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Soluble Flt-1 Gene Delivery in Acute Myeloid Leukemic Cells Mediating a Nonviral Gene Carrier

Vascular endothelial growth factor (VEGF) is a potent angiogenic factor involved in angiogenesis-mediated progression of acute myeloid leukemia (AML). Studies have reported the role of soluble form of fms-like tyrosine kinase (sFlT-1) delivery as an antitumor agent by inhibiting VEGF. This study inv...

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Autores principales: Amini, Razieh, Azizi Jalilian, Farid, Veerakumarasivam, Abhi, Abdullah, Syahril, Abdulamir, Ahmed S., Nadali, Fatemeh, Rosli, Rozita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581295/
https://www.ncbi.nlm.nih.gov/pubmed/23509773
http://dx.doi.org/10.1155/2013/752603
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author Amini, Razieh
Azizi Jalilian, Farid
Veerakumarasivam, Abhi
Abdullah, Syahril
Abdulamir, Ahmed S.
Nadali, Fatemeh
Rosli, Rozita
author_facet Amini, Razieh
Azizi Jalilian, Farid
Veerakumarasivam, Abhi
Abdullah, Syahril
Abdulamir, Ahmed S.
Nadali, Fatemeh
Rosli, Rozita
author_sort Amini, Razieh
collection PubMed
description Vascular endothelial growth factor (VEGF) is a potent angiogenic factor involved in angiogenesis-mediated progression of acute myeloid leukemia (AML). Studies have reported the role of soluble form of fms-like tyrosine kinase (sFlT-1) delivery as an antitumor agent by inhibiting VEGF. This study investigates the outcome of delivery of a VEGF165 antagonist, soluble vascular endothelial growth factor receptor, namely sFLT-1, mediating lipofectamine 2000 in acute myeloid leukemic cells. A recombinant plasmid expressing sFLT-1 was constructed and transfected into the K562 and HL60 cells using lipofectamine 2000 transfection reagent. sFLT-1 expression/secretion in pVAX-sFLT-1 transfected cells was verified by RT-PCR and western blot. MTS assay was carried out to evaluate the effect of sFLT-1 on human umbilical vein endothelial cells and K562 and HL60 cells in vitro. Treatment with pVAX-sFLT-1 showed no association between sFLT-1 and proliferation of infected K562 and HL60 cells, while it demonstrated a significant inhibitory impact on the proliferation of HUVECs. The results of the current study imply that the combination of nonviral gene carrier and sFLT-1 possesses the potential to provide efficient tool for the antiangiogenic gene therapy of AML.
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spelling pubmed-35812952013-03-18 Soluble Flt-1 Gene Delivery in Acute Myeloid Leukemic Cells Mediating a Nonviral Gene Carrier Amini, Razieh Azizi Jalilian, Farid Veerakumarasivam, Abhi Abdullah, Syahril Abdulamir, Ahmed S. Nadali, Fatemeh Rosli, Rozita Biomed Res Int Research Article Vascular endothelial growth factor (VEGF) is a potent angiogenic factor involved in angiogenesis-mediated progression of acute myeloid leukemia (AML). Studies have reported the role of soluble form of fms-like tyrosine kinase (sFlT-1) delivery as an antitumor agent by inhibiting VEGF. This study investigates the outcome of delivery of a VEGF165 antagonist, soluble vascular endothelial growth factor receptor, namely sFLT-1, mediating lipofectamine 2000 in acute myeloid leukemic cells. A recombinant plasmid expressing sFLT-1 was constructed and transfected into the K562 and HL60 cells using lipofectamine 2000 transfection reagent. sFLT-1 expression/secretion in pVAX-sFLT-1 transfected cells was verified by RT-PCR and western blot. MTS assay was carried out to evaluate the effect of sFLT-1 on human umbilical vein endothelial cells and K562 and HL60 cells in vitro. Treatment with pVAX-sFLT-1 showed no association between sFLT-1 and proliferation of infected K562 and HL60 cells, while it demonstrated a significant inhibitory impact on the proliferation of HUVECs. The results of the current study imply that the combination of nonviral gene carrier and sFLT-1 possesses the potential to provide efficient tool for the antiangiogenic gene therapy of AML. Hindawi Publishing Corporation 2013 2013-01-10 /pmc/articles/PMC3581295/ /pubmed/23509773 http://dx.doi.org/10.1155/2013/752603 Text en Copyright © 2013 Razieh Amini et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Amini, Razieh
Azizi Jalilian, Farid
Veerakumarasivam, Abhi
Abdullah, Syahril
Abdulamir, Ahmed S.
Nadali, Fatemeh
Rosli, Rozita
Soluble Flt-1 Gene Delivery in Acute Myeloid Leukemic Cells Mediating a Nonviral Gene Carrier
title Soluble Flt-1 Gene Delivery in Acute Myeloid Leukemic Cells Mediating a Nonviral Gene Carrier
title_full Soluble Flt-1 Gene Delivery in Acute Myeloid Leukemic Cells Mediating a Nonviral Gene Carrier
title_fullStr Soluble Flt-1 Gene Delivery in Acute Myeloid Leukemic Cells Mediating a Nonviral Gene Carrier
title_full_unstemmed Soluble Flt-1 Gene Delivery in Acute Myeloid Leukemic Cells Mediating a Nonviral Gene Carrier
title_short Soluble Flt-1 Gene Delivery in Acute Myeloid Leukemic Cells Mediating a Nonviral Gene Carrier
title_sort soluble flt-1 gene delivery in acute myeloid leukemic cells mediating a nonviral gene carrier
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581295/
https://www.ncbi.nlm.nih.gov/pubmed/23509773
http://dx.doi.org/10.1155/2013/752603
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