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Dehydroepiandrosterone Protects Endothelial Cells against Inflammatory Events Induced by Urban Particulate Matter and Titanium Dioxide Nanoparticles

Particulate matter (PM) and nanoparticles (NPs) induce activation and dysfunction of endothelial cells characterized by inhibition of proliferation, increase of adhesion and adhesion molecules expression, increase of ROS production, and death. DHEA has shown anti-inflammatory and antioxidant propert...

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Autores principales: Huerta-García, Elizabeth, Montiél-Dávalos, Angélica, Alfaro-Moreno, Ernesto, Gutiérrez-Iglesias, Gisela, López-Marure, Rebeca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581121/
https://www.ncbi.nlm.nih.gov/pubmed/23484113
http://dx.doi.org/10.1155/2013/382058
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author Huerta-García, Elizabeth
Montiél-Dávalos, Angélica
Alfaro-Moreno, Ernesto
Gutiérrez-Iglesias, Gisela
López-Marure, Rebeca
author_facet Huerta-García, Elizabeth
Montiél-Dávalos, Angélica
Alfaro-Moreno, Ernesto
Gutiérrez-Iglesias, Gisela
López-Marure, Rebeca
author_sort Huerta-García, Elizabeth
collection PubMed
description Particulate matter (PM) and nanoparticles (NPs) induce activation and dysfunction of endothelial cells characterized by inhibition of proliferation, increase of adhesion and adhesion molecules expression, increase of ROS production, and death. DHEA has shown anti-inflammatory and antioxidant properties in HUVEC activated with proinflammatory agents. We evaluated if DHEA could protect against some inflammatory events produced by PM(10) and TiO(2) NPs in HUVEC. Adhesion was evaluated by a coculture with U937 cells, proliferation by crystal violet staining, and oxidative stress through DCFDA and Griess reagent. PM(10) and TiO(2) NPs induced adhesion and oxidative stress and inhibited proliferation of HUVEC; however, when particles were added in combination with DHEA, the effects previously observed were abolished independently from the tested concentrations and the time of addition of DHEA to the cultures. These results indicate that DHEA exerts significant anti-inflammatory and antioxidative effects on the damage induced by particles in HUVEC, suggesting that DHEA could be useful to counteract the harmful effects and inflammatory diseases induced by PM and NPs.
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spelling pubmed-35811212013-03-12 Dehydroepiandrosterone Protects Endothelial Cells against Inflammatory Events Induced by Urban Particulate Matter and Titanium Dioxide Nanoparticles Huerta-García, Elizabeth Montiél-Dávalos, Angélica Alfaro-Moreno, Ernesto Gutiérrez-Iglesias, Gisela López-Marure, Rebeca Biomed Res Int Research Article Particulate matter (PM) and nanoparticles (NPs) induce activation and dysfunction of endothelial cells characterized by inhibition of proliferation, increase of adhesion and adhesion molecules expression, increase of ROS production, and death. DHEA has shown anti-inflammatory and antioxidant properties in HUVEC activated with proinflammatory agents. We evaluated if DHEA could protect against some inflammatory events produced by PM(10) and TiO(2) NPs in HUVEC. Adhesion was evaluated by a coculture with U937 cells, proliferation by crystal violet staining, and oxidative stress through DCFDA and Griess reagent. PM(10) and TiO(2) NPs induced adhesion and oxidative stress and inhibited proliferation of HUVEC; however, when particles were added in combination with DHEA, the effects previously observed were abolished independently from the tested concentrations and the time of addition of DHEA to the cultures. These results indicate that DHEA exerts significant anti-inflammatory and antioxidative effects on the damage induced by particles in HUVEC, suggesting that DHEA could be useful to counteract the harmful effects and inflammatory diseases induced by PM and NPs. Hindawi Publishing Corporation 2013 2013-01-14 /pmc/articles/PMC3581121/ /pubmed/23484113 http://dx.doi.org/10.1155/2013/382058 Text en Copyright © 2013 Elizabeth Huerta-García et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Huerta-García, Elizabeth
Montiél-Dávalos, Angélica
Alfaro-Moreno, Ernesto
Gutiérrez-Iglesias, Gisela
López-Marure, Rebeca
Dehydroepiandrosterone Protects Endothelial Cells against Inflammatory Events Induced by Urban Particulate Matter and Titanium Dioxide Nanoparticles
title Dehydroepiandrosterone Protects Endothelial Cells against Inflammatory Events Induced by Urban Particulate Matter and Titanium Dioxide Nanoparticles
title_full Dehydroepiandrosterone Protects Endothelial Cells against Inflammatory Events Induced by Urban Particulate Matter and Titanium Dioxide Nanoparticles
title_fullStr Dehydroepiandrosterone Protects Endothelial Cells against Inflammatory Events Induced by Urban Particulate Matter and Titanium Dioxide Nanoparticles
title_full_unstemmed Dehydroepiandrosterone Protects Endothelial Cells against Inflammatory Events Induced by Urban Particulate Matter and Titanium Dioxide Nanoparticles
title_short Dehydroepiandrosterone Protects Endothelial Cells against Inflammatory Events Induced by Urban Particulate Matter and Titanium Dioxide Nanoparticles
title_sort dehydroepiandrosterone protects endothelial cells against inflammatory events induced by urban particulate matter and titanium dioxide nanoparticles
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581121/
https://www.ncbi.nlm.nih.gov/pubmed/23484113
http://dx.doi.org/10.1155/2013/382058
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