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Prescription Surveillance and Polymerase Chain Reaction Testing to Identify Pathogens during Outbreaks of Infection
Syndromic surveillance, including prescription surveillance, offers a rapid method for the early detection of agents of bioterrorism and emerging infectious diseases. However, it has the disadvantage of not considering definitive diagnoses. Here, we attempted to definitively diagnose pathogens using...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581269/ https://www.ncbi.nlm.nih.gov/pubmed/23509772 http://dx.doi.org/10.1155/2013/746053 |
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author | Sugiura, Hiroaki Fujimoto, Tsuguto Sugawara, Tamie Hanaoka, Nozomu Konagaya, Masami Kikuchi, Kiyoshi Hanada, Eisuke Okabe, Nobuhiko Ohkusa, Yasushi |
author_facet | Sugiura, Hiroaki Fujimoto, Tsuguto Sugawara, Tamie Hanaoka, Nozomu Konagaya, Masami Kikuchi, Kiyoshi Hanada, Eisuke Okabe, Nobuhiko Ohkusa, Yasushi |
author_sort | Sugiura, Hiroaki |
collection | PubMed |
description | Syndromic surveillance, including prescription surveillance, offers a rapid method for the early detection of agents of bioterrorism and emerging infectious diseases. However, it has the disadvantage of not considering definitive diagnoses. Here, we attempted to definitively diagnose pathogens using polymerase chain reaction (PCR) immediately after the prescription surveillance system detected an outbreak. Specimens were collected from 50 patients with respiratory infections. PCR was used to identify the pathogens, which included 14 types of common respiratory viruses and Mycoplasma pneumoniae. Infectious agents including M. pneumoniae, respiratory syncytial virus (RSV), rhinovirus, enterovirus, and parainfluenza virus were detected in 54% of patients. For the rapid RSV diagnosis kit, sensitivity was 80% and specificity was 85%. For the rapid adenovirus diagnosis kit, no positive results were obtained; therefore, sensitivity could not be calculated and specificity was 100%. Many patients were found to be treated for upper respiratory tract infections without the diagnosis of a specific pathogen. In Japan, an outbreak of M. pneumoniae infection began in 2011, and our results suggested that this outbreak may have included false-positive cases. By combining syndromic surveillance and PCR, we were able to rapidly and accurately identify causative pathogens during a recent respiratory infection outbreak. |
format | Online Article Text |
id | pubmed-3581269 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-35812692013-03-18 Prescription Surveillance and Polymerase Chain Reaction Testing to Identify Pathogens during Outbreaks of Infection Sugiura, Hiroaki Fujimoto, Tsuguto Sugawara, Tamie Hanaoka, Nozomu Konagaya, Masami Kikuchi, Kiyoshi Hanada, Eisuke Okabe, Nobuhiko Ohkusa, Yasushi Biomed Res Int Research Article Syndromic surveillance, including prescription surveillance, offers a rapid method for the early detection of agents of bioterrorism and emerging infectious diseases. However, it has the disadvantage of not considering definitive diagnoses. Here, we attempted to definitively diagnose pathogens using polymerase chain reaction (PCR) immediately after the prescription surveillance system detected an outbreak. Specimens were collected from 50 patients with respiratory infections. PCR was used to identify the pathogens, which included 14 types of common respiratory viruses and Mycoplasma pneumoniae. Infectious agents including M. pneumoniae, respiratory syncytial virus (RSV), rhinovirus, enterovirus, and parainfluenza virus were detected in 54% of patients. For the rapid RSV diagnosis kit, sensitivity was 80% and specificity was 85%. For the rapid adenovirus diagnosis kit, no positive results were obtained; therefore, sensitivity could not be calculated and specificity was 100%. Many patients were found to be treated for upper respiratory tract infections without the diagnosis of a specific pathogen. In Japan, an outbreak of M. pneumoniae infection began in 2011, and our results suggested that this outbreak may have included false-positive cases. By combining syndromic surveillance and PCR, we were able to rapidly and accurately identify causative pathogens during a recent respiratory infection outbreak. Hindawi Publishing Corporation 2013 2013-02-07 /pmc/articles/PMC3581269/ /pubmed/23509772 http://dx.doi.org/10.1155/2013/746053 Text en Copyright © 2013 Hiroaki Sugiura et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Sugiura, Hiroaki Fujimoto, Tsuguto Sugawara, Tamie Hanaoka, Nozomu Konagaya, Masami Kikuchi, Kiyoshi Hanada, Eisuke Okabe, Nobuhiko Ohkusa, Yasushi Prescription Surveillance and Polymerase Chain Reaction Testing to Identify Pathogens during Outbreaks of Infection |
title | Prescription Surveillance and Polymerase Chain Reaction Testing to Identify Pathogens during Outbreaks of Infection |
title_full | Prescription Surveillance and Polymerase Chain Reaction Testing to Identify Pathogens during Outbreaks of Infection |
title_fullStr | Prescription Surveillance and Polymerase Chain Reaction Testing to Identify Pathogens during Outbreaks of Infection |
title_full_unstemmed | Prescription Surveillance and Polymerase Chain Reaction Testing to Identify Pathogens during Outbreaks of Infection |
title_short | Prescription Surveillance and Polymerase Chain Reaction Testing to Identify Pathogens during Outbreaks of Infection |
title_sort | prescription surveillance and polymerase chain reaction testing to identify pathogens during outbreaks of infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581269/ https://www.ncbi.nlm.nih.gov/pubmed/23509772 http://dx.doi.org/10.1155/2013/746053 |
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