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Propagation of Adult SSCs: From Mouse to Human
Adult spermatogonial stem cells (SSCs) represent a distinctive source of stem cells in mammals for several reasons. First, by giving rise to spermatogenesis, SSCs are responsible for the propagation of a father's genetic material. As such, autologous SSCs have been considered for treatment of i...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581147/ https://www.ncbi.nlm.nih.gov/pubmed/23484114 http://dx.doi.org/10.1155/2013/384734 |
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author | Martin, Laura A. Seandel, Marco |
author_facet | Martin, Laura A. Seandel, Marco |
author_sort | Martin, Laura A. |
collection | PubMed |
description | Adult spermatogonial stem cells (SSCs) represent a distinctive source of stem cells in mammals for several reasons. First, by giving rise to spermatogenesis, SSCs are responsible for the propagation of a father's genetic material. As such, autologous SSCs have been considered for treatment of infertility and other purposes, including correction of inherited disorders. Second, adult spermatogonia can spontaneously produce embryonic-like stem cells in vitro, which could be used as an alternative for therapeutic, diagnostic, or drug discovery strategies for humans. Therefore, an increasing urgency is driving efforts to understand the biology of SSCs and improve techniques to manipulate them in vitro as a prerequisite to achieve the aforementioned goals. The characterization of adult SSCs also requires reproducible methods to isolate and maintain them in long-term culture. Herein, we describe recent major advances and challenges in propagation of adult SSCs from mice and humans during the past few years, including the use of unique cell surface markers and defined cultured conditions. |
format | Online Article Text |
id | pubmed-3581147 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-35811472013-03-12 Propagation of Adult SSCs: From Mouse to Human Martin, Laura A. Seandel, Marco Biomed Res Int Review Article Adult spermatogonial stem cells (SSCs) represent a distinctive source of stem cells in mammals for several reasons. First, by giving rise to spermatogenesis, SSCs are responsible for the propagation of a father's genetic material. As such, autologous SSCs have been considered for treatment of infertility and other purposes, including correction of inherited disorders. Second, adult spermatogonia can spontaneously produce embryonic-like stem cells in vitro, which could be used as an alternative for therapeutic, diagnostic, or drug discovery strategies for humans. Therefore, an increasing urgency is driving efforts to understand the biology of SSCs and improve techniques to manipulate them in vitro as a prerequisite to achieve the aforementioned goals. The characterization of adult SSCs also requires reproducible methods to isolate and maintain them in long-term culture. Herein, we describe recent major advances and challenges in propagation of adult SSCs from mice and humans during the past few years, including the use of unique cell surface markers and defined cultured conditions. Hindawi Publishing Corporation 2013 2013-01-01 /pmc/articles/PMC3581147/ /pubmed/23484114 http://dx.doi.org/10.1155/2013/384734 Text en Copyright © 2013 L. A. Martin and M. Seandel. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Article Martin, Laura A. Seandel, Marco Propagation of Adult SSCs: From Mouse to Human |
title | Propagation of Adult SSCs: From Mouse to Human |
title_full | Propagation of Adult SSCs: From Mouse to Human |
title_fullStr | Propagation of Adult SSCs: From Mouse to Human |
title_full_unstemmed | Propagation of Adult SSCs: From Mouse to Human |
title_short | Propagation of Adult SSCs: From Mouse to Human |
title_sort | propagation of adult sscs: from mouse to human |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581147/ https://www.ncbi.nlm.nih.gov/pubmed/23484114 http://dx.doi.org/10.1155/2013/384734 |
work_keys_str_mv | AT martinlauraa propagationofadultsscsfrommousetohuman AT seandelmarco propagationofadultsscsfrommousetohuman |