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Immobilization of Heparan Sulfate on Electrospun Meshes to Support Embryonic Stem Cell Culture and Differentiation

As our understanding of what guides the behavior of multi- and pluripotent stem cells deepens, so too does our ability to utilize certain cues to manipulate their behavior and maximize their therapeutic potential. Engineered, biologically functionalized materials have the capacity to influence stem...

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Autores principales: Meade, Kate A., White, Kathryn J., Pickford, Claire E., Holley, Rebecca J., Marson, Andrew, Tillotson, Donna, van Kuppevelt, Toin H., Whittle, Jason D., Day, Anthony J., Merry, Catherine L. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581394/
https://www.ncbi.nlm.nih.gov/pubmed/23235146
http://dx.doi.org/10.1074/jbc.M112.423012
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author Meade, Kate A.
White, Kathryn J.
Pickford, Claire E.
Holley, Rebecca J.
Marson, Andrew
Tillotson, Donna
van Kuppevelt, Toin H.
Whittle, Jason D.
Day, Anthony J.
Merry, Catherine L. R.
author_facet Meade, Kate A.
White, Kathryn J.
Pickford, Claire E.
Holley, Rebecca J.
Marson, Andrew
Tillotson, Donna
van Kuppevelt, Toin H.
Whittle, Jason D.
Day, Anthony J.
Merry, Catherine L. R.
author_sort Meade, Kate A.
collection PubMed
description As our understanding of what guides the behavior of multi- and pluripotent stem cells deepens, so too does our ability to utilize certain cues to manipulate their behavior and maximize their therapeutic potential. Engineered, biologically functionalized materials have the capacity to influence stem cell behavior through a powerful combination of biological, mechanical, and topographical cues. Here, we present the development of a novel electrospun scaffold, functionalized with glycosaminoglycans (GAGs) ionically immobilized onto the fiber surface. Bound GAGs retained the ability to interact with GAG-binding molecules and, crucially, presented GAG sulfation motifs fundamental to mediating stem cell behavior. Bound GAG proved to be biologically active, rescuing the neural differentiation capacity of heparan sulfate-deficient mouse embryonic stem cells and functioning in concert with FGF4 to facilitate the formation of extensive neural processes across the scaffold surface. The combination of GAGs with electrospun scaffolds creates a biomaterial with potent applicability for the propagation and effective differentiation of pluripotent stem cells.
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spelling pubmed-35813942013-02-27 Immobilization of Heparan Sulfate on Electrospun Meshes to Support Embryonic Stem Cell Culture and Differentiation Meade, Kate A. White, Kathryn J. Pickford, Claire E. Holley, Rebecca J. Marson, Andrew Tillotson, Donna van Kuppevelt, Toin H. Whittle, Jason D. Day, Anthony J. Merry, Catherine L. R. J Biol Chem Glycobiology and Extracellular Matrices As our understanding of what guides the behavior of multi- and pluripotent stem cells deepens, so too does our ability to utilize certain cues to manipulate their behavior and maximize their therapeutic potential. Engineered, biologically functionalized materials have the capacity to influence stem cell behavior through a powerful combination of biological, mechanical, and topographical cues. Here, we present the development of a novel electrospun scaffold, functionalized with glycosaminoglycans (GAGs) ionically immobilized onto the fiber surface. Bound GAGs retained the ability to interact with GAG-binding molecules and, crucially, presented GAG sulfation motifs fundamental to mediating stem cell behavior. Bound GAG proved to be biologically active, rescuing the neural differentiation capacity of heparan sulfate-deficient mouse embryonic stem cells and functioning in concert with FGF4 to facilitate the formation of extensive neural processes across the scaffold surface. The combination of GAGs with electrospun scaffolds creates a biomaterial with potent applicability for the propagation and effective differentiation of pluripotent stem cells. American Society for Biochemistry and Molecular Biology 2013-02-22 2012-12-12 /pmc/articles/PMC3581394/ /pubmed/23235146 http://dx.doi.org/10.1074/jbc.M112.423012 Text en © 2013 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version full access. Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) applies to Author Choice Articles
spellingShingle Glycobiology and Extracellular Matrices
Meade, Kate A.
White, Kathryn J.
Pickford, Claire E.
Holley, Rebecca J.
Marson, Andrew
Tillotson, Donna
van Kuppevelt, Toin H.
Whittle, Jason D.
Day, Anthony J.
Merry, Catherine L. R.
Immobilization of Heparan Sulfate on Electrospun Meshes to Support Embryonic Stem Cell Culture and Differentiation
title Immobilization of Heparan Sulfate on Electrospun Meshes to Support Embryonic Stem Cell Culture and Differentiation
title_full Immobilization of Heparan Sulfate on Electrospun Meshes to Support Embryonic Stem Cell Culture and Differentiation
title_fullStr Immobilization of Heparan Sulfate on Electrospun Meshes to Support Embryonic Stem Cell Culture and Differentiation
title_full_unstemmed Immobilization of Heparan Sulfate on Electrospun Meshes to Support Embryonic Stem Cell Culture and Differentiation
title_short Immobilization of Heparan Sulfate on Electrospun Meshes to Support Embryonic Stem Cell Culture and Differentiation
title_sort immobilization of heparan sulfate on electrospun meshes to support embryonic stem cell culture and differentiation
topic Glycobiology and Extracellular Matrices
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581394/
https://www.ncbi.nlm.nih.gov/pubmed/23235146
http://dx.doi.org/10.1074/jbc.M112.423012
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