. On the other hand, cryopreservation of cells is preferred for cell analysis, quality control, and sterility testing. This study demonstrates that testicular tissue and cells from sexual reassignment patients can be successfully cryopreserved with a clinical-grade procedure and important cell populations are not only preserved but also enriched by the process. Further studies will determine whether these findings from hormone-treated patients can be generalized to other patients.
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spelling pubmed-35811002013-03-18 Developing a Clinical-Grade Cryopreservation Protocol for Human Testicular Tissue and Cells Pacchiarotti, Jason Ramos, Thomas Howerton, Kyle Greilach, Scott Zaragoza, Karina Olmstead, Marnie Izadyar, Fariborz Biomed Res Int Research Article Recent work in preservation of female fertility as well as new information on the nature of spermatogonial stem cells has prompted an investigation into the possibility of an effective clinical-grade procedure for the cryopreservation of testicular cells and/or tissue. Clinical-grade reagents, validated equipment, and protocols consistent with cGTP/cGMP standards were used in developing a procedure suitable for the safe and effective cryopreservation of human testicular cells and tissues. These procedures were designed to be compliant with the relevant FDA regulations. The procedure proved to effectively cryopreserve both testicular cells and tissue. The cryopreservation of testicular tissue was comparable in most aspects we measured to the cryopreservation of isolated cells, except that the viability of the cells from cryopreserved testicular tissue was found to be significantly higher. On the other hand, cryopreservation of cells is preferred for cell analysis, quality control, and sterility testing. This study demonstrates that testicular tissue and cells from sexual reassignment patients can be successfully cryopreserved with a clinical-grade procedure and important cell populations are not only preserved but also enriched by the process. Further studies will determine whether these findings from hormone-treated patients can be generalized to other patients. Hindawi Publishing Corporation 2013 2013-01-14 /pmc/articles/PMC3581100/ /pubmed/23509810 http://dx.doi.org/10.1155/2013/930962 Text en Copyright © 2013 Jason Pacchiarotti et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Pacchiarotti, Jason
Ramos, Thomas
Howerton, Kyle
Greilach, Scott
Zaragoza, Karina
Olmstead, Marnie
Izadyar, Fariborz
Developing a Clinical-Grade Cryopreservation Protocol for Human Testicular Tissue and Cells
title Developing a Clinical-Grade Cryopreservation Protocol for Human Testicular Tissue and Cells
title_full Developing a Clinical-Grade Cryopreservation Protocol for Human Testicular Tissue and Cells
title_fullStr Developing a Clinical-Grade Cryopreservation Protocol for Human Testicular Tissue and Cells
title_full_unstemmed Developing a Clinical-Grade Cryopreservation Protocol for Human Testicular Tissue and Cells
title_short Developing a Clinical-Grade Cryopreservation Protocol for Human Testicular Tissue and Cells
title_sort developing a clinical-grade cryopreservation protocol for human testicular tissue and cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581100/
https://www.ncbi.nlm.nih.gov/pubmed/23509810
http://dx.doi.org/10.1155/2013/930962
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