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Evaluation of Antioxidant and Wound Healing Potentials of Sphaeranthus amaranthoides Burm.f.

Background. Sphaeranthus amaranthoides commonly known as sivakaranthai is used in folklore medicine for the treatment of skin diseases. Methods. The antioxidant activity of the extract and its fraction was evaluated by using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, tot...

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Autores principales: Geethalakshmi, R., Sakravarthi, C., Kritika, T., Arul Kirubakaran, M., Sarada, D. V. L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581292/
https://www.ncbi.nlm.nih.gov/pubmed/23509751
http://dx.doi.org/10.1155/2013/607109
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author Geethalakshmi, R.
Sakravarthi, C.
Kritika, T.
Arul Kirubakaran, M.
Sarada, D. V. L.
author_facet Geethalakshmi, R.
Sakravarthi, C.
Kritika, T.
Arul Kirubakaran, M.
Sarada, D. V. L.
author_sort Geethalakshmi, R.
collection PubMed
description Background. Sphaeranthus amaranthoides commonly known as sivakaranthai is used in folklore medicine for the treatment of skin diseases. Methods. The antioxidant activity of the extract and its fraction was evaluated by using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, total antioxidant capacity, and total phenolic content. The tested plant extracts showed variable degrees of antioxidant activity. In the present study, methanolic extract of the whole plant of S. amaranthoides and a flavonoid fraction obtained from column chromatography were studied for wound healing activity by incorporating the sample in simple ointment base. Wound healing activity was studied in excision wound model in rats, following which, wound contraction, period of epithelization, hydroxyproline content, and collagen levels in the scab were studied. Results. Methanolic extract showed the highest antioxidant effect (72.05%) and diethyl ether extract has the least (29.34%) compared to the standard (74.53%). Treatment of wound with ointment containing 5% (w/w) methanolic extract and 5% (w/w) flavonoid fraction exhibited better wound healing activity than positive control (silver sulfadiazine). Finally, histopathology studies conformed wound healing activity in Sphaeranthus amaranthoides. The methanolic extract and flavonoid fraction exhibited good wound healing activity probably due to the presence of phenolic and flavonoid constituents. The methanolic extract and flavonoid fraction significantly enhanced the rate of wound contraction and the period of epithelialization comparable to silver sulfadiazine.
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spelling pubmed-35812922013-03-18 Evaluation of Antioxidant and Wound Healing Potentials of Sphaeranthus amaranthoides Burm.f. Geethalakshmi, R. Sakravarthi, C. Kritika, T. Arul Kirubakaran, M. Sarada, D. V. L. Biomed Res Int Research Article Background. Sphaeranthus amaranthoides commonly known as sivakaranthai is used in folklore medicine for the treatment of skin diseases. Methods. The antioxidant activity of the extract and its fraction was evaluated by using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, total antioxidant capacity, and total phenolic content. The tested plant extracts showed variable degrees of antioxidant activity. In the present study, methanolic extract of the whole plant of S. amaranthoides and a flavonoid fraction obtained from column chromatography were studied for wound healing activity by incorporating the sample in simple ointment base. Wound healing activity was studied in excision wound model in rats, following which, wound contraction, period of epithelization, hydroxyproline content, and collagen levels in the scab were studied. Results. Methanolic extract showed the highest antioxidant effect (72.05%) and diethyl ether extract has the least (29.34%) compared to the standard (74.53%). Treatment of wound with ointment containing 5% (w/w) methanolic extract and 5% (w/w) flavonoid fraction exhibited better wound healing activity than positive control (silver sulfadiazine). Finally, histopathology studies conformed wound healing activity in Sphaeranthus amaranthoides. The methanolic extract and flavonoid fraction exhibited good wound healing activity probably due to the presence of phenolic and flavonoid constituents. The methanolic extract and flavonoid fraction significantly enhanced the rate of wound contraction and the period of epithelialization comparable to silver sulfadiazine. Hindawi Publishing Corporation 2013 2013-02-03 /pmc/articles/PMC3581292/ /pubmed/23509751 http://dx.doi.org/10.1155/2013/607109 Text en Copyright © 2013 R. Geethalakshmi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Geethalakshmi, R.
Sakravarthi, C.
Kritika, T.
Arul Kirubakaran, M.
Sarada, D. V. L.
Evaluation of Antioxidant and Wound Healing Potentials of Sphaeranthus amaranthoides Burm.f.
title Evaluation of Antioxidant and Wound Healing Potentials of Sphaeranthus amaranthoides Burm.f.
title_full Evaluation of Antioxidant and Wound Healing Potentials of Sphaeranthus amaranthoides Burm.f.
title_fullStr Evaluation of Antioxidant and Wound Healing Potentials of Sphaeranthus amaranthoides Burm.f.
title_full_unstemmed Evaluation of Antioxidant and Wound Healing Potentials of Sphaeranthus amaranthoides Burm.f.
title_short Evaluation of Antioxidant and Wound Healing Potentials of Sphaeranthus amaranthoides Burm.f.
title_sort evaluation of antioxidant and wound healing potentials of sphaeranthus amaranthoides burm.f.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581292/
https://www.ncbi.nlm.nih.gov/pubmed/23509751
http://dx.doi.org/10.1155/2013/607109
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